FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205697
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55980.4642957558988701No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44940.3727304621310329No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT40790.3383105373904057No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39180.3249572653825961No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37840.3138433619723695No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33320.2763546728572768No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32940.2732029689051229No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32760.2717100565067343No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31180.2586056032319895No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30060.24931637053090452No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28260.23438724654701806No Hit
GCCTAGAGCCTGAGGATTCTGCAATTTATTACTGTCAGCAGCGTGAGCACTGGTTGCTCACTTTCGGCGGAGGGA28220.23405548823626499No Hit
CTACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG26770.22202924947146754No Hit
TCCTTAGCCTGGTATCAACACAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCATCCTACAGGGCCA26760.22194630989377928No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26600.22061927665076717No Hit
GTAATAGACAGCCGTGTCTTCGGGTCGCAGGCTGCCCATTTGCAAATATA26340.21846284763087243No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25890.2147305666349008No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGATGTTCGCAC25620.2124911980373178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25080.2080124608421519No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25060.2078465816867754No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24980.2071830650652693No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24560.20369960280236243No Hit
GTCCCAGACCCACCGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTA24540.2035337236469859No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24150.20029908011714387No Hit
CTCCTGCAGGGCCAGTCAGGATGTTCGCACCTCCTTAGCCTGGTATCAACACAAACCTGGCCAGGCTCCCAGGCT23510.19499094714509532No Hit
GTCTATTACTGTGCGAAAGCCGGGGGTGAATACAATGAGTACGCCACTCTTTTTGGCTCCTGGGGCCAGGGAACC22870.1896828141730468No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGG22760.18877047881847595No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22550.18702874768702255No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22450.18619935191013995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22050.18288176880260962No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTCTGCAATTTA21440.17782245456362586No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21150.17541720681066636No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20500.17002613426092958No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCAACCAGTG18580.15410173534478397No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCATCCTACAGGGCCACTGGCATCCCAGGCAGGTT18470.15318939999021314No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18320.15194530632488926No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17720.14696893166359373No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17650.14638835461977595No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17420.14448074433294603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16820.1395043696716505No Hit
GGCTAAGGAGGTGCGAACATCCTGACTGGCCCTGCAGGAGAGGGTGGCTC16730.1387579134724562No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16680.13834321558401488No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16400.13602090740874365No Hit
GTTCAGTGGCGGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC15730.13046395570363034No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15520.12872222457217694No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGAACCTGCCTGGGATGCCA15470.12830752668373563No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15460.12822458710604737No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15390.12764401006222956No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15120.12540464146464658No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14950.12399466864394619No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14940.12391172906625794No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14530.12051120638103935No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14430.11968181060415675No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14410.11951593144878024No Hit
GTGCTCACGCTGCTGACAGTAATAAATTGCAGAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT14090.11686186496275598No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC14060.11661304622969121No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13970.11586659003049687No Hit
GCGTGAGCACTGGTTGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13950.11570071087512036No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13940.1156177712974321No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13940.1156177712974321No Hit
GTATCAACACAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCATCCTACAGGGCCACTGGCATCCCAGG13930.11553483171974385No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13810.11453955678748474No Hit
GTCAGGATGTTCGCACCTCCTTAGCCTGGTATCAACACAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATG13810.11453955678748474No Hit
GCCCTGTAGGATGCATCATAGATGACGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGATACCAGGCTAAGGAGGTG13770.11420779847673172No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGC13430.11138785283533092No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13300.11030963832538358No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13160.10914848423774796No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13080.10848496761624189No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12910.10707499479554151No Hit
ATTTATTACTGTCAGCAGCGTGAGCACTGGTTGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12660.10500150535333504No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12590.10442092830951723No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12470.10342565337725813No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12410.10292801591112859No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12160.10085452646892212No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12130.10060570773585734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)