FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472851
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGTTGCTCATTTACTGGGCATCAACCCGGGAATCCGGGGTCCCT66810.4536100393047226No Hit
ATTTTATACGGCTCCAACGAGAGGAACTACGTAAGTTGGTACCAGCAGAA64540.4381977538800598No Hit
ACCTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTACGTAGTTCCTCTCGTTGGAGCCGTATAAAATAGT64410.43731511198349327No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52420.3559083709078515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50690.3441624441304653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40780.27687797339988907No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38910.2641815091954312No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36970.2510097762774374No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35210.23906016290853588No Hit
GTATAAAATAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA33700.2288079378022624No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32810.22276523558730651No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31000.21047614456587938No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30900.20979718926082816No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30050.20402606916789276No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28640.19445279936667048No Hit
AACCAGGACAGTCTCCTAGGTTGCTCATTTACTGGGCATCAACCCGGGAA28620.19431700830566026No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28460.19323067981757827No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28300.19214435132949634No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAATGTC28200.19146539602444512No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27830.18895326139575558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27660.1877990373771685No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC25650.1741520357456389No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24960.16946724414078546No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCC24640.16729458716462153No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23350.15853606372946075No Hit
GTCTCCTAGGTTGCTCATTTACTGGGCATCAACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT22550.15310442128905094No Hit
GTATTTTTATATTCCGTGGACATTCGGCCAAGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCAT22530.15296863022804072No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTATTTTATA22260.15113545090440242No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAGTATTTTTATATT22220.15086386878238192No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21880.14855542074520778No Hit
GTCCTGGTTTCTGCTGGTACCAACTTACGTAGTTCCTCTCGTTGGAGCCG21780.14787646544015653No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTATTTTATACGGCTCCAA20790.14115480792014942No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19920.13524789676620377No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGCGAC19870.13490841911367818No Hit
GTCAGGGACCCCGGATTCCCGGGTTGATGCCCAGTAAATGAGCAACCTAGGAGACTGTCCTGGTTTCTGCTGGTA19720.13388998615610131No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19580.13293944872902963No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19110.12974835879528887No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG19020.12913729902074275No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18470.12540304484296103No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18100.12289091021427151No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18080.12275511915326126No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17700.12017508899406659No Hit
TTTATATTCCGTGGACATTCGGCCAAGGGACCAAGGTAGAAATCAAACGA17520.11895296944497441No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17420.11827401413992318No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17390.11807032754840782No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17240.11705189459083097No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17090.11603346163325415No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACAGTATTTTTATATTCCGTGGACATT16870.11453975996214147No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16840.11433607337062608No Hit
GTTTATTTCTGTCAACAGTATTTTTATATTCCGTGGACATTCGGCCAAGGGACCAAGGTAGAAATCAAACGAA16800.1140644912486056No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16690.11331764041304926No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16480.11189183427244168No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16470.11182393874193655No Hit
GAATATAAAAATACTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT16370.11114498343688534No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15970.10842916221668045No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15870.1077502069116292No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15810.10734283372859847No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15790.10720704266758824No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15650.10625650524051652No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15620.10605281864900117No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15500.10523807228293969No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15480.10510228122192944No Hit
GTAAATGAGCAACCTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTA15430.10476280356940383No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15410.10462701250839358No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15400.10455911697788846No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTATTTTATACGGCTCCAACGAGAGGAACTA15210.10326910189829115No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15070.10231856447121943No Hit
ACGTAAGTTGGTACCAGCAGAAACCAGGACAGTCTCCTAGGTTGCTCATT14950.10150381810515796No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14910.10123223598313748No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14790.100417489617076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)