FastQCFastQC Report
Wed 8 May 2024
EGAF00004691988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472851
Total Bases108.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGTTGCTCATTTACTGGGCATCAACCCGGGAATCCGGGGTCCCT64290.43650036561743183No Hit
ATTTTATACGGCTCCAACGAGAGGAACTACGTAAGTTGGTACCAGCAGAA62490.42427917012650973No Hit
ACCTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTACGTAGTTCCTC61970.4207486025402434No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT57450.3900598227519281No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51180.34748932512521635No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42830.29079655715343916No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42380.2877412582807086No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40000.27158212202048954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39720.2696810471663461No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35370.24014649139661787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35310.23973911821358712No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA32760.22242575793478092No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32600.22133942944669893No Hit
GTATAAAATAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA32030.21746938420790698No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31670.21502514510972257No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC31050.21081562221840497No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAATGTC30860.20952560713880766No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA30780.2089824428947667No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29030.19710072505637025No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC27620.18752745525514802No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27460.18644112676706603No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC27280.18521900721797385No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG26900.1826389770587792No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCC26850.1822994994062536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26680.1811452753876665No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC25490.17306570725755696No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT24800.1683809156527035No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24680.16756616928664203No Hit
AACCAGGACAGTCTCCTAGGTTGCTCATTTACTGGGCATCAACCCGGGAA24560.16675142292058054No Hit
GTATTTTTATATTCCGTGGACATTCGGCCAAGGGACCAAGGTAGAAATCA23540.15982607880905808No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGCGAC23530.15975818327855296No Hit
GTCCTGGTTTCTGCTGGTACCAACTTACGTAGTTCCTCTCGTTGGAGCCG23400.15887554138198637No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA23010.1562276156922866No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG22060.14977754029429996No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA21860.14841962968419753No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC21410.14536433081146702No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG21310.14468537550641578No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21200.14393852467085944No Hit
GTCTCCTAGGTTGCTCATTTACTGGGCATCAACCCGGGAATCCGGGGTCC21120.14339536042681847No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT20670.14034006155408796No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA20460.1389142554134804No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT20350.13816740457792404No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA20270.13762424033388307No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19920.13524789676620377No Hit
GTCAGGGACCCCGGATTCCCGGGTTGATGCCCAGTAAATGAGCAACCTAG19840.1347047325221628No Hit
GAATATAAAAATACTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGC19470.1321925978934733No Hit
TTTATATTCCGTGGACATTCGGCCAAGGGACCAAGGTAGAAATCAAACGA18430.12513146272094053No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA18390.12485988059892006No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAACAGTATTTTTATATTCCGT18240.12384144764134321No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTC18130.12309459680578687No Hit
GTTTATTTCTGTCAACAGTATTTTTATATTCCGTGGACATTCGGCCAAGG17940.12180458172618953No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17690.1201071934635615No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17680.12003929793305637No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17630.11969982028053076No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17630.11969982028053076No Hit
ATAAAAATACTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGC17310.11752716330436684No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA17180.11664452140780024No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17170.11657662587729511No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17110.11616925269426438No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17050.11576187951123365No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16890.11467555102315169No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16790.11399659571810047No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC16630.11291026723001853No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16630.11291026723001853No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC16610.11277447616900828No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16550.11236710298597753No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAAC16420.11148446108941094No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG16250.11033023707082386No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT16210.11005865494880337No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC16120.10944759517425727No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16070.10910811752173166No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16040.1089044309302163No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15960.10836126668617532No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15680.1064601918320319No Hit
GTAAATGAGCAACCTAGGAGACTGTCCTGGTTTCTGCTGGTACCAACTTA15430.10476280356940383No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT15260.10360857955081676No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14970.10163960916616821No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14890.10109644492212722No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTATTT14750.1001459074950555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)