FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219661
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC92650.759637309055549No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC65510.5371164610494228No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA60780.4983351931397331No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTACCA56800.46570317489859886No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG53400.4378265764011476No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49560.4063424180981437No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46050.3775639296493042No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42950.352147031019275No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG40240.3299277422168947No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA40180.32943580224341024No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC40140.3291078422610873No Hit
CTACTACTGTTACTCAAGAGACAGCAGTGGTAATCATTGGGTGTTCGGCG39920.32730406235831105No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT38530.3159074529725883No Hit
CCCCTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCT37970.3113160132200669No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37140.30451084358686553No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36570.2998374138387634No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT35550.29147443428952796No Hit
CAGTAGTAGTCAGCCTCATCGTCCACCTGGGCCCCACTGATAGTCAAGGA34750.28491523464306884No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34230.2806517548728704No Hit
TCACAGGGGCCTGCCCTGACTTTTGCTGGTACCAATAAGGCCATTCTTTT34160.28007782490380523No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC33780.27696220507173713No Hit
GACTACTACTGTTACTCAAGAGACAGCAGTGGTAATCATTGGGTGTTCGG33690.2762242951115105No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG31650.2594983360130397No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31540.25859644606165155No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC30890.2532670963489035No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30780.25236520639751536No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG30180.24744580666267105No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29890.24506809679082958No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29520.24203446695434225No Hit
GTAGTAGTCAGCCTCATCGTCCACCTGGGCCCCACTGATAGTCAAGGAGG29380.24088660701621187No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29010.23785297717972453No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA28240.2315397475200076No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27260.22350472795309517No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26950.22096303809009227No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTACCAC26120.2141578684568909No Hit
GCTCTGGAGATGCATTGCCAAAAGAATGGCCTTATTGGTACCAGCAAAAG25690.2106322986469191No Hit
GGGCAGGCCCCTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGG25430.2085005587618199No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24390.199973599221423No Hit
GCCTTATTGGTACCAGCAAAAGTCAGGGCAGGCCCCTGTGATGGTCATCG24160.19808782932306598No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC23350.19144663968102613No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22820.18710116991524695No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22710.1861992799638588No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22310.18291968014062923No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22070.18095192024669152No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21710.1780002804057849No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21320.17480267057813606No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21280.1744747105958131No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAGAATGGCCTTATTGGTACCAGCAAAAGTCAGGGCAGGCCC20970.17193302073281017No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20670.16947332086538802No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20320.16660367102006213No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20260.1661117310465777No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19440.15938855140895708No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18870.15471512166085494No Hit
GCTCAGGGACAATGGCCTCCTTGACTATCAGTGGGGCCCAGGTGGACGAT18570.15225542179343277No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18200.1492217919569454No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17680.14495831218674698No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17640.14463035220442402No Hit
GTCCCTGAGCTGGAGGCAGAGAATCTCTCAGGGATCCCGGAGGGTCGGTT17300.14184269235467886No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17000.13938299248725672No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA16660.13659533263751159No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16550.13569344268612343No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT16410.13454558274799308No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16320.13380767278776642No Hit
GGAGATGCATTGCCAAAAGAATGGCCTTATTGGTACCAGCAAAAGTCAGG15920.13052807296453686No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG15800.129544193017568No Hit
CCCAATGATTACCACTGCTGTCTCTTGAGTAACAGTAGTAGTCAGCCTCA15630.12815036309269542No Hit
GACTATCAGTGGGGCCCAGGTGGACGATGAGGCTGACTACTACTGTTACT15490.12700250315456507No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15430.12651056318108064No Hit
CTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCTGAGAGATTCTCTGCCTCCAGCTCAGGGA15410.12634658318991918No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC15380.12610061320317695No Hit
GGCCTGCCCTGACTTTTGCTGGTACCAATAAGGCCATTCTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC15370.12601862320759621No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15330.12569066322527325No Hit
ACTCAAGAGACAGCAGTGGTAATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG15250.12503474326062736No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC15180.12446081329156215No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15150.12421484330481995No Hit
GATTACCACTGCTGTCTCTTGAGTAACAGTAGTAGTCAGCCTCATCGTCC15150.12421484330481995No Hit
CTGATAGTCAAGGAGGCCATTGTCCCTGAGCTGGAGGCAGAGAATCTCTC15130.12405086331365846No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15120.12396887331807772No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15120.12396887331807772No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG14930.12241106340204369No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC14890.12208310341972073No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTAC14880.12200111342414No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14840.12167315344181703No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14770.12109922347275186No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC14690.12044330350810593No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14660.12019733352136373No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT14620.11986937353904077No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14560.11937743356555633No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14550.1192954435699756No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGATTACCACTGCTGTCTCTTGAGTAACAGTAGTAGTCAGCCTCATCGT14510.11896748358765263No Hit
GTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCTGAGAG14380.11790161364510303No Hit
GTGGTAATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA14340.11757365366278007No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14270.1169997236937149No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14200.11642579372464971No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14100.11560589376884231No Hit
GCCAAAAGAATGGCCTTATTGGTACCAGCAAAAGTCAGGGCAGGCCCCTGTGATGGTCATCGATGTCAACGGCAA13890.11388410386164681No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13710.1124082839411935No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG13680.11216231395445128No Hit
AGTCAGGGCAGGCCCCTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCTGAGAGATTCTCTG13570.11126042400306316No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGACGATGAGGCTGACTACTACTGT13380.1097026140870291No Hit
CCATCACAGGGGCCTGCCCTGACTTTTGCTGGTACCAATAAGGCCATTCT13320.10921067411354467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.10650500425938027No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG12940.10609505428147657No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12930.10601306428589584No Hit
AGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGC12890.10568510430357288No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12740.10445525436986178No Hit
GGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAGAATG12730.10437326437428104No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12670.10388132440079662No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12640.10363535441405439No Hit
GACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCA12530.10273346446266626No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC12330.10109366455105148No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT12300.10084769456430925No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA12250.10043774458640556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)