FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219661
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC93240.7644747187948127No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC62480.5122734923884588No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA57380.47045859464228174No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG52380.42946359685191215No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTACCA50490.41396748768715247No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48360.39650361862845496No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43970.36051001056851045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39800.32632018241134214No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG38100.3123818831626165No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38020.3117259631979706No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT37460.30713452344544917No Hit
CTACTACTGTTACTCAAGAGACAGCAGTGGTAATCATTGGGTGTTCGGCG36970.30311701366199295No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36950.3029530336708315No Hit
CCCCTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCT36690.3008212937857323No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC36340.2979516439404064No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT36050.29557393406856497No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG35580.2917204042762702No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG33780.27696220507173713No Hit
GACTACTACTGTTACTCAAGAGACAGCAGTGGTAATCATTGGGTGTTCGG32630.2675333555799521No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG32160.2636798257876574No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC32120.26335186580533443No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32010.2624499758539463No Hit
CAGTAGTAGTCAGCCTCATCGTCCACCTGGGCCCCACTGATAGTCAAGGA31430.25769455611026343No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30670.25146331644612724No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29800.24433018683060292No Hit
GTAGTAGTCAGCCTCATCGTCCACCTGGGCCCCACTGATAGTCAAGGAGG29310.24031267704714673No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29170.23916481710901635No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29060.2382629271576282No Hit
TCACAGGGGCCTGCCCTGACTTTTGCTGGTACCAATAAGGCCATTCTTTT28680.23514730732556013No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28650.23490133733881793No Hit
GGGCAGGCCCCTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGG26390.21637159833757086No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25550.20948443870878874No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA25460.2087465287485621No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC25250.20702473884136657No Hit
GCTCTGGAGATGCATTGCCAAAAGAATGGCCTTATTGGTACCAGCAAAAG24700.20251528908442593No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTACCAC23970.19653001940703194No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23810.19521817947774014No Hit
GCCTTATTGGTACCAGCAAAAGTCAGGGCAGGCCCCTGTGATGGTCATCG23610.19357837956612534No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23580.1933324095793831No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23280.19087270971196094No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22640.18562534999479363No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22590.18521540001688994No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC22560.18496943003014774No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22030.18062396026436855No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC21700.17791829041020416No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAGAATGGCCTTATTGGTACCAGCAAAAGTCAGGGCAGGCCC21410.1755405805383627No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20680.16955531086096876No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20600.16889939089632283No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20480.16791551094935395No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19310.1583226814664075No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19300.15824069147082673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18890.15487910165201643No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18630.1527473617669172No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18410.15094358186414095No Hit
GTGGTAATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA18040.14790995202765359No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17430.14290856229722848No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA17020.13954697247841819No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG17000.13938299248725672No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16840.13807115255796487No Hit
GCTCAGGGACAATGGCCTCCTTGACTATCAGTGGGGCCCAGGTGGACGAT16580.13593941267286566No Hit
GTCCCTGAGCTGGAGGCAGAGAATCTCTCAGGGATCCCGGAGGGTCGGTT16530.13552946269496197No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16520.13544747269938123No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16450.13487354273031604No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16070.13175792289824795No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16030.13142996291592499No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC16020.13134797292034425No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC15810.12962618301314874No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15750.1291342430396643No Hit
CTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCTGAGAGATTCTCTGCCTCCAGCTCAGGGA15610.12798638310153396No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15550.1274944431280495No Hit
GGCCTGCCCTGACTTTTGCTGGTACCAATAAGGCCATTCTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC15440.12659255317666138No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15430.12651056318108064No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15400.12626459319433841No Hit
GGAGATGCATTGCCAAAAGAATGGCCTTATTGGTACCAGCAAAAGTCAGG15400.12626459319433841No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC15340.12577265322085399No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14960.12265703338878589No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14910.1222470834108822No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14830.1215911634462363No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC14790.12126320346391332No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATTAC14770.12109922347275186No Hit
GATTACCACTGCTGTCTCTTGAGTAACAGTAGTAGTCAGCCTCATCGTCC14720.12068927349484816No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14690.12044330350810593No Hit
CTGATAGTCAAGGAGGCCATTGTCCCTGAGCTGGAGGCAGAGAATCTCTC14420.11822957362742598No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14410.11814758363184524No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14110.11568788376442307No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT13980.11462201382187345No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGATTACCACTGCTGTCTCTTGAGTAACAGTAGTAGTCAGCCTCATCGT13880.11380211386606606No Hit
ACTCAAGAGACAGCAGTGGTAATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13840.11347415388374311No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13810.11322818389700089No Hit
GTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCTGAGAG13700.11232629394561276No Hit
CCCAATGATTACCACTGCTGTCTCTTGAGTAACAGTAGTAGTCAGCCTCA13690.11224430395003201No Hit
GACTATCAGTGGGGCCCAGGTGGACGATGAGGCTGACTACTACTGTTACT13630.11175236397654759No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13550.11109644401190166No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13520.11085047402515946No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC13300.1090466941223832No Hit
GCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCT13300.1090466941223832No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG13290.10896470412680244No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13220.10839077415773726No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13130.1076528641975106No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12960.10625903427263804No Hit
AGTCAGGGCAGGCCCCTGTGATGGTCATCGATGTCAACGGCAACCGACCCTCCGGGATCCCTGAGAGATTCTCTG12850.10535714432124992No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGACGATGAGGCTGACTACTACTGT12830.10519316433008845No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC12800.10494719434334622No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12710.10420928438311958No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12470.10224152448918182No Hit
GGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAGAATG12350.10125764454221296No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12340.10117565454663222No Hit
GCCAAAAGAATGGCCTTATTGGTACCAGCAAAAGTCAGGGCAGGCCCCTGTGATGGTCATCGATGTCAACGGCAA12270.10060172457756705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)