FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708772
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37080.5231583640437264No Hit
ATCCTATATTGACTTTGCACAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA33920.47857421004215744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31900.4500742128639393No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24300.3428465007082672No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23880.336920758720717No Hit
ACAATATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA23280.32845541302421655No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22030.31081927615650734No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT22000.3103960088716823No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21950.30969056339697393No Hit
ATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACTTAAATAGTTGCCAATGGT21570.30432917778919033No Hit
GAGTTACAATATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT20550.2899380901051396No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20020.2824603680732309No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19490.27498264604132217No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT18770.2648242312055217No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18680.2635544293510466No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18440.26016829107244643No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18060.25480690546466284No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17980.2536781927051294No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17650.2490222525720542No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17500.2469059161479291No Hit
GGATATTGTAACTCTGTTGACAGAAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT17300.2440841342490956No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16900.2384405704514287No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC16690.2354776994576535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16230.22898760109033653No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCAA15820.22320294819772793No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCAACTATTTAAGTTGGTATCAGCAGAAG15720.22179205724831116No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC15520.2189702753494777No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15170.21403215702651912No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14790.20867077141873552No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14530.205002454950252No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14260.20119304938682678No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC13620.19216334731055967No Hit
GGACCCCACTGTGCAAAGTCAATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAA13560.19131681274090964No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13000.1834158234241759No Hit
CTATTTAAGTTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAGCCTCCTGA12880.1817227542848758No Hit
TTGTAACTCTGTTGACAGAAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC12810.1807351306202841No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12770.18017077424051742No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12480.17607919048720885No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTTCTGTCAACAGAGTTACAATATC12410.17509156682261715No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12220.17241087401872535No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11970.16888364664518352No Hit
GATCTATCCTATATTGACTTTGCACAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11830.16690839931600007No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11830.16690839931600007No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11530.16267572646774986No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11280.159148499094208No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC11260.15886632090432465No Hit
GGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACT10580.14927226244829084No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10350.14602721326463236No Hit
GTTACAATATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA10230.14433414412533227No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10190.14376978774556556No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10060.14193562951132382No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9960.14052473856190706No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9960.14052473856190706No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9940.14024256037202373No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9560.1348811747642401No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9360.13205939286540666No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9320.13149503648563995No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA9110.1285321654918648No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9050.12768563092221474No Hit
GTCAATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTG9000.1269801854475064No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8910.12571038359303133No Hit
GTGCAAAGTCAATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCT8910.12571038359303133No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8900.12556929449808965No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8840.1247227599284396No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8820.12444058173855628No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC8790.12401731445373125No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8780.12387622535878957No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8770.1237351362638479No Hit
CTTAAATAGTTGCCAATGGTCTGACTTGCCCGGCAAGTGATGGTGACTCT8670.12232424531443116No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8520.12020790889030605No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8440.11907919613077267No Hit
CCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCG8430.11893810703583099No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8390.11837375065606429No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8240.1162574142319392No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTTCTGTCAACAGAGTTACAATATCCCGTGGACGTT8220.11597523604205584No Hit
GACTTTGCACAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGA8190.11555196875723081No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8120.1145643450926391No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8050.11357672142804738No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTTCTGTCAACAGAGTTACAATATCCCGTGGACGTTCGGCCAAGGGA8030.11329454323816403No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8000.11287127595333903No Hit
GATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACTTAAAT7990.11273018685839735No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7970.112448008668514No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7940.11202474138368897No Hit
GGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACTTAAATAGTTGCCA7910.11160147409886396No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7840.11061385043427223No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCA7820.11033167224438888No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG7750.10934404857979717No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7720.10892078129497215No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7670.10821533582026378No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGGATATTGTAACTCTGTTGACAG7580.10694553396578871No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7550.1065222666809637No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7540.10638117758602203No Hit
CTCCTGATCTATCCTATATTGACTTTGCACAGTGGGGTCCCGTCAAGGTT7440.10497028663660529No Hit
AGTCAGACCATTGGCAACTATTTAAGTTGGTATCAGCAGAAGCCAGGGAA7430.10482919754166362No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7410.10454701935178026No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7390.10426484116189691No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7360.10384157387707188No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG7240.1021485047377718No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7210.10172523745294679No Hit
CTATATTGACTTTGCACAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGA7130.10059652469341339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT150.002594828167.333994
GACGCCT200.00807042250.55399325
GTATAAC200.00809297850.518321
GAGCCTA3800.049.03645331
CCGTCTT3800.048.8737647
CGTATGC3750.047.8530441
TCGTATG3750.047.7391140
CTTATAC3900.047.495851
CACGAGA3900.047.48914721
TATGCCG3900.047.13643
TATACAC4000.047.1337973
ACGAGAC3950.046.8946422
GCCTATC3700.046.81542633
GACCGGA4050.046.6440426
CGTCTTC4000.046.55313548
TATCTCG3750.046.40590736
ATGCCGT3900.046.3544644
AGACCGG4000.046.3411625
CCCACGA4000.046.30518719
CTTGAAA4050.046.27264857
Produced by FastQC (version 0.11.5)