FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708772
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41260.582133605729346No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37510.5292251951262184No Hit
ATCCTATATTGACTTTGCACAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA33840.4774454972826241No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25580.36090590486080154No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT25530.36020045938609313No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24130.3404479860942588No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24080.33974254061955045No Hit
ATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACTTAAATAGTTGCCAATGGT21730.3065866033082571No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21710.30630442511837375No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21400.30193066317518186No Hit
ACAATATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA21050.29699254485222326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20220.28528214997206436No Hit
GAGTTACAATATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT18110.2555123509393712No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17960.25339601451524607No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCAA16930.2388638377362537No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16430.23180938298916998No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16140.22771779923586147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16130.2275767101409198No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16070.22673017557126976No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC15920.22461383914714464No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15830.22334403729266958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15820.22320294819772793No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15520.2189702753494777No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15500.21868809715959434No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15360.21671284983041092No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15350.21657176073546924No Hit
GGATATTGTAACTCTGTTGACAGAAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT15260.21530195888099418No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC15230.21487869159616918No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14990.211492553317569No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGGCAACTATTTAAGTTGGTATCAGCAGAAG14290.20161631667165184No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14060.1983712674879933No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14030.1979480002031683No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13340.1882128526521928No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12710.17932423967086736No Hit
CTATTTAAGTTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAGCCTCCTGA12420.17523265591755882No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC12350.1742450322529671No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTTCTGTCAACAGAGTTACAATATC12210.17226978492378367No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12160.1715643394490753No Hit
TTGTAACTCTGTTGACAGAAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC12080.1704356266895419No Hit
GATCTATCCTATATTGACTTTGCACAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11950.16860146845530016No Hit
GGACCCCACTGTGCAAAGTCAATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAA11740.165638597461525No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11510.1623935482778665No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11180.15773760814479126No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC10760.15181186615724096No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10610.14969552973311587No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10570.14913117335334916No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10220.1441930550303906No Hit
GGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACT10180.14362869865062391No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10090.14235889679614883No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10060.14193562951132382No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10000.14108909494167376No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9520.13431681838447343No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9210.12994305644128154No Hit
GTTACAATATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA9190.1296608782513982No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9070.1279678091120981No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA9020.12726236363738974No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9010.12712127454244807No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9000.1269801854475064No Hit
GTCAATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTG8980.12669800725762304No Hit
GTGCAAAGTCAATATAGGATAGATCAGGAGGCTAGGGGCTTTCCCTGGCT8910.12571038359303133No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8760.12359404716890622No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8690.12260642350431451No Hit
GATCAGGAGGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACTTAAAT8660.12218315621948948No Hit
CTTAAATAGTTGCCAATGGTCTGACTTGCCCGGCAAGTGATGGTGACTCT8660.12218315621948948No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8650.1220420671245478No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8610.1214777107447811No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8600.12133662164983944No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8500.1199257307004227No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8340.11766830518135593No Hit
CTCCTGATCTATCCTATATTGACTTTGCACAGTGGGGTCCCGTCAAGGTT8280.11682177061170587No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTTCTGTCAACAGAGTTACAATATCCCGTGGACGTT8260.11653959242182252No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8240.1162574142319392No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8240.1162574142319392No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8100.11428216690275575No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCA8080.11399998871287241No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8030.11329454323816403No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC8020.11315345414322237No Hit
CCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCG7990.11273018685839735No Hit
GACTTTGCACAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGA7980.11258909776345567No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7930.11188365228874729No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7780.1097673158646222No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7730.10906187038991381No Hit
GGCTAGGGGCTTTCCCTGGCTTCTGCTGATACCAACTTAAATAGTTGCCA7680.10835642491520546No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTTCTGTCAACAGAGTTACAATATCCCGTGGACGTTCGGCCAAGGGA7660.1080742467253221No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG7570.10680444487084703No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7550.1065222666809637No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7550.1065222666809637No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7490.10567573211131365No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7440.10497028663660529No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGGATATTGTAACTCTGTTGACAG7370.10398266297201356No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7340.10355939568718855No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7330.10341830659224688No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7300.10299503930742185No Hit
GTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7130.10059652469341339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.9367053E-674.645670
TAAACGA150.002609545867.2379919
GTACTAT150.00261173967.223751
GACCCTA350.00482004657.5837570
GGTAGCG200.00804365850.5964827
CGATTAA400.00817409150.3857870
GTGGTAT1050.044.815831
GTATCAA12500.038.9897731
TCTATAC350.001534924638.3972973
GTACGTA601.11907764E-437.61305669
AGTACAC451.1702337E-437.3307043
TATCAAC14050.034.441882
GTGGTGA3150.034.12370770
AGGTATC400.00295274733.604752
ACTATTC400.002955814433.5976373
ATCAACG14400.033.5976333
TCAACGC14600.033.1373944
CAACGCA14950.032.5863345
AACGCAG15000.032.4777156
GGTACGT653.5531705E-430.89846468
Produced by FastQC (version 0.11.5)