FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014109
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29240.2883319248719812No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT28140.2774849646339792No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG27660.2727517456210329No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24590.24247886568406354No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT22760.22443346819720564No Hit
ATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCAT22740.22423625073833284No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCG22650.22334877217340543No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT22110.2180239007838408No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT20630.20342980882725623No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA19830.19554111047234568No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19570.19297728350699975No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18450.18193310581012495No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17470.17226945032535948No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16780.16546544799424914No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16720.16487379561763085No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16520.1629016210289032No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA16260.16033779406355728No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16200.15974614168693899No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15380.15166022587315564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14850.1464339632130274No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14810.14603952829528186No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14690.1448562235420453No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14600.14396874497711784No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13950.13755917756375302No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13800.13608004662220727No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC13320.13134682760926095No Hit
GGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA12310.12138734593618636No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11570.11409029995789408No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11320.11162508172198453No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA11100.10945568967438411No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10430.1028489048021465No Hit
GGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA10420.10275029607271013No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10360.10215864369609183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)