FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919330
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25240.27454776848356954No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCACCAG18130.19720883687032947No Hit
TTTTTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC17090.1858962505302775No Hit
GTATCAACGCAGAGTACGGGTAATTTTTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGACTGCAGGCTG16450.17893465893639934No Hit
GTGTACTTCTGTGCGAGAGCAGGGGAGGATCACCTTGATATGAGTGGTTATTACCGACCAAATAGCTTTGACTA15150.16479392601133433No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15070.16392372706209957No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14840.1614219050830496No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14820.1612043553457409No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14400.1566358108622584No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14360.156200711387641No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14090.15326378993397366No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13540.1472811721579846No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC13190.1434740517550825No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12770.13890550727159995No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC12720.13836163292832823No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12610.13716510937313045No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11890.12933331883001753No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11740.12770169580020233No Hit
GTCTACATGGACCTGAGCAGCCTGAGTTCTGAAGACACGGCTGTGTACTTCTGTGCGAGAGCAGGGGAGGATCAC11550.12563497329576975No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11450.12454722460922628No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA11450.12454722460922628No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11350.12345947592268282No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11230.12215417749883066No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT11070.12041377960036115No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11050.12019622986305244No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10790.11736808327803944No Hit
GTAATAACCACTCATATCAAGGTGATCCTCCCCTGCTCTCGCACAGAAGT10680.11617155972284163No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10400.11312586340051994No Hit
ATCCATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATGGGATG10380.11290831366321125No Hit
GAAGTACACAGCCGTGTCTTCAGAACTCAGGCTGCTCAGGTCCATGTAGA10250.11149424037070474No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9870.1073607953618396No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9780.10638182154395048No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9600.10442387390817226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9500.1033361252216288No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9450.10279225087835707No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9320.10137817758585058No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9200.10007287916199842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1001.0547228E-745.79254570
GGTATCA12600.040.0020521
GTATCAA31600.035.349061
GTGGTAT4100.034.05581
GTACGTA901.1482462E-633.7766969
TATCAAC33550.032.662322
TGGTATC4250.031.2290942
GAGTTTA4050.031.1127071
TCAACGC35550.030.7140054
AACGCAG35500.030.6647826
CAACGCA35850.030.4619565
ACGCAGA37350.029.1474977
CGCAGAG37750.028.928778
AGAGTAC37300.028.91294511
CAGAGTA37500.028.75874110
TGGTACG904.043599E-628.74382867
CGAACTA756.624034E-527.30859829
AGTTTAT4700.026.7909162
GCGTAGA5650.026.63030270
CAGGTAT1458.1672624E-1025.8354341
Produced by FastQC (version 0.11.5)