FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306381
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76180.5831376910717471No Hit
GACTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAACCTCTACAAACT68970.5279470537308795No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65020.497710851581583No Hit
GTGTAAAGTCTGTGCCGGACCCACTGCCACTGAACCTGTCAGGGACCCCG61520.47091928005689004No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46590.3566340906672709No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46130.3531129126954541No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42660.3265509832124013No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40300.3084858092700368No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37780.28919587777225786No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37440.28659326796700196No Hit
GGTCTAGTCAGAGCCTCTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTC36510.279474364676155No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAGTCTGTGCCGGACCCACTGCCA35020.26806880994135707No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33780.25857693888689437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33250.25451992948458374No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33110.25344826662359604No Hit
AGCCAGGACGGTCTCCAGAGCTCCTGATCTATTTGGCTTCTAATCGGGCC32520.24893197313800491No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32440.24831959436029766No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31370.2401290282084629No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31320.23974629147239587No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCTGGGT31240.23913391269468864No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCTTG30610.2343114298202439No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29280.22413063264086053No Hit
CTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA29190.22344170651593986No Hit
GTCTCCACTCTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCT28820.22060945466904372No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG28660.21938469711362918No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28120.21525114036410514No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27690.21195960443392853No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCCGGCACAGACTTTACACTGAG27660.2117299623922883No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCTCTGGAGACCGTCCTGGCTTCTGCAGGTA25790.1974156084633809No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25330.1938944304915641No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCCTGGTCCCTTGGCCGAACGTC25240.1932055043666434No Hit
CTACAAACTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT24740.18937813700597297No Hit
GTCAATATACCATGGACTGGGTCCGCCAGACTCCAGGAAAGGGGCTGGAG24380.18662243250629026No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24020.18386672800660755No Hit
GGGCTATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT23520.18003936064593715No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCCTGGTCC23430.17935043452101646No Hit
GTTTATTACTGCTTGCAACCTCTACAAACTCCGACGTTCGGCCAAGGGAC23430.17935043452101646No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23320.17850841370166895No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGA23280.17820222431281532No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23050.17644163532690693No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23050.17644163532690693No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22660.173456288785584No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22620.17315009939673037No Hit
CTCTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTCCAGAGCTCCTGAT22400.17146605775803536No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21860.1673325010085113No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21820.16702631161965767No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21760.1665670275363772No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21740.16641393284195038No Hit
GTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTCCAGAGCTCCTGATCTATTTGGCTT21470.16434715446718837No Hit
CTCCAGAGCTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCCCT21470.16434715446718837No Hit
GCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGACTCAGTCTCCACTCTCC21280.1628927548701336No Hit
GTCCTGGCTTCTGCAGGTACCAATTCAAATAGTTGTTTCCATCACTATGC21250.16266311282849338No Hit
GTTCAGTGGCAGTGGGTCCGGCACAGACTTTACACTGAGAATCAGCAGAG20990.16067288180094474No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20920.1601370503704509No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20200.15462564137108545No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19850.15194648421861617No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19710.15087482135762845No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19630.1502624425799212No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19440.1488080429828664No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19100.1462054331776105No Hit
CAATAATATGTGGCCGTGTCCTCGGCGCTCAGGCTGTTCATTTGCAGATA18990.145363412358263No Hit
GCTCAGCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGACTCAGTCTCCACT18960.1451337703166228No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18830.1441386548028485No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18810.14398556010842165No Hit
CTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAG18810.14398556010842165No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18460.14130640295595237No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17860.13671356212314784No Hit
GTCTCCAGAGCTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGGTC17670.13525916252609307No Hit
GCCACAGTTCGTCTGATTTCCACCCTGGTCCCTTGGCCGAACGTCGGAGT17610.1347998784428126No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGACTCAGTCTC17530.13418749966510538No Hit
GAGTTTGTAGAGGTTGCAAGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAGT17320.13258000537362377No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17070.13066632169328854No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17070.13066632169328854No Hit
CCACTCTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCTGCAG16920.12951811148508743No Hit
GTCCGGCACAGACTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAAC16640.127374785763112No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16360.12523146004113653No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16350.12515491269392312No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCT16310.1248487233050695No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16290.12469562861064268No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAACCTCTACAA16240.12431289187457564No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16230.12423634452736222No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16170.12377706044408177No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAACCTCTACAAAC16140.12354741840244156No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16070.1230115869719477No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15890.12163373472210634No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15720.12033242981947839No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15640.11972005104177112No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15480.11849529348635658No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15460.11834219879192977No Hit
GTATATCTGCAAATGAACAGCCTGAGCGCCGAGGACACGGCCACATATTATTGTGCGAGAGATGAAGGTCGAT15400.11788291470864931No Hit
AGTCAGAGCCTCTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTCCAGAG15370.11765327266700909No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15220.11650506245880796No Hit
GTGCCGGACCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCT15140.1158926836811007No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15050.11520375755618001No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15050.11520375755618001No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15010.11489756816732638No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15000.11482102082011297No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14920.11420864204240569No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14890.11397900000076548No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14830.11351971591748503No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGGGACGGGCAGG14700.1125246004037107No Hit
ATGCAAGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGGGACGGGCAGGGAGAGTGGAGA14690.1124480530564973No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14610.11183567427879003No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14590.11168257958436321No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14440.11053436937616208No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14270.10923306447353413No Hit
GAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAACCTCTACAAACTCCGACGTTCGG14210.10877378039025369No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14180.10854413834861347No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14180.10854413834861347No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14140.10823794895975983No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14130.1081614016125464No Hit
GGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC14080.10777866487647937No Hit
GCTCTGGAGACCGTCCTGGCTTCTGCAGGTACCAATTCAAATAGTTGTTTCCATCACTATGCAAGAGGCTCTGA14060.10762557018205257No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14000.10716628609877211No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13820.10578843384893076No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13620.10425748690466258No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAGTCTGTGCCGGACCCACTGCCA13610.10418093955744917No Hit
ATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGT13290.10173142444662009No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13290.10173142444662009No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13240.10134868771055304No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13190.100965950974486No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13180.10088940362727258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)