FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306381
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72310.5535138677001579No Hit
GACTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAACCTCTACAAACT69270.5302434741472817No Hit
GTGTAAAGTCTGTGCCGGACCCACTGCCACTGAACCTGTCAGGGACCCCG59170.452930653461739No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53070.40623677166155964No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46720.35762920618104516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45400.34752495634887526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41840.3202741007409018No Hit
GGTCTAGTCAGAGCCTCTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTC36790.2816176903981304No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36680.2807756695787829No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35740.2735802189407225No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAGTCTGTGCCGGACCCACTGCCA33360.2553619503039313No Hit
CTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA33140.25367790866523626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32870.2516111302904742No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31260.2392870073891154No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31230.2390573653474752No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCTGGGT30870.2363016608477925No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30460.23316321961204273No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29940.22918275755694548No Hit
AGCCAGGACGGTCTCCAGAGCTCCTGATCTATTTGGCTTCTAATCGGGCC29930.22910621020973207No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29350.22466646407135438No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29260.22397753794643369No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29040.2222934963077387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29040.2222934963077387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28860.22091564405789735No Hit
GGGCTATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT27560.2109644889201542No Hit
GTCTCCACTCTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCT27240.20851497380932515No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG26730.2046110591014413No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCTTG24730.1893015896587596No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCCGGCACAGACTTTACACTGAG24440.18708171658957073No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24430.18700516924235733No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24370.18654588515907689No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCCTGGTCCCTTGGCCGAACGTC23970.18348399127054052No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23600.1806517394236444No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23220.17774294022953488No Hit
CTACAAACTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT23020.1762119932852667No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCTCTGGAGACCGTCCTGGCTTCTGCAGGTA22860.17498723572985217No Hit
CTCTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTCCAGAGCTCCTGAT22700.17376247817443763No Hit
GTTTATTACTGCTTGCAACCTCTACAAACTCCGACGTTCGGCCAAGGGAC22630.17322664674394378No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCCTGGTCC22110.1692461846888465No Hit
GTCAATATACCATGGACTGGGTCCGCCAGACTCCAGGAAAGGGGCTGGAG21990.16832761652228562No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21790.16679666957801745No Hit
CTCCAGAGCTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCCCT21730.166337385494737No Hit
GCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGACTCAGTCTCCACTCTCC21690.16603119610588335No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21640.16564845936981631No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21540.16488298589768222No Hit
GTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTCCAGAGCTCCTGATCTATTTGGCTT21050.1611321658842252No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20680.15829991403732907No Hit
GTCCTGGCTTCTGCAGGTACCAATTCAAATAGTTGTTTCCATCACTATGC20340.1556973042320732No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20260.15508492545436592No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGA20190.15454909402387204No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19660.15049208462156138No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19410.14857840094122618No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19320.14788947481630552No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19310.14781292746909208No Hit
GTTCAGTGGCAGTGGGTCCGGCACAGACTTTACACTGAGAATCAGCAGAG19300.14773638012187867No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18850.1442917494972753No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18810.14398556010842165No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18600.1423780658169401No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18530.14184223438644622No Hit
CTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAG18390.1407705715254585No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18380.1406940241782451No Hit
GCTCAGCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGACTCAGTCTCCACT18370.14061747683103168No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18310.14015819274775124No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17950.13740248824806853No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17890.13694320416478808No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17600.1347233310955992No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17460.1336516682346115No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17050.13051322699886175No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17030.1303601323044349No Hit
GAGTTTGTAGAGGTTGCAAGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAGT17010.1302070376100081No Hit
GGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16860.129058827401807No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16770.1283699012768863No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16570.12683895433261813No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16570.12683895433261813No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16540.12660931229097788No Hit
GTCCGGCACAGACTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAAC16510.1263796702493377No Hit
CAATAATATGTGGCCGTGTCCTCGGCGCTCAGGCTGTTCATTTGCAGATA16510.1263796702493377No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGACTCAGTCTC16240.12431289187457564No Hit
GTCTCCAGAGCTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGGTC16180.12385360779129519No Hit
GCCACAGTTCGTCTGATTTCCACCCTGGTCCCTTGGCCGAACGTCGGAGT16000.12247575554145383No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15990.12239920819424041No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15970.12224611349981361No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15950.12209301880538678No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAACCTCTACAAAC15780.12079171390275885No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCTTGCAACCTCTACAA15680.12002624043062476No Hit
CCACTCTCCCTGCCCGTCCCCCCTGGAGAGCCGGCCTCCATCTCCTGCAG15620.11956695634734432No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15520.11880148287521021No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15120.11573958898667387No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15030.11505066286175318No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTGATGCTCT14990.11474447347289957No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14980.11466792612568615No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14970.11459137877847274No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14600.11175912693157664No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14540.11129984284829618No Hit
AGTCAGAGCCTCTTGCATAGTGATGGAAACAACTATTTGAATTGGTACCTGCAGAAGCCAGGACGGTCTCCAGAG14470.11076401141780232No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14390.11015163264009505No Hit
GTATATCTGCAAATGAACAGCCTGAGCGCCGAGGACACGGCCACATATTATTGTGCGAGAGATGAAGGTCGAT14230.1089268750846805No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14190.10862068569582686No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTGAT13940.10670700201549166No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13900.10640081262663802No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13740.10517605507122348No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13630.10433403425187598No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13570.10387475016859554No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13440.10287963465482122No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13320.10196106648826032No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13190.100965950974486No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13180.10088940362727258No Hit
ATGCAAGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGGGACGGGCAGGGAGAGTGGAGA13170.10081285628005918No Hit
GTGCCGGACCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCT13140.10058321423841897No Hit
GCTGATGCTCTGGGTCTCTGGATCCGGTGGGGCTATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCCCCC13110.10035357219677872No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13090.10020047750235192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA300.00256441167.53400470
CCGTTAA200.007809458350.9785616
GGTATCA10000.041.1195531
GTGGTAT3450.040.385711
TGGTATC3500.039.7935262
CGTTAAC350.001431035938.95127527
CCGGTTG350.001452244738.83485415
TAACCGT852.404704E-937.326753
GTATTAG1100.037.0724751
CGCGGTA852.7994247E-936.7510743
GTTTAAC754.27317E-836.54695533
CCGTGCA1002.8740033E-1035.42621656
GTATCAA24100.034.829151
AATAGAT23300.034.49161570
TAACGGC701.0022359E-634.37336
TATAAGA808.129791E-833.9700852
TATCAAC26700.031.8071922
AACCGTG1001.1566954E-831.77949154
ATGGCAA2607.2759576E-1231.1695470
TATTAGA1209.276846E-1131.1392422
Produced by FastQC (version 0.11.5)