FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623088
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC105450.6496875092416431No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG78840.485740760821348No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT76490.4712621866466883No Hit
GATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATGTGGTGTTCGG67790.4176606567234802No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG65280.402196307285865No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC62100.3826040239346234No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT57230.3525994893684138No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATCGTCAGGGAGG55870.3442203996332916No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC54900.3382441371016236No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCACATGGCGACTGGTACCTCCATATGAG50660.31212109263330146No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT48730.3002301785239001No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG48700.30004534566209595No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT48270.2973960746429029No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG47930.2953013022091224No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG46280.2851354948098932No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC44860.2763867393511627No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC44420.27367585737803496No Hit
GACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCACATGGCGACTG44380.27342941356229605No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCACTGAGGTT43230.26634415385980303No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG42870.26412615951815305No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42150.2596901708348531No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC41240.2540835740267934No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA40750.251064637283992No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT40290.2482305334029948No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC39070.24071399702295873No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG38990.24022110939148092No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38570.2376334493262226No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG38120.2348609563991601No Hit
CATATGGAGGTACCAGTCGCCATGTGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTG37650.2319652415642282No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA37010.228022140512406No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34190.2106478515028144No Hit
CTCCTGCACTGGAACCAGCACTGAGGTTGTGAATTATAATATTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC32630.20103654268899773No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC32480.20011237837997695No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC32140.19801760594619638No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCACTGAGGTTGTGAATTATAATATTGTCTCCTGGTACCAACAGCA31840.19616927732815476No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT31520.19419772680224362No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31130.19179489959878945No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC30960.1907475133818992No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30330.1868660232840117No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA30120.18557219325138255No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC29800.18360064272547144No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACGATCTCTGGGCTCCAGG29330.18070492789053952No Hit
ATATTATAATTCACAACCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGAT28330.1745438324970673No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC28090.173065169602634No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG27480.16930690141261595No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT27200.16758179470244375No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27150.16727373993277012No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG26700.16450124700570765No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT26670.16431641414390347No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC26380.16252969647979654No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC26260.16179036503257987No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG26120.16092781167749376No Hit
TTATAATATTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAAC26080.16068136786175488No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGC26060.16055814595388543No Hit
CTCCATATGAGCAGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATCGTCAGGGAGGCCGTGTTGC25920.1596955925987993No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG25500.157107932533541No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG25240.15550604773123824No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT25200.15525960391549937No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT25060.15439705056041322No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC24120.14860562089054938No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA24080.1483591770748105No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT23980.14774306753546326No Hit
CCACATGGCGACTGGTACCTCCATATGAGCAGCAGTAGTAATCAGCCTCG23750.14632601559496466No Hit
GCCTGGAGCCCAGAGATCGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC23730.1462027936870952No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCA23260.1433070788521633No Hit
CCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGAC23230.1431222459903591No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC23220.1430606350364244No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23070.14213647072740357No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC23000.1417051940498605No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA22980.14158197214199106No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGCA22960.14145875023412163No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC22940.14133552832625218No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG22230.13696115059688693No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG22160.13652987391934387No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC21910.13498960007097582No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC21910.13498960007097582No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT21690.13363415908441192No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA21140.13024555661800222No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20850.1284588389538953No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC20630.1271033979673314No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG20580.1267953431976578No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20220.12457734885600782No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC20050.12352996263911753No Hit
ACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCACATGGCGACTGG20030.1234067407312481No Hit
GGCAAAGCCCCCAAACTCATAATTTATGAGGGCAGTAAGCGGCCCTCAGG19880.12248257642222726No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA19860.12235935451435781No Hit
GCTCCAGGCTGAGGACGAGGCTGATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATGTGGTGTTCGGCGG19670.12118874638959809No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG19540.1203878039884467No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA19360.11927880681762172No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC19260.11866269727827451No Hit
ATAATATTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGCAGTAAGCGGC19100.11767692201531896No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG18850.1161366481669509No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC18820.11595181530514673No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG18600.11459637431858284No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTACTACTGCTGCTCATAT18590.11453476336464813No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT18130.1117006594836509No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC17900.1102836075431523No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17820.10979071991167454No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17730.10923622132626204No Hit
GTTGGTACCAGGAGACAATATTATAATTCACAACCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTC17700.10905138846445787No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT17670.10886655560265371No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA17660.10880494464871898No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC17650.10874333369478427No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC17560.10818883510937177No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT17520.10794239129363287No Hit
GGCTGAGGACGAGGCTGATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATGTGGTGTTCGGCGGCGGGAC17330.10677178316887317No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17310.10664856126100372No Hit
GTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGA17200.10597084076772179No Hit
CCCTGACGATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTACTACTGC17010.10480023264296207No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC16950.10443056691935373No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT16900.10412251214968012No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16860.10387606833394122No Hit
GTGCTGTTGGTACCAGGAGACAATATTATAATTCACAACCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGA16740.10313673688672456No Hit
GGACGAGGCTGATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATG16720.10301351497885514No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16690.10282868211705096No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC16650.10258223830131206No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG16610.10233579448557317No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACAATATTATAA16520.10178129590016068No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG16320.10054907682146624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA200.00704152752.3313446
AGGGGGG2750.049.3812170
TACGTAT605.3047354E-447.6485470
CGTATGC2450.044.9527441
TCGTATG2400.044.34008440
GTGGTAT3000.043.5451551
ACCGTAC2550.042.1098127
GGTATCA11650.041.403121
TCTCGTA2500.040.96693838
CTAGATC2400.040.83458333
CGTACTA2650.040.58968429
TGGTATC3550.040.557952
GACCGTA2650.040.5007526
ATCTCGT2600.039.286437
ACTAGAT2800.038.55952532
ATGCCGT2800.038.40730344
TATACAC3150.038.26733
GTATCAA29850.038.040991
ACGAGAC3050.036.23293322
TCTCCGA3050.036.22735211
Produced by FastQC (version 0.11.5)