FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623088
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC98180.6048963457311003No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG72820.4486509665526453No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT69410.42764163126090515No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCACATGGCGACTGGTACCTCCATATGAG67910.41839998817069685No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67630.41667488146052467No Hit
GACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCACATGGCGACTG67030.4129782242244413No Hit
GATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATGTGGTGTTCGG65210.4017650306083219No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC59790.3683718935757026No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG57090.3517369360133277No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC52640.3243200615123764No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT51440.3169267470402098No Hit
GTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATCGTCAGGGAGG51180.315324862237907No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG48070.2961638555642085No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT47200.29080370257188765No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG45880.28267105665250436No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG44740.27564740790394604No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC44630.27496968741066413No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT42070.25919728320337526No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG41500.25568545882909616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41230.2540219630728586No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCACTGAGGTT39030.24046755320721983No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38770.23886566840491705No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC38000.23412162495194347No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC37920.23362873732046566No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA37710.23233490728783654No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT36960.22771408574273236No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG36590.22543448044714764No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC36000.22179943416499903No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35740.22019754936269628No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC35130.21643928117267824No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34940.21526867304791855No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33560.20676636140492688No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33340.205410920418363No Hit
CATATGGAGGTACCAGTCGCCATGTGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTG32660.2012213755508019No Hit
CTCCTGCACTGGAACCAGCACTGAGGTTGTGAATTATAATATTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC32200.19838727166980472No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT32080.19764794022258803No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCACTGAGGTTGTGAATTATAATATTGTCTCCTGGTACCAACAGCA31350.19315034058535335No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC31190.19216456532239778No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC31030.19117879005944224No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28730.17700827065445618No Hit
ACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCACATGGCGACTGG28330.1745438324970673No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG28130.1733116134183729No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC27700.17066234239917982No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA27550.169738178090159No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG26430.16283775124947014No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC25940.15981881450666877No Hit
ATATTATAATTCACAACCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGAT25750.15864820638190905No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC25380.15636860108632433No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC25270.1556908805930424No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT25240.15550604773123824No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT25160.15501316009976046No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG24450.15063878237039519No Hit
TTATAATATTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAAC24360.1500842837849827No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24360.1500842837849827No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24280.14959139615350492No Hit
CCTTGGTCCCGCCGCCGAACACCACATGGCGACTGGTACCTCCATATGAGCAGCAGTAGTAATCAGCCTCGTCCT24030.14805112230513687No Hit
CTCCATATGAGCAGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATCGTCAGGGAGGCCGTGTTGC23850.1469421251343119No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23770.1464492375028341No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACGATCTCTGGGCTCCAGG23760.1463876265488994No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA23740.14626440464102994No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC23530.1449705746084008No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC23520.14490896365446604No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT23510.14484735270053134No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGC23240.14318385694429386No Hit
GCCTGGAGCCCAGAGATCGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC23200.14293741312855496No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA23190.14287580217462023No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC22910.141150695464448No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCA22900.1410890845105133No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG22490.13856303539918968No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC22340.13763887109016887No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT22150.13646826296540915No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21430.13203227428210917No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC21310.13129294283489248No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21270.1310464990191536No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT21140.13024555661800222No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC21120.1301223347101328No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20920.12889011563143835No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20920.12889011563143835No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGCA20850.1284588389538953No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG20800.12815078418422168No Hit
CCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGAC20720.1276578965527439No Hit
CCACATGGCGACTGGTACCTCCATATGAGCAGCAGTAGTAATCAGCCTCG20550.12661051033585363No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG20440.12593278984257167No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT20280.12494701457961613No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20170.12426929408633419No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG19930.12279063119190085No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19230.11847786441647035No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19160.11804658773892729No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCACATGGCGA18980.11693759056810228No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC18910.11650631389055924No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA18810.11589020435121201No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC18620.1147195962264523No Hit
GTTGGTACCAGGAGACAATATTATAATTCACAACCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTC18610.11465798527251757No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC18530.1141650976410398No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18170.11194710329938981No Hit
ATAATATTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGCAGTAAGCGGC18130.1117006594836509No Hit
GTGCTGTTGGTACCAGGAGACAATATTATAATTCACAACCTCAGTGCTGGTTCCAGTGCAGGAGATGGTGATCGA17910.11034521849708705No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA17690.10898977751052316No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG17670.10886655560265371No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17650.10874333369478427No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC17610.10849688987904536No Hit
GCTCCAGGCTGAGGACGAGGCTGATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATGTGGTGTTCGGCGG17220.10609406267559121No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT17220.10609406267559121No Hit
GGCTGAGGACGAGGCTGATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATGTGGTGTTCGGCGGCGGGAC17210.1060324517216565No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT17170.10578600790591762No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG17090.10529312027443984No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16870.10393767928787595No Hit
GGACGAGGCTGATTACTACTGCTGCTCATATGGAGGTACCAGTCGCCATG16780.10338318070246344No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16640.10252062734737734No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG16610.10233579448557317No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA16590.10221257257770375No Hit
GTGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG16370.10085713159113986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA150.00266423566.891066
GTGGTAT2600.051.492761
GGTATCA12450.045.433571
TGGTATC3150.042.479682
GTATCAA30600.037.4079781
TATCAAC34450.033.0157782
AATGGTA4000.032.96454270
ACGGTTA450.004083014631.45533250
ATCAACG36450.031.1945933
TCAACGC36800.030.8098774
CAACGCA37650.030.1143025
AACGCAG38350.029.5646276
GACAGGT2300.029.1046031
GGTACCG656.2066346E-428.09420858
CGCAGAG40450.027.947078
AGAGTAC40650.027.72301711
CAGAGTA41500.027.40031410
CACGGTT657.653223E-427.10514649
TACCGCG550.008918785526.80633560
CAGCGGT500.00899126.76222
Produced by FastQC (version 0.11.5)