FastQCFastQC Report
Wed 8 May 2024
EGAF00004692002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245697
Total Bases16.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42291.721225737391991No Hit
GTATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCC23470.9552416187417836No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCG14390.5856807368425336No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT14380.5852737314659927No Hit
GAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCG11970.4871854357196058No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA11100.4517759679605367No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC11080.4509619572074547No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG11050.4497409410778317No Hit
GGTATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTC10350.42125056471996No Hit
GTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAG10330.4204365539668779No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC10130.4122964464360574No Hit
GGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCC10070.4098544141768113No Hit
GTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG9680.39398120449171137No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG9180.3736309356646601No Hit
GGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCC8630.3512456399549038No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG8590.34961761844873973No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC8250.3357794356463449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.31542916681929367No Hit
GCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7710.3138011453131296No Hit
CCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGG7650.3113591130538834No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6950.28286873669601176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.2645534947516657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.2494942958196478No Hit
GGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC5760.2344350968876299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.23402809151108886No Hit
GAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTC5730.23321408075800681No Hit
GCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTT5420.22059691408523505No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAA5220.2124568065544146No Hit
GAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCG5130.20879375816554535No Hit
ACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGAC5010.20390969364705308No Hit
TATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCCG4960.20187466676434795No Hit
GGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAAC4850.1973976076223967No Hit
ATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCC4780.19454856998660952No Hit
AGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGAT4710.19169953235082235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.18518744632616596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.18355942482000187No Hit
CATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCC4380.17826835492496856No Hit
TCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCC4320.17582632266572243No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.17501231191264038No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4110.16727920975836091No Hit
CTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAAA4090.16646519900527884No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGG4040.16443017212257374No Hit
GCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC4030.1640231667460327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.1619881398633276No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3940.1603601183571635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA3930.15995311298062248No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.152220010826343Clontech SMART CDS Primer II A (100% over 21bp)
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA3670.14937097319055584No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAAAAA3650.1485569624374738No Hit
ATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGC3540.14407990329552253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA3450.14041685490665332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3420.13919583877703023No Hit
CTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGA3340.13593979576470205No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCT3260.13268375275237387No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC3170.12902070436350463No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA3110.1265786721042585No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG3100.12617166672771746No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.1253576559746354No Hit
GGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGG3010.12250861833884825No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2960.12047359145614313No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA2960.12047359145614313No Hit
GTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGT2960.12047359145614313No Hit
GTGGTATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGG2920.11884556994997905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.11762455382035597No Hit
CGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGG2870.11681054306727393No Hit
CCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTT2850.11599653231419187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2840.11558952693765084No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.11070546241915855No Hit
GGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGA2710.11029845704261752No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA2700.1098914516660765No Hit
GTATCAACGCAGAGTACGGGAGCTCCAATAGCGTATATTAAAGTTGCTGC2660.10826343015991242No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT2650.10785642478337137No Hit
GCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGA2650.10785642478337137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.10704241403028934No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG2600.10582139790066628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2580.10500738714758422No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)