FastQCFastQC Report
Wed 8 May 2024
EGAF00004692006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317983
Total Bases97.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGGTCCAACAATCAGAACTACTTAGCTTGGTACCAGCAGAA111170.843485841623147No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT73370.5566839632984645No Hit
GTATAAAACACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA65090.49386069471305777No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61210.46442177175274646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53280.4042540761147906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49480.37542214125675366No Hit
ATTTTAATACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA47990.3641169878518919No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45990.34894228529503035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43390.32921517197111044No Hit
GTATAAATTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAA42630.323448784999503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT41640.3159373072338566No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA41090.31176426403071966No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40720.3089569440577003No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40520.30743947380201414No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC39560.3001556165747206No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39080.29651368796107386No Hit
GTATTAAAATATTGTTGACAAGTATAAATTGCCACATCCTCAGCCTGCAG38390.2912784155789566No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC38030.2885469691187215No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36340.2757243454581736No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG36320.27557259843260495No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC33330.25288641811009704No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC33190.25182418893111674No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT32860.24932036300923457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32570.24712003113848963No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG32280.24491969926774473No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC31840.2415812647052352No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC30970.23498026909300043No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC30620.2323246961455497No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30300.2298967437364518No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG30210.22921388212139307No Hit
AACCAGGACAGCCTCCTCAGTTGCTCATTTACTGGGCGTCTACCCGGGAA28980.21988144004892324No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28690.2176811081781783No Hit
GTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGTAAATGAGCAACTGAG27280.20698294287559096No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG26410.2003819472633562No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAATTTA26330.19977495916108173No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT26320.19969908564829744No Hit
GGCTGAGGATGTGGCAATTTATACTTGTCAACAATATTTTAATACTCCTC25900.1965123981113565No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA25160.19089775816531776No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTGATTGTTGGACCTG24470.18566248578320055No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24010.1821723041951224No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA23640.179364984222103No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23450.17792338747920117No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC23350.1771646523513581No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22670.17200525348202517No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22620.17162588591810365No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA22550.1710947713286135No Hit
ACTGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTGA22320.16934968053457441No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22220.16859094540673134No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT22150.1680598308172412No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21740.16494901679308457No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT21610.1639626611268886No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21530.16335567302461412No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC21300.16161058223057503No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA21060.15978961792375165No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20920.15872738874477135No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG20420.15493371310555598No Hit
GTAAATGAGCAACTGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA20250.15364386338822275No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19930.1512159109791249No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC19720.14962256721065445No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19400.14719461480155663No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT19370.1469669942632037No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC19270.14620825913536062No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19150.14529777698194893No Hit
CACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAATTTATACTTGTCAAC19130.14514602995638032No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19070.14469078887967446No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19010.14423554780296863No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCAGTTGC18940.14370443321347848No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18930.14362855970069419No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18860.143097445111204No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18590.1410488602660277No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18290.1387726548824985No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18280.13869678136971417No Hit
GCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC17940.13611708193504773No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC17880.13566184085834188No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTT17770.1348272322177145No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCAGTTGCTCATT17470.13255102683418526No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17430.13224753278304804No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC17400.13201991224469511No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTC17310.13133705062963635No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATG17150.13012307442508741No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGAT16870.1279986160671268No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT16690.12663289283700926No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16590.1258741577091662No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT16530.12541891663246035No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGGAGGAGTATTAAAATATTGTTGACA16500.12519129609410742No Hit
CTCCTCAGTTGCTCATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCT16490.12511542258132313No Hit
CCTTGGTCCCTCCGCCGAAAGTGGGAGGAGTATTAAAATATTGTTGACAA16410.12450843447904868No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGT16380.12428081394069575No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16350.12405319340234283No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16190.1228392171977939No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT16030.12162524099324498No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA15840.12018364425034314No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15410.11692108320061792No Hit
CATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG15300.11608647455999054No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15190.11525186591936315No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA15020.11396201620202992No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14950.11343090161253977No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14850.1126721664846967No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14790.11221692540799084No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14780.11214105189520654No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGAGG14600.110775328665089No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14520.11016834056281455No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14510.11009246705003023No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14470.10978897299889301No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGTAAATGAGCA14430.10948547894775577No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA14000.10622291789803054No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT13810.1047813211551287No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC13790.10462957412956009No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT13660.10364321846336409No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13570.10296035684830532No Hit
AATTTATACTTGTCAACAATATTTTAATACTCCTCCCACTTTCGGCGGAG13570.10296035684830532No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13350.10129113956705058No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13340.10121526605426626No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13340.10121526605426626No Hit
GGTCCAACAATCAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAG13320.10106351902869765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)