FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677974
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTTATGATTTATGAGGTCC78130.46562104061207144No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65300.38915978435899484No Hit
GACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACATACGGATTG64670.38540525657727714No Hit
CTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTTGGTTCCTGTGCAGGAGATGGTGATCGA61090.3640700034684685No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG59440.3542367164211126No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTC58430.3482175528345493No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58300.34744280900657576No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG57770.3442842380156069No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAC56070.33415297257287657No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG53100.31645305588763595No Hit
GTCCATAATCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAGGTCTGGCAACACGGCCTCCCTGACCAT52990.3157975034178122No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACAGGAACCAACAGTGACGTT51320.3058450250123065No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC50360.3001238398211176No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT47560.28343704968015No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT45270.2697896391720015No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCATGT43760.26079069163169394No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT43240.2576917163198No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42310.2521493181658357No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG42280.25197053112861106No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC41910.24976549100284032No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41850.24940791692839104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41280.24601096322112265No Hit
ATTATGGACCTCATAAATCATAAGTTTGGGGGCTTTGCCTGGGTACTGTT41070.24475945396055004No Hit
GTCGATCACCATCTCCTGCACAGGAACCAACAGTGACGTTGGTGGTTGTAAATATGTCGCTTGGTACCAACAGTA40870.24356754037905237No Hit
CTCCTGCACAGGAACCAACAGTGACGTTGGTGGTTGTAAATATGTCGCTTGGTACCAACAGTACCCAGGCAAAGC39890.23772716382971368No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36910.21996765146539815No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36610.21817978109315161No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC36530.21770301566055253No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36500.21752422862332788No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36270.21615352800460555No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC35870.21376970084161015No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35800.213352531088086No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA35660.21251819158103763No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35350.21067072552971622No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34140.20345964836165517No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGT33750.2011354168777347No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG32940.19630816687266905No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT32190.19183849094205274No Hit
AATTATTACTGCAGCTCATGTACAACCGGAACCAATCCGTATGTGGTGTTCGGCGGAGGGACCAAACTGACCGTC31880.18999102489073133No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAG30650.18266075636452053No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30100.17938299401540192No Hit
CCACATACGGATTGGTTCCGGTTGTACATGAGCTGCAGTAATAATTAGCC29890.17813148475482932No Hit
GGAACCAACAGTGACGTTGGTGGTTGTAAATATGTCGCTTGGTACCAACA29800.17759512364315538No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29790.17753552796408048No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29640.1766415927779572No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC28950.1725294909217902No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28230.16823860202839852No Hit
GTAATAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG28060.1672254754841255No Hit
GTACTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTTGGTTCCTGTGCAGGAGATGGTGATCGA27780.16555679647002874No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCT27700.16508003103742966No Hit
CCCCTGAGGGCCGATTATGGACCTCATAAATCATAAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAAGCGA27510.16394771313500686No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTTATGATTTATGAGGTCCATAATCGGCCCTCAGGGGTTTCTGATCG27480.1637689260977822No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26900.16031237671143891No Hit
GTTTGGTCCCTCCGCCGAACACCACATACGGATTGGTTCCGGTTGTACATGAGCTGCAGTAATAATTAGCCTCGT26550.15822652794381797No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC26540.1581669322647431No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCC26200.156140679176197No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26000.1549487655946993No Hit
ACAGTACCCAGGCAAAGCCCCCAAACTTATGATTTATGAGGTCCATAATCGGCCCTCAGGGGTTTCTGATCGCTT25820.1538760433713514No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25600.15256493843170396No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC25010.14904879336628576No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG24610.1466649662032904No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC24440.14565183965901737No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24330.14499628718919363No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG24260.14457911743566945No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC24120.14374477792862106No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23800.14183771619822477No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23440.13969227175152893No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23330.1390367192817052No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG23230.13844076249095635No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22960.13683167915593447No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22930.13665289211870982No Hit
GGACCAAACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG22770.13569936125351167No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22690.13522259582091262No Hit
TAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAG22670.13510340446276284No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTC22640.13492461742553818No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC22580.13456704335108885No Hit
GGGTACTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTT22490.13403068223941492No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAGCGATC22350.13319634273236655No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC22310.132957960016067No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22190.13224281186716838No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC21970.13093170692752093No Hit
GTTGGTTCCTGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGA21870.13033575013677207No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21580.12860747544360043No Hit
GTTGTAAATATGTCGCTTGGTACCAACAGTACCCAGGCAAAGCCCCCAAA21480.1280115186528516No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC21470.1279519229737767No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT21170.12616405260153016No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT21040.1253893087735567No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20730.12354184272223526No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC20500.12217114210351292No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA20260.1207408458057157No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG20250.12068125012664081No Hit
GCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCATGTACAACCGGAACCAATCCGTATGTGGTGTTCGG19870.11841661432179522No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT19270.11484087357730216No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC19260.11478127789822726No Hit
GAACCAATCCGTATGTGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTA18810.11209947233985747No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18790.11198028098170769No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG18410.1097156451768621No Hit
GGCTGAGGACGAGGCTAATTATTACTGCAGCTCATGTACAACCGGAACCA18310.10911968838611324No Hit
GGCTAATTATTACTGCAGCTCATGTACAACCGGAACCAATCCGTATGTGGTGTTCGGCGGAGGGACCAAACTGAC18240.10870251863258906No Hit
CTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC17810.10613990443236904No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA17590.10482879949272159No Hit
GGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAGCGATCAGAAA17560.10465001245549693No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17530.10447122541827228No Hit
GATTGGTTCCGGTTGTACATGAGCTGCAGTAATAATTAGCCTCGTCCTCA17230.10268335504602574No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC17150.10220658961342668No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA17060.10167022850175271No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA17030.10149144146452806No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA17020.10143184578545318No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17010.10137225010637828No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG16910.10077629331562944No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT16810.10018033652488059No Hit
GTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTTGGTTC16800.10012074084580572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)