FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677974
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105180.6268273525096336No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTTATGATTTATGAGGTCC75900.4523312041783722No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG63470.378253775088291No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG63220.3767638831114189No Hit
GACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACATACGGATTG62710.3737245034785998No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61410.3659770651988648No Hit
CTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTTGGTTCCTGTGCAGGAGATGGTGATCGA57370.3419004108526115No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57210.3409468799874134No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAC54970.3275974478746393No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACAGGAACCAACAGTGACGTT54250.32330655898124766No Hit
GTCCATAATCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAGGTCTGGCAACACGGCCTCCCTGACCAT53390.3181813305808076No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTC50730.30232887994688834No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC50080.29845516080702084No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG50080.29845516080702084No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG46810.2789673737495337No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG43910.2616846268178172No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCATGT42920.25578465458940364No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC42000.2503018521145143No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT41530.24750085519799472No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40150.23927665148566069No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC39780.23707161135988997No Hit
ATTATGGACCTCATAAATCATAAGTTTGGGGGCTTTGCCTGGGTACTGTT39170.23343627493632202No Hit
GTCGATCACCATCTCCTGCACAGGAACCAACAGTGACGTTGGTGGTTGTAAATATGTCGCTTGGTACCAACAGTA39100.23301910518279784No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38930.2320059786385248No Hit
CTCCTGCACAGGAACCAACAGTGACGTTGGTGGTTGTAAATATGTCGCTTGGTACCAACAGTACCCAGGCAAAGC38570.22986053419182897No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37720.2247949014704638No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT37260.22205350023301912No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35970.21436565763235904No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35700.21275657429733713No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34970.2084060897248706No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34620.20632024095724963No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33970.20244652181738215No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC32990.19660614526804346No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32470.1935071699561495No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32120.19142132118852856No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC31910.19016981192795598No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT31590.18826275019755967No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31580.1882031545184848No Hit
AATTATTACTGCAGCTCATGTACAACCGGAACCAATCCGTATGTGGTGTTCGGCGGAGGGACCAAACTGACCGTC31550.18802436748126014No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31330.18671326254161266No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31100.18534256192289036No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC30960.18450822241584194No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGT30110.1794425896944768No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28990.17276787363808976No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAG28760.1713971730193674No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC28690.17098000326584323No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC28690.17098000326584323No Hit
GTTTGGTCCCTCCGCCGAACACCACATACGGATTGGTTCCGGTTGTACATGAGCTGCAGTAATAATTAGCCTCGT28280.16853658042377295No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27430.16347094770240778No Hit
CCACATACGGATTGGTTCCGGTTGTACATGAGCTGCAGTAATAATTAGCC27210.16215984276276033No Hit
GGAACCAACAGTGACGTTGGTGGTTGTAAATATGTCGCTTGGTACCAACA26560.15828612362289285No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26180.15602148781804726No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCT25900.15435280880395047No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG25620.1526841297898537No Hit
GTACTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTTGGTTCCTGTGCAGGAGATGGTGATCGA25590.15250534275262906No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25190.1501215155896337No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC25040.14922758040351042No Hit
CCCCTGAGGGCCGATTATGGACCTCATAAATCATAAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAAGCGA25010.14904879336628576No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCC24980.1488700063290611No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTTATGATTTATGAGGTCCATAATCGGCCCTCAGGGGTTTCTGATCG24910.14845283657553693No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24620.14672456188236527No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC24500.14600941373346668No Hit
GTAATAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG24260.14457911743566945No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23240.13850035817003123No Hit
GTTGTAAATATGTCGCTTGGTACCAACAGTACCCAGGCAAAGCCCCCAAA23130.13784480570020752No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA23010.1371296575513089No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC22990.13701046619315913No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22800.13587814829073633No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22540.13432866063478932No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTC22460.13385189520219026No Hit
ACAGTACCCAGGCAAAGCCCCCAAACTTATGATTTATGAGGTCCATAATCGGCCCTCAGGGGTTTCTGATCGCTT22380.13337512976959118No Hit
GTTGGTTCCTGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGA22330.13307715137421677No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG22300.13289836433699212No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT22290.13283876865791722No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG21710.12938221927157395No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21660.12908424087619952No Hit
GGACCAAACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21430.12771354025747716No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC21340.1271771791458032No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20920.12467416062465807No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC20900.12455496926650829No Hit
GGGTACTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTT20570.12258831185703713No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT20090.11972771926144267No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA20070.1196085279032929No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20060.119548932224218No Hit
TAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAG20050.11948933654514313No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19980.11907216679161894No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19690.11734389209844728No Hit
GGCTGAGGACGAGGCTAATTATTACTGCAGCTCATGTACAACCGGAACCA19520.11633076555417425No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT19480.11609238283787472No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT19180.11430451246562819No Hit
GCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCATGTACAACCGGAACCAATCCGTATGTGGTGTTCGG18980.1131125988841305No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18920.11275502480968119No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG18590.11078836740021No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC18590.11078836740021No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAGCGATC18290.1090004970279635No Hit
GAACCAATCCGTATGTGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTA18260.10882170999073884No Hit
CTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC18080.1077489877673909No Hit
GTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAAGCGACATATTTACAACCACCAACGTCACTGTTGGTTC18050.10757020073016625No Hit
GGCTAATTATTACTGCAGCTCATGTACAACCGGAACCAATCCGTATGTGGTGTTCGGCGGAGGGACCAAACTGAC18000.10727222233479185No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17960.1070338396184923No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17820.10619950011144391No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC17650.10518637356717088No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17520.1044116297391974No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG17440.10393486430659832No Hit
CCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG17350.10339850319492434No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA17260.1028621420832504No Hit
GGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAGCGATCAGAAA17190.10244497232972621No Hit
CTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC17180.10238537665065131No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC17100.10190861121805225No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC17050.10161063282267783No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16910.10077629331562944No Hit
GGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGT16870.1005379105993299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG150.002743285666.3995817
TAGTATC200.00840213150.044427
GATTGAC1600.033.2225721
TTTAAGT4450.033.0752870
GTATCAA62650.030.4385591
TCCAACG605.094375E-429.05886844
GGTATCA30200.028.6022151
CAGGTAT1303.074092E-1028.1114061
TATCAAC67900.028.070822
GCTTGTA606.762286E-427.6854761
AACGCAG71050.026.7699876
GACAGCG1006.9796624E-726.5780581
GACAGGT1750.026.5780561
ATCAACG71100.026.5699633
GTTACTT500.00934090526.5534979
CAACGCA71950.026.3452175
TCAACGC71700.026.3444774
TGCAGTA39700.026.32275670
ATGGCAA3657.2759576E-1226.21102770
CGCGCTA659.915185E-425.92950633
Produced by FastQC (version 0.11.5)