FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249562
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA107310.8587809168332584No Hit
ATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCAT104390.8354127286201085No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75230.6020509586559131No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC72550.5806034434465837No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC67260.538268609320706No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA57530.4604013246241483No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53670.429510500479368No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50070.4007004054220599No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49010.3922174329885192No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47330.3787727219617754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46990.37605176853969635No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46260.37020972148640885No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43950.35172324382463616No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCT43790.3504427951554225No Hit
ACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT42290.3384385888815441No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG40640.3252339619802779No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36940.29562358650471127No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36520.2922624087480253No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35990.28802092253125494No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35330.2827390717707485No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32620.26105147243594157No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAA32470.2598510518085537No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31720.25384894867161456No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31620.253048668253356No Hit
CTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC30060.24056429372852245No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC29770.23824348051557265No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28780.23032070437481295No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28230.2259191620743909No Hit
GTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTC28180.22551902186526157No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA26780.21431509600964177No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26150.20927332937461288No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25790.20639231986888207No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24920.19942988023003258No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA24850.19886968393725163No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24690.19758923526803793No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24110.1929476088421383No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23660.18934634695997477No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT23570.18862609458354207No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC23320.18662539353789567No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG23050.18446463640859756No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22740.18198376711199604No Hit
GTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTA22490.17998306606634965No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22470.17982300998269793No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22170.17742216872792227No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21950.17566155180775345No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21850.1748612713894949No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21250.17005958887994355No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20950.16765874762516786No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20860.16693849524873514No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20800.16645832699778002No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20680.16549799049586977No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20340.16277703707379065No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20190.1615766164464028No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20180.16149658840457695No Hit
TCTTAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCA20010.16013611169353742No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19650.1572551021878066No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19590.15677493393685146No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19400.15525440114216021No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19120.15301361597103624No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19120.15301361597103624No Hit
CTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATC19110.15293358792921039No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19080.15269350380373284No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18580.14869210171244004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18170.14541095199757995No Hit
GGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTT17880.14309013878463014No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17870.1430101107428043No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA17830.14268999857550085No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17740.14196974619906816No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTT17720.14180969011541644No Hit
ACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATT17660.1413295218644613No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17300.1384485123587305No Hit
AGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA17290.13836848431690463No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17070.13660786739673583No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16840.13476722243474112No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16730.1338869139746567No Hit
ATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGA16680.13348677376552742No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCC16630.13308663355639816No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16620.1330066055145723No Hit
AATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA16560.13252643726361718No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT16250.13004556796701564No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16190.12956539971606051No Hit
GAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGC16120.1290052034232795No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16090.12876511929780196No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16070.12860506321415022No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16000.12804486692136924No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15750.12604416587572287No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15710.12572405370841944No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15630.12508382937381257No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15570.12460366112285745No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15460.12372335266277303No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15150.12124248336617151No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15120.12100239924069393No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15100.12084234315704223No Hit
CCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15060.12052223098973881No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14890.11916175427869925No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTA14750.11804136169313727No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTAC14690.11756119344218215No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTA14630.11708102519122701No Hit
GCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTC14460.11572054848018745No Hit
CAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA14390.11516035218740647No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG14320.11460015589462548No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCA14300.11444009981097378No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14250.11403995960184449No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG14240.11395993156001862No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14130.11307962309993422No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGT13610.10891816492498972No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13440.10755768821395016No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT13280.10627723954473647No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13170.10539693108465208No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13170.10539693108465208No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA13130.10507681891734864No Hit
ACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC12950.10363631416448324No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC12720.10179566920248855No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT12690.10155558507701097No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG12650.10123547290970757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3250.058.4455262
CCAACCG308.459732E-658.2406348
GTGGTAT3850.051.1237221
GGTATCA17150.047.8857731
TCGTATG3000.045.96146840
CGTATGC2950.045.64436341
CCGAAAT404.4322267E-544.01804452
GCCGTCT3000.042.9246346
TCTCCGA3650.042.7424511
TCCGAGC3700.042.16822413
CGTCTTC2950.041.45943548
ACGAGAC3700.041.27134322
ATGCCGT3300.040.98528344
CCGTCTT3150.040.95796647
CTTATAC3900.040.896721
GTATCAA47650.040.879371
ATCTCGT3050.040.5010637
CACGAGA3700.040.34935421
CCCACGA3750.039.8002119
CGAGACA4050.039.38675723
Produced by FastQC (version 0.11.5)