FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729508
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC111640.6455014952229189No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGA96610.5585981678026353No Hit
CTCCTGCACTGGGAGCAGCTCCAACATTGGGGCAATTTATGCTGTTCAGTGGTACCAACAACTTCCAGGAACAGC70760.40913369582563364No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT64700.37409482928092846No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58910.3406171003545517No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC53740.3107242059591514No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC53270.3080066701050241No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT49360.28539908459515656No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC48670.2814095106816505No Hit
GTTCAGTGGTACCAACAACTTCCAGGAACAGCCCCCAAACCCCTCATTTA47270.2733147230310585No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT42430.24532988572472633No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42180.2438843879299778No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42080.24330618881207833No Hit
GATTATTACTGCCACTGCTATGACAACATCCTGAGTGGTTCGGTATTCGG41970.24267016978238898No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG40370.2334189838959982No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40270.23284078477809875No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACCAGAAAATCGGTC40110.23191566618945966No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG39560.22873557104101283No Hit
GGCCATCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCACT39060.2258445754515157No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA37240.21532135150574613No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC36570.21144741741582No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36530.21121613776866022No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35320.20421992844207718No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35310.20416210853028724No Hit
GTAATAATCAGCCTCATCTTCAGGCTGGAGCCCAGTGATGGCCAGGGAGG34830.20138675276437No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34750.20092419347005042No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT34450.19918959611635217No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGA34330.19849575717487286No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG33800.19543130185000593No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT33120.19149954784828982No Hit
TCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCACTGCTAT31940.18467679825707659No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG31800.18386731949201737No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31550.1824218216972688No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGG31550.1824218216972688No Hit
GGATGTTGTCATAGCAGTGGCAGTAATAATCAGCCTCATCTTCAGGCTGG30340.17542561237068577No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCAATTTATGCTGTTCAGTGGTACCAA29740.17195641766328923No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29620.17126257872180992No Hit
ACTCAGGATGTTGTCATAGCAGTGGCAGTAATAATCAGCCTCATCTTCAG29120.16837158313231276No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC27730.16033461539351077No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27450.15871565786339237No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAC27430.15860001803981247No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26890.15547774280315557No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT26590.1537431454494573No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCAATTTATG26390.15258674721365845No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26160.15125688924248978No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC25850.14946447197700155No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC25840.14940665206521161No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25040.14478105912201622No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT25020.14466541929843632No Hit
CATTTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGTTCCAAGTCTGGCACCTCAGCCTC24650.14252608256220844No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24370.14090712503209005No Hit
ATACCGAACCACTCAGGATGTTGTCATAGCAGTGGCAGTAATAATCAGCC24100.13934598741376159No Hit
GCCGAATACCGAACCACTCAGGATGTTGTCATAGCAGTGGCAGTAATAAT23830.13778484979543315No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23820.13772702988364321No Hit
CCCCTGAGGGCCGATTGTTGTTACCATAAATGAGGGGTTTGGGGGCTGTTCCTGGAAGTTGTTGGTACCACTGAA22940.13263887764612825No Hit
GCATAAATTGCCCCAATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA22870.13223413826359864No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22740.13148247941032942No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT22710.1313090196749596No Hit
CCACTGAACAGCATAAATTGCCCCAATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGC22590.13061518073348027No Hit
GCCCTCAGGGGTCCCTGACCGATTTTCTGGTTCCAAGTCTGGCACCTCAG22510.13015262143916073No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22270.12876494355620213No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22190.12830238426188256No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG22100.12778200505577308No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC21940.126856886467134No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGCCTGAAGATG21500.12431281034837655No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT21490.1242549904365866No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21230.12275167273004808No Hit
GCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGATGTTGTCATAGCAGTGGCAGTAATAATCAGCCTCATCTT21210.12263603290646818No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21200.12257821299467826No Hit
GCCCCCAAACCCCTCATTTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGTTCCAAGT21030.12159527449424924No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT20790.12020759661129062No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20670.1195137576698113No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20550.118819918728332No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG20360.11772134040432308No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG20360.11772134040432308No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC20240.11702750146284377No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG20160.11656494216852423No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACCAGAAAAT20150.11650712225673428No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20130.1163914824331544No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20030.11581328331525496No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20020.11575546340346504No Hit
GACAACATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC20000.11563982357988514No Hit
GCTCCAACATTGGGGCAATTTATGCTGTTCAGTGGTACCAACAACTTCCA19920.1151772642855656No Hit
GCTATGACAACATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTG19860.11483034481482594No Hit
AATTTATGCTGTTCAGTGGTACCAACAACTTCCAGGAACAGCCCCCAAAC19670.11373176649081705No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGTTGGTACCACTGAACAGCATAAATT19380.11205498904890869No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC19360.11193934922532883No Hit
ATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGG19270.11141897001921933No Hit
GTGCCAGACTTGGAACCAGAAAATCGGTCAGGGACCCCTGAGGGCCGATT19270.11141897001921933No Hit
GTCATAGCAGTGGCAGTAATAATCAGCCTCATCTTCAGGCTGGAGCCCAG19090.11037821160700037No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18950.10956873284194119No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC18860.10904835363583168No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA18730.10829669478256244No Hit
GTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC18720.10823887487077251No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT18660.10789195540003285No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18330.10598389831096473No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18080.10453840051621618No Hit
CCTCAGGGGTCCCTGACCGATTTTCTGGTTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGC18010.10413366113368658No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT17940.10372892175115697No Hit
CATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT17710.10239906377998831No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17570.1015895850149291No Hit
ATTTATGCTGTTCAGTGGTACCAACAACTTCCAGGAACAGCCCCCAAACCCCTCATTTATGGTAACAACAATCGG17310.1000862673083906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3300.049.07881540
CGTATGC3400.047.7510241
TCTCGTA3350.047.09084338
GGTATCA15100.044.2339061
ACGAGAC3700.042.8375922
ATGCCGT3750.042.6726644
CGAGACG3850.041.17335523
ATCTCGT3850.040.8933837
GCCGTCT3900.040.33432846
AGGGGGG4900.039.7471670
GTGGTAT4000.039.637381
TATACAC4250.038.8752563
CCCACGA4200.037.7335119
TCTATAC451.1464776E-437.4648133
TCTCCGA4350.037.20428511
AGACGGA4300.036.87838425
CGAGCCC4400.036.78363815
CCGTCTT4300.036.68015347
TACATCT4400.036.35790334
TGGTATC4400.036.017312
Produced by FastQC (version 0.11.5)