FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729508
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC103800.6001706843796039No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGA83910.4851668798294081No Hit
CTCCTGCACTGGGAGCAGCTCCAACATTGGGGCAATTTATGCTGTTCAGTGGTACCAACAACTTCCAGGAACAGC70350.406763079442246No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG61910.3579630738915345No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61170.3536844004190787No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG57980.3352398485580871No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC54980.3178938750211043No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47440.27429766153148755No Hit
GTTCAGTGGTACCAACAACTTCCAGGAACAGCCCCCAAACCCCTCATTTA46440.2685156703524933No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT45680.2641213570564577No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC45670.26406353714466774No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC45410.26256021943812924No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG44320.25625784905302545No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41750.24139813172301022No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40170.23226258566019933No Hit
GGCCATCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCACT39630.2291403104235424No Hit
GATTATTACTGCCACTGCTATGACAACATCCTGAGTGGTTCGGTATTCGG39570.22879339095280277No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37370.21607301035901538No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT37210.21514789177037633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG37010.21399149353457744No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC36580.21150523732760992No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36040.20838296209095306No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35670.20624362535472518No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34570.1998834350578315No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA34510.19953651558709184No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34060.1969346195565444No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG33990.19652988017401482No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT33090.19132608811291998No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC32550.18820381287626306No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACCAGAAAATCGGTC32220.18629575578719498No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC32200.18618011596361508No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG31250.18068722434357054No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30820.17820096813660302No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30100.17403793448772714No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29690.1716673181043395No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCAATTTATGCTGTTCAGTGGTACCAA29280.16929670172095185No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC28780.16640570613145472No Hit
TCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCACTGCTAT28740.16617442648429495No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC28190.16299433133584812No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28020.1620113928354191No Hit
GTAATAATCAGCCTCATCTTCAGGCTGGAGCCCAGTGATGGCCAGGGAGG27620.1596985963638214No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27450.15871565786339237No Hit
GGATGTTGTCATAGCAGTGGCAGTAATAATCAGCCTCATCTTCAGGCTGG27330.15802181892191305No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGA26960.15588248218568518No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCAATTTATG26750.1546682640380964No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGG26110.15096778968354005No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT25870.14958011180058142No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25080.14501233876917596No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT24460.14142750423819953No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG24390.14102276485566995No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAC24060.13911470776660184No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23590.13639717191247452No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23430.13547205332383544No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT23390.1352407736766757No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC23380.13518295376488573No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23250.13443129491161648No Hit
ACTCAGGATGTTGTCATAGCAGTGGCAGTAATAATCAGCCTCATCTTCAG22700.13125119976316962No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22400.12951660240947135No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC22360.12928532276231158No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGCCTGAAGATG22260.12870712364441217No Hit
CATTTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGTTCCAAGTCTGGCACCTCAGCCTC22080.1276663652321932No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21380.12361897140689723No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC21350.1234455116715274No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG21140.1222312935239386No Hit
GCCCTCAGGGGTCCCTGACCGATTTTCTGGTTCCAAGTCTGGCACCTCAG20810.12032323643487049No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20580.11899337846370181No Hit
GCCCCCAAACCCCTCATTTATGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGTTCCAAGT20330.11754788066895325No Hit
GTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC20270.11720096119821359No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC20070.11604456296241475No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT20040.11587110322704491No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG20020.11575546340346504No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19970.11546636384451531No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC19820.11459906516766619No Hit
GCCGAATACCGAACCACTCAGGATGTTGTCATAGCAGTGGCAGTAATAAT19620.11344266693186732No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19510.11280664790217795No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC19420.11228626869606848No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19370.11199716913711877No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC19340.11182370940174893No Hit
GACAACATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC19230.11118769037205957No Hit
ATACCGAACCACTCAGGATGTTGTCATAGCAGTGGCAGTAATAATCAGCC19160.11078295098952996No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA19100.1104360315187903No Hit
GCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGATGTTGTCATAGCAGTGGCAGTAATAATCAGCCTCATCTT18890.10922181337120153No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18880.10916399345941158No Hit
AATTTATGCTGTTCAGTGGTACCAACAACTTCCAGGAACAGCCCCCAAAC18800.10870143416509204No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18610.10760285584108314No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG18580.1074293961057133No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG18410.10644645760528428No Hit
GCATAAATTGCCCCAATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA18390.10633081778170439No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18380.10627299786991444No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18140.10488531998695584No Hit
GCTATGACAACATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTG18130.10482750007516589No Hit
ATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGG18090.1045962204280061No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC18090.1045962204280061No Hit
CCCCTGAGGGCCGATTGTTGTTACCATAAATGAGGGGTTTGGGGGCTGTTCCTGGAAGTTGTTGGTACCACTGAA17910.10355546201578714No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17820.10303508280967767No Hit
CCACTGAACAGCATAAATTGCCCCAATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGC17660.10210996422103859No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCAATTTATGCTGTTCAGTGGTACCAACAACTTC17540.10141612527955927No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGTTGGTACCACTGAACAGCATAAATT17500.10118484563239949No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC17500.10118484563239949No Hit
CATCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT17430.10078010624986991No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17370.10043318677913025No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT17370.10043318677913025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5400.042.4585231
GGTATCA15950.040.5873151
TGGTATC5650.040.5528262
GTATCAA39900.036.4211921
TACGTAT951.4261226E-436.09784370
CGACCTA400.00272779134.15515536
TATCAAC45250.031.6468892
AACTAAG3700.031.5124470
ATCAACG46700.030.6616133
TCAACGC47300.030.34394
CAACGCA48050.030.0105065
AACGCAG48950.029.3210746
CAACCGA854.1456897E-628.6547344
CGCAGAG51050.028.1141038
ACGCAGA51450.027.9610127
AGAGTAC51900.027.6544611
CAGAGTA52250.027.46921510
GCAGAGT52650.027.2597339
CAACTAA3250.027.06502369
CGGTCTA500.00868077226.95570420
Produced by FastQC (version 0.11.5)