FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730644
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26500.36269373319975257No Hit
ATTTTAAACAGTGAAGCCCCATCGCGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGC25910.3546186651775694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25760.35256568178209907No Hit
GTATGATACTTATTCCGCCTTTGGCCAGGGGACCAAAGTAGAGATGCAACGAACTGTGGCTGCACCATCTGTCTT24730.338468529133203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22810.3121903416711832No Hit
GTATCATACTGTTGGCAGTAATAAGATGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22370.30616825704447037No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19680.269351421485703No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19380.2652454546947624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18940.2592233700680496No Hit
GTTTAAAATAGACGCCTTGTAGATCAGGAGGTTAGGGGCTTTCCCTGGTT18250.2497796464488862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16980.2323977203672377No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16410.22459638346445054No Hit
GAATATTAGTAGTTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTACAAGGCGT16240.22226966894958422No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15640.21405773536770303No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15340.2099517685767624No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15230.2084462474200842No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14880.20365595283065352No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14540.19900252380092082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14270.1953071536890743No Hit
CCTTCCACCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAGTAGTTGG14190.19421222921149014No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGCATCTCTACTTTGGTCC13920.1905168590996436No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13690.18736895122658914No Hit
GTGAAGCCCCATCGCGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT13220.1809362699207822No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12840.17573537865225747No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12570.17204000854041093No Hit
TCTTATTACTGCCAACAGTATGATACTTATTCCGCCTTTGGCCAGGGGAC12470.17067135294343072No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12230.16738657951067826No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11890.16273315048094558No Hit
AAGTAGAGATGCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11860.1623225538018515No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCATCTTA11480.15712166253332677No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11310.15479494801846042No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11290.15452121689906437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11160.15274196462299014No Hit
TCTCTACTTTGGTCCCCTGGCCAAAGGCGGAATAAGTATCATACTGTTGG10980.15027838454842576No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10890.1490465945111436No Hit
CTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA10770.14740420779476734No Hit
GTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10550.14439316548141093No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10470.14329824100382677No Hit
TACTTATTCCGCCTTTGGCCAGGGGACCAAAGTAGAGATGCAACGAACTGTGGCTGCACCATCTGTCTTCATCTT10230.1400134675710743No Hit
GTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCC10130.1386448119740941No Hit
GATCTACAAGGCGTCTATTTTAAACAGTGAAGCCCCATCGCGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA10050.13754988749650993No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10010.13700242525771783No Hit
GAATAAGTATCATACTGTTGGCAGTAATAAGATGCAAAATCATCAGGCTG10010.13700242525771783No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9900.13549690410103965No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9840.1346757107428515No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9570.130980340631005No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT9540.13056974395191093No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCATCTTATTACTGCCAACAGTATGATACTTATTCCGCCTTTGG9390.12851676055644062No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9360.12810616387734655No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9340.1278324327579505No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTT9340.1278324327579505No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9240.12646377716097032No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9180.1256425838027822No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCACC9150.12523198712368816No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA9140.12509512156399014No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9130.12495825600429211No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8980.1229052726088218No Hit
GTAATAAGATGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG8900.12181034813123766No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8860.12126288589244558No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8720.11934676805667328No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8680.1187993058178812No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8630.11811497801939111No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8580.11743065022090102No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8570.11729378466120301No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8560.11715691910150498No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8530.11674632242241093No Hit
CTACTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAGACAGGGTGGAAGGAGA8470.1159251290642228No Hit
GTGCAGCCACAGTTCGTTGCATCTCTACTTTGGTCCCCTGGCCAAAGGCG8400.11496707014633666No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8400.11496707014633666No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8350.11428274234784655No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8300.11359841454935644No Hit
CAGTATGATACTTATTCCGCCTTTGGCCAGGGGACCAAAGTAGAGATGCAACGAACTGTGGCTGCACCAT8290.11346154898965843No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8250.11291408675086637No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8210.11236662451207428No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCGCGATGGGGCTTCA8200.11222975895237627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.11209289339267825No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8170.1118191622732822No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTACAAGGCGTCTATTTTAAACAGTGAAGCCCCAT8160.11168229671358418No Hit
GGCCAAAGGCGGAATAAGTATCATACTGTTGGCAGTAATAAGATGCAAAA8160.11168229671358418No Hit
GCCCCTAACCTCCTGATCTACAAGGCGTCTATTTTAAACAGTGAAGCCCCATCGCGGTTCAGCGGCAGTGGAT8090.11072423779569804No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT8040.11003990999720795No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7910.10826065772113369No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7900.10812379216143568No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAGCCAACTACTA7790.10661827100475745No Hit
CACCATCACTTGCCGGGCCAGTCAGAATATTAGTAGTTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC7590.10388095981079704No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7540.10319663201230696No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT7380.10100678305713863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG200.005014831757.0155262
CGTATGC1200.055.09274341
TCGTATG1250.049.860340
CCTAGGT200.00882704349.4207343
TAAGGCG1400.045.21852529
GCGAATC1300.044.07120533
ATCTCCG1450.043.16327310
GACTAAG1550.042.69071626
GGCGAAT1500.042.554532
CCCACGA1550.042.5270119
ACTAAGG1500.041.98019427
GTATGCC1700.041.07832342
AGACTAA1550.040.49772325
CGAGACT1550.040.43388723
GAGACTA1650.038.01200524
ACGAGAC1650.037.9755422
GCCGTCT1700.037.50097346
CTAAGGC1700.037.13339228
TCCGAGC1800.036.60293613
TCTCCGA1800.036.60043311
Produced by FastQC (version 0.11.5)