FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730644
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26130.3576297074909258No Hit
GTATGATACTTATTCCGCCTTTGGCCAGGGGACCAAAGTAGAGATGCAACGAACTGTGGCTGCACCATCTGTCTT24970.34175330256595554No Hit
ATTTTAAACAGTGAAGCCCCATCGCGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGC24690.3379210668944109No Hit
GTATCATACTGTTGGCAGTAATAAGATGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC23350.3195810818948763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22510.30808437488024265No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20650.2826273807764109No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20160.275920968351208No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18780.25703352111288125No Hit
GTTTAAAATAGACGCCTTGTAGATCAGGAGGTTAGGGGCTTTCCCTGGTT18460.25265382320254465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17620.24115711618791094No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16100.2203535511138119No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15540.21268907977072282No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15490.2120047519722327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15190.2078987851812921No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14630.200234313838203No Hit
GAATATTAGTAGTTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTACAAGGCGT14460.19790759932333668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14070.19256984249511389No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13910.19037999353994559No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13910.19037999353994559No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGCATCTCTACTTTGGTCC13570.1857265645102129No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13560.1855896989505149No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13120.17956761432380203No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12490.17094508406282677No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12100.165607327234604No Hit
CCTTCCACCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAGTAGTTGG11890.16273315048094558No Hit
TCTTATTACTGCCAACAGTATGATACTTATTCCGCCTTTGGCCAGGGGAC11620.15903778036909905No Hit
GTGAAGCCCCATCGCGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT11600.158764049249703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11120.15219450238419804No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11120.15219450238419804No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11010.15068898122751984No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10850.1484991322723515No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10830.1482254011529555No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCATCTTA10780.14754107335446537No Hit
TACTTATTCCGCCTTTGGCCAGGGGACCAAAGTAGAGATGCAACGAACTGTGGCTGCACCATCTGTCTTCATCTT10540.1442562999217129No Hit
GTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10490.1435719721232228No Hit
CTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA10060.13768675305620795No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9880.1352231729816436No Hit
AAGTAGAGATGCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9850.13481257630254953No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9780.1338545173846634No Hit
TCTCTACTTTGGTCCCCTGGCCAAAGGCGGAATAAGTATCATACTGTTGG9740.13330705514587132No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9540.13056974395191093No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9470.1296116850340248No Hit
GAATAAGTATCATACTGTTGGCAGTAATAAGATGCAAAATCATCAGGCTG9410.12879049167583667No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCGCGATGGGGCTTCA9330.12769556719825248No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9300.12728497051915844No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9240.12646377716097032No Hit
GTAATAAGATGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG9200.12591631492217825No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9050.12386333152670795No Hit
GTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCC8970.12276840704912378No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8970.12276840704912378No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8950.12249467592972774No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8910.12194721369093568No Hit
GATCTACAAGGCGTCTATTTTAAACAGTGAAGCCCCATCGCGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA8870.1213997514521436No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8590.11756751578059904No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCACC8550.11702005354180696No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8550.11702005354180696No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8500.11633572574331685No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8490.11619886018361884No Hit
GTCCCAGATCCACTGCCGCTGAACCGCGATGGGGCTTCACTGTTTAAAAT8480.11606199462392082No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTT8470.1159251290642228No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8390.11483020458663863No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCATCTTATTACTGCCAACAGTATGATACTTATTCCGCCTTTGG8360.11441960790754457No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAGTAGTTGGCTGGCCTGGTATCAGCAGAAA8330.11400901122845052No Hit
CTACTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAGACAGGGTGGAAGGAGA8240.11277722119116834No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8230.11264035563147033No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8230.11264035563147033No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8220.11250349007177231No Hit
CAGTATGATACTTATTCCGCCTTTGGCCAGGGGACCAAAGTAGAGATGCAACGAACTGTGGCTGCACCAT8190.11209289339267825No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8180.11195602783298023No Hit
GCCCCTAACCTCCTGATCTACAAGGCGTCTATTTTAAACAGTGAAGCCCCATCGCGGTTCAGCGGCAGTGGAT8120.11113483447479211No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT8070.110450506676302No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8050.11017677555690596No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8030.10990304443750992No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTACAAGGCGTCTATTTTAAACAGTGAAGCCCCAT7990.10935558219871784No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACAATTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG7940.10867125440022773No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7800.10675513656445547No Hit
GTGCAGCCACAGTTCGTTGCATCTCTACTTTGGTCCCCTGGCCAAAGGCG7770.10634453988536141No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7730.10579707764656933No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7660.10483901872868319No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7660.10483901872868319No Hit
GGCCAAAGGCGGAATAAGTATCATACTGTTGGCAGTAATAAGATGCAAAA7610.10415469093019308No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7600.10401782537049506No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT7500.10264916977351488No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAGCCAACTACTA7320.10018558969895051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.033.06571
TACGTAT1751.7157254E-731.18715170
CAGGTAT752.3712946E-630.7807461
AGGGTAT554.257394E-429.9607092
ATCCCCG450.005792060429.28288312
GTGCTAG3300.028.9818691
CGGCGTA655.191829E-428.96165861
TATCAAC44200.028.2593212
CGCAGAG44550.028.025798
AACGCAG45250.027.7378446
TAGGACC607.1005296E-427.4527024
TCAACGC46500.026.8505154
ATCAACG46600.026.794733
GCAGAGT45750.026.6407999
CACGAAT500.00930738726.57107727
CAACGCA47050.026.5384585
TGTCAGG500.00960232826.40159422
GATTCAC2250.026.3834951
AGAGTAC46000.026.35459311
ACGCAGA47500.026.285247
Produced by FastQC (version 0.11.5)