FastQCFastQC Report
Wed 8 May 2024
EGAF00004692018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169409
Total Bases86.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50570.43244066019673183No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT43540.37232482390677685No Hit
GTATAATAACTGGCCTCCCTCCATCTACACTATTGGCCTGGGGACCAAGC39040.3338438476187544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38380.3281999710965111No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37960.3246084133096291No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37590.3214444219259472No Hit
CTATTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA35400.3027170134657763No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34900.2984413494337738No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31250.26722900200015565No Hit
CCTCTATGGTGCATCCACCAGGGCCCCTGGTATCCCAGCCAGGTTCAGTG29490.2521786646075069No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG29130.24910018650446505No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC28400.24285771701774145No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC27520.23533254832141703No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT26000.22233452966412948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25470.21780232579020684No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25020.21395422816140464No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT24890.21284255551308395No Hit
GTTATTATACTGCAGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC23420.20027210325899666No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22900.19582541266571404No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC22710.1942006603335531No Hit
GTTTATTACTGTCTGCAGTATAATAACTGGCCTCCCTCCATCTACACTAT22700.19411514705291305No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22510.1924903947207521No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA21860.18693203147914889No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21630.18496522602442772No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA20730.17726903076682324No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA20250.17316439329610084No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20130.17213823392842026No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19740.16880321598345832No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19210.16427101210953568No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18790.16067945432265357No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC18720.16008086135817323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG17620.15067440048776776No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17020.14554360364936475No Hit
GGCCAATAGTGTAGATGGAGGGAGGCCAGTTATTATACTGCAGACAGTAA16920.14468847084296427No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA16840.14400436459784388No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16590.14186653258184262No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16290.13930113416264114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16280.1392156208820011No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT16280.1392156208820011No Hit
CCATAGAGGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC16260.13904459432072097No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16040.13716330214663988No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT15790.13502547013063865No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15740.13459790372743838No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15680.1340848240435981No Hit
CTCCTGCAGGGCCAGTCAGACTATTGGCAGCAGCTTAGCCTGGTACCAGC15450.13211801858887692No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGGGGCCCTGGT15330.13109185922119634No Hit
CATCTACACTATTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGG15310.13092083265991625No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA14920.1275858147149543No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14750.12613208894407346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14360.12279707099911152No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14270.12202745147335108No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC14000.11971859289606973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.11817935384454883No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13820.11817935384454883No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13710.11723870775750829No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13590.11621254838982768No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13490.11535741558342719No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13460.11510087574150704No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13450.11501536246086698No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA13390.11450228277702669No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG13130.1122789374803854No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAGAGAGCCACCCTCTCCT13060.11168034451590504No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12960.11082521170950453No Hit
GTGTAGATGGAGGGAGGCCAGTTATTATACTGCAGACAGTAATAAACTGC12940.11065418514822445No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12880.11014110546438413No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT12620.10791776016774285No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12530.1071481406419824No Hit
GTCTGTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12330.10543787502918141No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11970.10235939692613961No Hit
TTATTACTGTCTGCAGTATAATAACTGGCCTCCCTCCATCTACACTATTG11870.10150426411973913No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAATAGTG11850.10133323755845902No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT11760.10056361803269856No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11740.10039259147141848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)