FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315167
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACACAGCCGTGTCCCCGGCTGTCAGGCTGTTCATTTGAAGATACA47950.364592481411106No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47920.3643643734978143No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41180.31311612897829705No Hit
GTATGGTAGTTCACCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT37440.28467867578794176No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36980.28118102111747023No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29070.2210365679795798No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28230.2146495464074144No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27600.2098592802282904No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26920.20468883419368034No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26010.19776956082383454No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24970.18986181982972505No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24640.18735263278351724No Hit
CTACCATACTGCTGACAGAAGTACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC24290.184691373795115No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23660.17990110761599096No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23650.17982507164489375No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23530.1789126399917273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23420.17807624430965802No Hit
GTGTATTCCTGTGCAAGAGAGCCAGGAGTACCTGGAAACTGGTATTTCGA23220.17655552488771387No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22990.17480669755247813No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22850.17374219395711724No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22670.17237354647736752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21900.1665187767028826No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTCCTTAGCCTGGTACC21510.16355337383009155No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21060.16013175513071723No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCCACGACATGCACTGGGTCCGCCAAGTTACAGGAAAAGGT20430.15534148895159322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20270.15412491341403792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20190.15351662564526025No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20090.15275626593428818No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19810.15062725874356642No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19160.14568492062224797No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18580.14127483429860999No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18090.13754907171484687No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17980.13671267603277756No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17840.1356481724374167No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17820.1354961004952223No Hit
GAGTATTAGCAGCAGCTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGG17800.13534402855302788No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16740.12728421561672396No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC16560.12591556813697424No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTACTTCTGTCAGCAGTATGGTAGTTCA16370.12447088468612731No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16070.12218980555321111No Hit
GTGTACTTCTGTCAGCAGTATGGTAGTTCACCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16060.12211376958211392No Hit
GTACTTCTGTCAGCAGTATGGTAGTTCACCTCGGACTTTTGGCCAGGGGA16040.1219616976399195No Hit
GCATAGTATGTGTCACCAGTAGTTGCAATAGCTGAGACCCACTCCAGACC15720.11952854656480888No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15680.11922440268042005No Hit
ATATTAGATGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGG15530.11808386311396195No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15350.11671521563421222No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGGAGCTGCTGCTAATACTCTGA15310.11641107174982342No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGATAATGCCAAGAACTCCGTGTATCTTCAAATGAA15290.116258999807629No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15220.11572674800994853No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15150.1151944962122681No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14910.11336963290593514No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14830.11276134513715748No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14800.11253323722386586No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG14470.11002405017765805No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14330.10895954658229716No Hit
CCCTGAGGAGACAGTGACCAGGGTGCCACGGCCCCAGAGATCGAAATACC14290.10865540269790833No Hit
GGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC14190.10789504298693625No Hit
GTATTAGCAGCAGCTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT14110.1072867552181586No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14050.10683053939157536No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14000.10645035953608932No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13890.10561396385402005No Hit
GTCTCCGGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG13600.10340892069220108No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13590.10333288472110386No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC13540.10295270486561782No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13530.10287666889452061No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13410.10196423724135414No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13310.10120387753038207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)