FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315167
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47100.3581294238678434No Hit
GGAATACACAGCCGTGTCCCCGGCTGTCAGGCTGTTCATTTGAAGATACA41490.3154732440823105No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37960.28863254628499646No Hit
GTATGGTAGTTCACCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT35210.2677226542332647No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33800.2570015823085586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30540.23221385573086914No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29630.22529458236102334No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28340.2154859420894837No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27440.2086427046907351No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23830.1811937191246435No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23580.17929281984721332No Hit
CTACCATACTGCTGACAGAAGTACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC23350.17754399251197756No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22780.1732099421594368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22310.1696362515178681No Hit
GTGTATTCCTGTGCAAGAGAGCCAGGAGTACCTGGAAACTGGTATTTCGA22090.1679634601537295No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21250.16157643858156415No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21130.1606640069283977No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTCCTTAGCCTGGTACC21090.16035986304400884No Hit
GAGTATTAGCAGCAGCTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGG20860.1586110357087731No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20570.15640599254695411No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20510.1559497767203709No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20380.15496130909610717No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCCACGACATGCACTGGGTCCGCCAAGTTACAGGAAAAGGT20110.15290833787648261No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19980.15191987025221892No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19950.15169176233892728No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19840.15085536665685803No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19330.14697753213090048No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18740.1424914098361653No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18700.14218726595177647No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18630.14165501415409604No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18400.13990618681886027No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18330.1393739350211798No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18270.13891771919459658No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18010.1369407839460692No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17880.13595231632180552No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17820.1354961004952223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16630.1264478199346547No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC16570.12599160410807145No Hit
GTGTACTTCTGTCAGCAGTATGGTAGTTCACCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16290.12386259691734966No Hit
GTACTTCTGTCAGCAGTATGGTAGTTCACCTCGGACTTTTGGCCAGGGGA16070.12218980555321111No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTACTTCTGTCAGCAGTATGGTAGTTCA15660.11907233073822565No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15630.11884422282493402No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15500.11785575520067033No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15240.11587881995214296No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15170.11534656815446252No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15050.11443413650129602No Hit
ATATTAGATGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGG14980.11390188470361559No Hit
GCATAGTATGTGTCACCAGTAGTTGCAATAGCTGAGACCCACTCCAGACC14960.11374981276142118No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14360.10918765449558877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14300.10873143866900552No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14250.10835125881351951No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGATAATGCCAAGAACTCCGTGTATCTTCAAATGAA13950.10607017968060331No Hit
GGGTGCCACGGCCCCAGAGATCGAAATACCAGTTTCCAGGTACTCCTGGC13670.10394117248988151No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGGAGCTGCTGCTAATACTCTGA13660.1038651365187843No Hit
CCCTGAGGAGACAGTGACCAGGGTGCCACGGCCCCAGAGATCGAAATACC13600.10340892069220108No Hit
GTATTAGCAGCAGCTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT13330.10135594947257649No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13220.10051955379050723No Hit
GTCTCCGGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG13210.10044351781941001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)