FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882533
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAC29290.33188560654389127No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28510.3230474101251738No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCACGGTTCTGGTTGTATACGAGCTGCAGTAATAATTAG27000.30593756834022073No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25310.2867881427663328No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24890.2820291139254849No Hit
GTTCTGGTTGTATACGAGCTGCAGTAATAATTAGCCTCGTCCTCAGCCTG24360.2760236727691769No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT24210.2743240196117312No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG23440.2655991334035101No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23130.2620865168781224No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22030.24962239372352085No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22010.24939577330252805No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21110.23919785435785407No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTCATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC21090.23897123393686126No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19560.22163477173091545No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18440.2089440281553211No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCGTAT18160.20577134226142255No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTCATAACTATGTCTCCTGGTACCAACAGCA18140.20554472184042974No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18120.205318101419437No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18110.2052047912089406No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18000.20395837889348048No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17580.19919935005263256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16920.19172087615987166No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16550.18752839837150567No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16360.18537550437207448No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16350.18526219416157808No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16310.18480895331959257No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16250.18412909205661432No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15680.17767041005832077No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15440.1749509650064077No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG15130.17143834848101996No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15120.1713250382705236No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14750.1671325604821576No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14070.15942746616840392No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13670.15489505774854878No Hit
AATTATTACTGCAGCTCGTATACAACCAGAACCGTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG13630.15444181690656328No Hit
CAACCAGAACCGTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA13390.15172237185465018No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCACGGTTCTGGTTGTATACGAGCTGCAGTAATAATTAGC13300.15070257996018277No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13230.14990940848670814No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13120.14866299617124798No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13120.14866299617124798No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13010.14741658385578782No Hit
GTAATAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12940.14662341238231316No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12860.14571693069834216No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATCTTTGATGTCATTAATCGGCCCTCAGGGGTTTCTAGTCG12800.1450370694353639No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12680.14367734690940737No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12670.143564036698911No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12500.14163776312047255No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12320.13959817933153776No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12200.1382384568055812No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11880.13461253006969712No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11710.13268625649125868No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATCTTTGATGTC11710.13268625649125868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.13223301564927317No Hit
TAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11400.12917363996587097No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACT11160.1264541949139579No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11130.12611426428246877No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11110.12588764386147602No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10980.12441461112502308No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10900.12350812944105205No Hit
CCCCTGAGGGCCGATTAATGACATCAAAGATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA10850.12294157838857017No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10690.12112861502062812No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10580.11988220270516797No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10560.11965558228417522No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10510.11908903123169332No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATCTTTGATGTCA10470.11863579038970781No Hit
GTCATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA10420.11806923933722592No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC10360.11738937807424764No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCGTATACAACCAGAACCGTGCTTTT10350.11727606786375126No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10290.11659620660077301No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10210.11568972491680198No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10190.11546310449580922No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10170.11523648407481646No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGA10160.11512317386432008No Hit
CTCATGATCTTTGATGTCATTAATCGGCCCTCAGGGGTTTCTAGTCGCTT10020.1135368309173708No Hit
GGTTGTATACGAGCTGCAGTAATAATTAGCCTCGTCCTCAGCCTGGAGCC9990.11319690028588165No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9980.11308359007538528No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9970.1129702798648889No Hit
GCTTGGTCCCTCCGCCGAAAAGCACGGTTCTGGTTGTATACGAGCTGCAGTAATAATTAGCCTCGTCCTCAGC9570.10843787144503378No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG9530.10798463060304826No Hit
GGCAAAGCCCCCAAACTCATGATCTTTGATGTCATTAATCGGCCCTCAGG9510.10775801018205551No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9450.10707814891907726No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA9180.10401877323567504No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9100.10311229155170402No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9070.10277236092021487No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9050.10254574049922212No Hit
GTATACAACCAGAACCGTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCC8990.10186587923624386No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT8880.10061946692078369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTA150.002726186566.50175512
CGTATGC950.051.16682441
TATCGGA200.00850755349.884817
TCGTATG1050.046.10830340
CTAGGCA1250.040.1124128
GACTAGG1350.037.0398626
TCTCCGA1350.036.9454211
GCCGTCT1550.036.3805946
TATACAC1750.036.1033
TCTCGTA1051.8189894E-1135.9231438
GGTATCA17350.035.4688951
AGGGGGG2351.8189894E-1235.369170
TGCCGTC1600.035.1129645
GTATCAA41700.035.0987781
TAGGCAT1450.034.63687529
CCGTCTT1650.034.30549247
ACGAGAC1500.033.26218422
GTATGCC1700.032.7885442
ATACACA1950.032.4001244
ACTAGGC1550.032.29353727
Produced by FastQC (version 0.11.5)