FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882533
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28070.31806176086333315No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAC27430.31080990739156494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24460.2771567748741407No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24330.27568374213768776No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCACGGTTCTGGTTGTATACGAGCTGCAGTAATAATTAG24240.2746639502432204No Hit
GTTCTGGTTGTATACGAGCTGCAGTAATAATTAGCCTCGTCCTCAGCCTG24140.27353084813825657No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG23700.268545198876416No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22800.25834727993174195No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22390.25370156130139043No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22230.2518885979334484No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTCATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC20130.22809345372920897No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19730.22356104530935386No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19240.21800884499503131No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19170.21721567352155668No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18910.2142696080486509No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18710.21200340383872332No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCGTAT18260.2069044443663863No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18220.2064512035244008No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18030.2042983095249696No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17890.20271196657802032No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17140.19421370079079195No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16730.18956798216044046No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTCATAACTATGTCTCCTGGTACCAACAGCA16710.18934136173944768No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16550.18752839837150567No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15970.18095640616271572No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15750.17846358153179542No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15690.17778372026881714No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15630.1771038590058389No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15380.1742711037434294No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14800.1676991115346395No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14600.16543290732471191No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14430.16350663374627353No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14360.16271346227279887No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14000.15863429469492926No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13740.1556882292220234No Hit
AATTATTACTGCAGCTCGTATACAACCAGAACCGTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG13740.1556882292220234No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13710.15534829859053428No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13590.15398857606457778No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13550.15353533522259225No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12500.14163776312047255No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12400.14050466101550876No Hit
CAACCAGAACCGTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA12390.1403913508050124No Hit
GTAATAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12270.13903162827905585No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12040.13642549343763916No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11880.13461253006969712No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11770.13336611775423696No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11560.130986603333813No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11290.1279272276504108No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCACGGTTCTGGTTGTATACGAGCTGCAGTAATAATTAGC11200.1269074357559434No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11060.12532109280899412No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11050.12520778259849774No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11050.12520778259849774No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11020.12486785196700861No Hit
GGTTGTATACGAGCTGCAGTAATAATTAGCCTCGTCCTCAGCCTGGAGCC10970.12430130091452671No Hit
CCCCTGAGGGCCGATTAATGACATCAAAGATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA10920.12373474986204483No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATCTTTGATGTCATTAATCGGCCCTCAGGGGTTTCTAGTCG10920.12373474986204483No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10890.12339481923055567No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10790.1222617171255919No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATCTTTGATGTC10710.12135523544162087No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10600.12010882312616072No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10420.11806923933722592No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10370.11750268828474404No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGACT10310.11682282702176576No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10110.1145566228118382No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT10090.11433000239084545No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10060.1139900717593563No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATCTTTGATGTCA9950.11274365944389615No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTAATTATTACTGCAGCTCGTATACAACCAGAACCGTGCTTTT9930.1125170390229034No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9860.11172386754942874No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC9800.11104400628645048No Hit
GTCATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA9770.11070407565496135No Hit
GTATACAACCAGAACCGTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCC9660.10945766333950119No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT9580.10855118165553015No Hit
GCTTGGTCCCTCCGCCGAAAAGCACGGTTCTGGTTGTATACGAGCTGCAGTAATAATTAGCCTCGTCCTCAGC9560.1083245612345374No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGA9550.10821125102404103No Hit
CTCATGATCTTTGATGTCATTAATCGGCCCTCAGGGGTTTCTAGTCGCTT9400.10651159786659535No Hit
GGCTGAGGACGAGGCTAATTATTACTGCAGCTCGTATACAACCAGAACCGTGCTTTTCGGCGGAGGGACCAAG9200.1042453936566678No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9130.10345222218319315No Hit
GTGCTGTTGGTACCAGGAGACATAGTTATGACTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA9070.10277236092021487No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC8950.10141263839425835No Hit
TAATTAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG8940.10129932818376197No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8860.10039284649979094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)