FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374788
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT85200.6197319150298083No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC81660.5939824903912457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58140.4229015673689325No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56790.4130818715321926No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54450.3960610654151767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA52320.38056776753943156No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG49570.36056468342755393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47230.3435438773105381No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46450.3378702752715328No Hit
GTGTAGGGGCTCTCCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCA46080.3351789512273893No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44960.3270322406072791No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44790.3257956863167267No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44690.3250683014399311No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40700.29604564485578866No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCC40030.2911721661812585No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39350.28622594901904874No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38580.280625085467723No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38380.2791703157141319No Hit
CACCAGGGAGAGCCCCTACACTCCTGATTTATTCTAGATTGAGTTTGCAC37600.2734967136751266No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37280.27116908206938084No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36690.2668775112962871No Hit
GTAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTTCCTAAGCCACTGCCA36150.2629496329615912No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCCCACGAGGGA35840.260694739843525No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32890.23923688597805626No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32890.23923688597805626No Hit
GACTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32050.23312685301297362No Hit
CTGTAAGTCTCTTGACAGTAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTT31570.229635405604355No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGATTCCACATGTGACAT31270.22745325097396832No Hit
GTCTCCATCCTCCCTCTCTGCATCCGTGGGAGACAGGGTCACCATCAGTTGCCGGGCAAGTCAGAATGTTGGCAC29960.21792450908794664No Hit
ACTTACTACTGTCAAGAGACTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA29270.21290555343805737No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29030.21115982973374803No Hit
CTCCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCAACATTCTGACTTGCCCGGCAACTGATGGTGACC28210.20519527374402452No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28050.20403145794115168No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27790.2021402572614832No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27620.2009037029709308No Hit
GTGCAAACTCAATCTAGAATAAATCAGGAGTGTAGGGGCTCTCCCTGGTG26380.19188413049866598No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25910.18846542157772692No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC25690.18686517484877668No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25180.18315551197711938No Hit
GAGTTTGCACAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGCTTAGGAA24320.17690000203667766No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24320.17690000203667766No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24080.17515427833236832No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23950.17420867799253412No Hit
CCCCTACACTCCTGATTTATTCTAGATTGAGTTTGCACAGCGGGGTCCCA23430.17042627663319726No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23410.17028079965783816No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23290.1694079378056835No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC23210.16882602990424705No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22790.16577101342170575No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22780.1656982749340262No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22730.1653345824956284No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22380.162788735426844No Hit
GGACTGTAAGTCTCTTGACAGTAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT22380.162788735426844No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21960.1597337189443027No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21820.15871538011678893No Hit
CTCTGCATCCGTGGGAGACAGGGTCACCATCAGTTGCCGGGCAAGTCAGA21600.1571151333878387No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21550.15675144094944093No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21510.1564604869987227No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21200.15420559388065652No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21120.15362368597922005No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21050.15311451656546318No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20880.15187796227491077No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20420.14853199184165122No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20310.14773186847717612No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20180.14678626813734189No Hit
GAGTGAAATCTGTTCCTAAGCCACTGCCACTGAACCTTGATGGGACCCCG20130.14642257569894415No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGATTCCACATGTGACATCCAGGTGACCCAGTCTCCATCCTCCCT20090.14613162174822591No Hit
ATGTAAATTGGTATCGGCAGACACCAGGGAGAGCCCCTACACTCCTGATT20030.14569519082214857No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20000.14547697535910992No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19700.14329482072872327No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19630.14278565131496637No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19530.14205826643817085No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGATTCCA19470.1416218355120935No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT19090.1388577729802704No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC18420.13398429430574024No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17880.13005641597104428No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17830.1296927235326465No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17830.1296927235326465No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17720.1288926001681714No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17610.12809247680369626No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17510.12736509192690074No Hit
CCTTGGTCCCTTGGCCGAACGCCCACGAGGGACTGTAAGTCTCTTGACAGTAGTAAGTTGCAGAATCTTCAGG17280.12569210671027098No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17250.1254738912472323No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17150.12474650637043674No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17020.12380090603060254No Hit
CTGCTGATGGTGAGAGTGAAATCTGTTCCTAAGCCACTGCCACTGAACCT16880.12278256720308876No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16600.12074588954806122No Hit
ATTCTAGATTGAGTTTGCACAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGCTTAGGAACAGATTTCACTCTCA16600.12074588954806122No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16510.12009124315894523No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16340.1188546888683928No Hit
GTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT16340.1188546888683928No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16290.11849099642999503No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTACTGTCAAGAGACTTACAGTCC16200.11783635004087903No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCCCACGA16010.11645431877496748No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCG15860.11536324145977417No Hit
ATCTAGAATAAATCAGGAGTGTAGGGGCTCTCCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCAACATT15770.11470859507065817No Hit
GTTCAGTGGCAGTGGCTTAGGAACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTA15630.11369025624314441No Hit
GCTGATGGTGAGAGTGAAATCTGTTCCTAAGCCACTGCCACTGAACCTTG15610.11354477926778529No Hit
GTCAAGAGACTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAC15390.11194453253883507No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC15140.11012607034684621No Hit
AATCAGGAGTGTAGGGGCTCTCCCTGGTGTCTGCCGATACCAATTTACAT15120.10998059337148709No Hit
CCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCAACATTCTGACTTGCCCGGCAACTGATGGTGACC14950.10874403908093465No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14820.10779843874110044No Hit
GTCAGAATGTTGGCACCTATGTAAATTGGTATCGGCAGACACCAGGGAGA14710.10699831537662535No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14560.10590723806143201No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14450.10510711469695691No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14430.1049616377215978No Hit
ACCTTGATGGGACCCCGCTGTGCAAACTCAATCTAGAATAAATCAGGAGT14390.10467068377087958No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14110.10263400611585205No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTACTGTCAAGAGACTTACAGTCCCTCGTGGGCGTT14020.10197935972673605No Hit
CATTCTGACTTGCCCGGCAACTGATGGTGACCCTGTCTCCCACGGATGCAGAGAGGGAGGATGGAGACTGGGTCA14020.10197935972673605No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGC13960.10154292880065871No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13930.10132471333762005No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT13900.10110649787458138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAT3550.050.8017632
TCGTATG3350.049.37105640
CGTATGC3500.049.3321241
CCGTCTT3900.045.7275247
ATCTCGT2500.045.1879837
ACGAGAC4200.045.0629922
GTCGTAT1150.044.82898339
TATACAC4300.044.778183
TCTCCGA4150.044.7605711
GCCGTCT3850.044.3928646
ATGCCGT4000.044.2808644
TGAGCGA4250.043.97769531
GTGGTAT2400.043.6563571
CTTATAC4350.043.5106741
CGAGACA4450.043.29557823
GGTATCA10100.043.1683461
GATGTCG1200.042.7144936
CGTCTTC4150.042.23521448
GTATGCC4100.042.19909742
CGAGCCC4500.042.03125415
Produced by FastQC (version 0.11.5)