FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374788
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70830.5152067082342878No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65160.4739639857199801No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58940.42872064638329693No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57470.41802808869440233No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG52740.3836227840219728No Hit
CACCAGGGAGAGCCCCTACACTCCTGATTTATTCTAGATTGAGTTTGCAC49370.35910991367396283No Hit
GTGTAGGGGCTCTCCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCA47740.34725354018219534No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA47230.3435438773105381No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43530.3166306368691027No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43190.31415752828799787No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41810.30411961698821927No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41100.29895518436297086No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41050.29859149192457307No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40870.2972821991463411No Hit
ACTTACTACTGTCAAGAGACTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA40540.29488182905291577No Hit
GACTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40040.29124490466893804No Hit
GTAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTTCCTAAGCCACTGCCA38410.2793885311771706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38350.27895210025109324No Hit
GAGTTTGCACAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGCTTAGGAA36420.2649135721289391No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34860.2535663680509286No Hit
GTCTCCATCCTCCCTCTCTGCATCCGTGGGAGACAGGGTCACCATCAGTTGCCGGGCAAGTCAGAATGTTGGCAC34560.25138421342054196No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34490.250875044006785No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT34120.2481837199626415No Hit
CCCCTACACTCCTGATTTATTCTAGATTGAGTTTGCACAGCGGGGTCCCA34090.24796550449960286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33840.24614704230761394No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33710.24520144196777974No Hit
CTGTAAGTCTCTTGACAGTAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTT31510.22919897467827766No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGATTCCACATGTGACAT31290.22759872794932745No Hit
CTCCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCAACATTCTGACTTGCCCGGCAACTGATGGTGACC29260.21283281495037779No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28930.21043244485695248No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28360.20628635105921786No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27750.201849303310765No Hit
GTGCAAACTCAATCTAGAATAAATCAGGAGTGTAGGGGCTCTCCCTGGTG27710.2015583493600468No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26930.1958847473210415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26930.1958847473210415No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC26760.19464819303048905No Hit
ATGTAAATTGGTATCGGCAGACACCAGGGAGAGCCCCTACACTCCTGATT26450.19239329991242285No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26270.19108400713419088No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26190.19050209923275443No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTACTGTCAAGAGACTTACAGTCC25490.18541040509518558No Hit
CTCTGCATCCGTGGGAGACAGGGTCACCATCAGTTGCCGGGCAAGTCAGA25320.18417385080463314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24790.18031871095761673No Hit
ATTCTAGATTGAGTTTGCACAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGCTTAGGAACAGATTTCACTCTCA24760.18010049549457807No Hit
GTTCAGTGGCAGTGGCTTAGGAACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTA24410.17755464842579366No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23940.17413593950485456No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23890.1737722470664568No Hit
GGACTGTAAGTCTCTTGACAGTAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT23200.1687532914165675No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23140.16831686049049016No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23050.16766221410137416No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22830.16606196737242396No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22570.16417076669275554No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTACTGTCAAGAGACTTACAGTCCCTCGTGGGCGTT22320.16235230450076665No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22170.16126122718557334No Hit
GAGTGAAATCTGTTCCTAAGCCACTGCCACTGAACCTTGATGGGACCCCG22000.1600246728950209No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21310.15500571724513162No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21180.15406011690529742No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGATTCCACATGTGACATCCAGGTGACCCAGTCTCCATCCTCCCT21160.15391463992993829No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20720.15071414647203787No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20550.14947759218148543No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20330.14787734545253522No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20090.14613162174822591No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGATTCCA19990.14540423687143036No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19990.14540423687143036No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC19860.14445863653159613No Hit
GTCAAGAGACTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAC19720.14344029770408237No Hit
GTGGGAGACAGGGTCACCATCAGTTGCCGGGCAAGTCAGAATGTTGGCACCTATGTAAATTGGTATCGGCAGACA19710.14336755921640282No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19470.1416218355120935No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19360.1408217121476184No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC18810.13682109532524286No Hit
CTCCTGATTTATTCTAGATTGAGTTTGCACAGCGGGGTCCCATCAAGGTT18610.1353663255716518No Hit
GCGGGGTCCCATCAAGGTTCAGTGGCAGTGGCTTAGGAACAGATTTCACT18360.1335478633796629No Hit
CTGCTGATGGTGAGAGTGAAATCTGTTCCTAAGCCACTGCCACTGAACCT18360.1335478633796629No Hit
GCCCCTACACTCCTGATTTATTCTAGATTGAGTTTGCACAGCGGGGTCCC18300.13311143245358556No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18220.13252952455214914No Hit
GTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT17730.12896533865585094No Hit
GTCAGAATGTTGGCACCTATGTAAATTGGTATCGGCAGACACCAGGGAGA17670.1285289077297736No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17660.12845616924209405No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17590.12794699982833715No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17300.12583758368563008No Hit
GTCTGCAGCCTGAAGATTCTGCAACTTACTACTGTCAAGAGACTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGA17270.1256193682225914No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17250.1254738912472323No Hit
GGGTCACCATCAGTTGCCGGGCAAGTCAGAATGTTGGCACCTATGTAAATTGGTATCGGCAGACACCAGGGAGAG17210.12518293729651409No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17170.12489198334579588No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17050.12401912149364121No Hit
CATCAAGGTTCAGTGGCAGTGGCTTAGGAACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTG16930.12314625964148654No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16890.12285530569076833No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16880.12278256720308876No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16830.12241887476469099No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCG16510.12009124315894523No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16440.11958207374518834No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16370.11907290433143146No Hit
GCTGATGGTGAGAGTGAAATCTGTTCCTAAGCCACTGCCACTGAACCTTG16270.11834551945463592No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16110.11718170365176304No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16030.11659979575032658No Hit
CTGCTGCTCTGGCTCCGAGATTCCACATGTGACATCCAGGTGACCCAGTC15810.1149995490213764No Hit
CCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTACTGTCAAGAG15790.11485407204601729No Hit
GTCCCATCAAGGTTCAGTGGCAGTGGCTTAGGAACAGATTTCACTCTCAC15580.11332656380474662No Hit
ATCTAGAATAAATCAGGAGTGTAGGGGCTCTCCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCAACATT15450.11238096346491241No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15360.1117263170757964No Hit
GCCTGAAGATTCTGCAACTTACTACTGTCAAGAGACTTACAGTCCCTCGT15330.11150810161275776No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG15320.1114353631250782No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15280.11114440917435997No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15280.11114440917435997No Hit
CCCTGGTGTCTGCCGATACCAATTTACATAGGTGCCAACATTCTGACTTGCCCGGCAACTGATGGTGACC14610.10627093049982979No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14510.10554354562303424No Hit
CATTCTGACTTGCCCGGCAACTGATGGTGACCCTGTCTCCCACGGATGCAGAGAGGGAGGATGGAGACTGGGTCA14450.10510711469695691No Hit
CTTACAGTCCCTCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAA14320.1041615143571227No Hit
ACCTTGATGGGACCCCGCTGTGCAAACTCAATCTAGAATAAATCAGGAGT14160.10299769855424983No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13980.10168840577601784No Hit
AATCAGGAGTGTAGGGGCTCTCCCTGGTGTCTGCCGATACCAATTTACAT13970.10161566728833828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT702.8244714E-547.50389570
GGTATCA10900.042.127341
GTATCAA29700.039.7890171
GTGGTAT3700.038.32651
CAGTTCG12500.035.64692370
TATCAAC33400.035.1598632
TCAACGC34600.033.9379734
ATCAACG34650.033.8902363
CAACGCA34800.033.742935
AACGCAG35400.033.2627036
AGAGTAC36250.032.0150511
CGCAGAG36650.031.8520588
ACGCAGA36900.031.8191247
CAGAGTA37150.031.23717510
GCAGAGT37800.030.8796429
AATGGTA7800.030.69482470
TGTTAGA22550.030.37721370
GTCTAGG450.00513730930.0122851
GTACGTA600.00550536429.5898269
ACGGGAT803.267709E-629.54334416
Produced by FastQC (version 0.11.5)