FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144413
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC117670.5487282533728344No Hit
GTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCG87270.4069645166299589No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG86290.4023945014323267No Hit
GTACCAAGGTTGTCTGATGATTGACAGTAATAATCAGCCTCGTCTTCTGC84190.3926016117231149No Hit
GGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCAC77230.36014517725829864No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTAGAGATAGATTGCCAATG76050.35464250589788443No Hit
GATTATTACTGTCAATCATCAGACAACCTTGGTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGT75820.353569951310685No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG74630.34802064714213166No Hit
GTATTATGTTTATTGGTATCAACAGAGGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCC74130.34568900673517644No Hit
CCCAAGTACCAAGGTTGTCTGATGATTGACAGTAATAATCAGCCTCGTCT73890.344569819339838No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT72790.3394402104445366No Hit
GTAATAATCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAATGTGG71710.33440386716551335No Hit
ACCTTGGTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA68900.3213000480784252No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGG68520.3195280013691393No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAAGTACCAAGGTTGTCTGATGATTGA67740.31589064233428915No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC66550.3103413381657358No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC66510.31015480693317937No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA64640.301434471811167No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAAGTACCAAGGTTGTCTGATGATTGACAGTAATAAT63420.29574526921819627No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC63050.2940198553170495No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT62450.29122188682870326No Hit
GGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCC61880.28856381676477433No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC61120.28501972334620246No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG59290.27648591945674644No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGGTTTT57670.2689314045382116No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG57530.2682785452242642No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG51690.24104498527102755No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC50730.23656823568967358No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA50680.23633507164897805No Hit
CTTCATGCGTGACCTGGCAGCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG50480.23540241548619598No Hit
ATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTAGAGATAGATTGC49480.2307391346722856No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC48220.22486340084675854No Hit
ACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGCCGTCTG47750.22267165886422066No Hit
GGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGG46620.2174021515445019No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA46230.2155834720270769No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG46200.21544357360265956No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG45910.21409122216662557No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGG45680.21301866757942617No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACA45270.21110672244572293No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT44360.20686313690506447No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC43050.20075423903884188No Hit
GCTCTAGAGATAGATTGCCAATGTATTATGTTTATTGGTATCAACAGAGG41980.19576452856795776No Hit
CACCAACACAGGGGCCTGGCCTGGCCTCTGTTGATACCAATAAACATAAT41640.19417901309122823No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA41200.19212716953310766No Hit
GTATCAACAGAGGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACA41180.19203390391682945No Hit
GGCCTGGCCTGGCCTCTGTTGATACCAATAAACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGC41060.19147431021916023No Hit
GTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCC41010.1912411461784647No Hit
CCCATGATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACG38750.18070213153902723No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC38670.1803290690739144No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA38490.17948967852741055No Hit
CTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGG38230.17827722551579384No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC37970.17706477250417713No Hit
CCTTGGTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA37870.1765984444227861No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACAC37190.17342741346932702No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT35340.16480034396359283No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCATCA34760.16209564109152483No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC34380.16032359438223887No Hit
GCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC34300.15995053191712605No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT33490.1561732744578586No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33120.15444786055671178No Hit
GTCCTGGGGACACTGCCACCGAGGGTGTCTGTGTCAGATCATGGGAGGCC32900.1534219387776515No Hit
CCACTGATGGTCAATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCG32810.15300224350439956No Hit
GACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCA32660.15230275138231303No Hit
GGCCTGGCCTCTGTTGATACCAATAAACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGCCGT32560.15183642330092198No Hit
ACAGAGGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCGATT32410.15113693117883542No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA32010.14927161885327128No Hit
CTGTTGATACCAATAAACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGA32000.14922498604513218No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGGTTTTGT31870.1486187595393238No Hit
ATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCC31810.14833896269048918No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC31240.14568089262656028No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30920.14418864276610896No Hit
GTAATACACGCCCGTGTCCTCCGTTTTCAGGCTGTTCATTTGCAGAAACA30660.14297618975449225No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC30660.14297618975449225No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG30490.1421834320161275No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT30260.1411108774289281No Hit
GGATCACCTGCTCTAGAGATAGATTGCCAATGTATTATGTTTATTGGTATCAACAGAGGCCAGGCCAGGCCC29840.13915229948708574No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCATCAGAC29560.13784658085919085No Hit
CCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCT29470.1374268855859389No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC29130.13584137010920938No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG28950.1350019795627055No Hit
ACACAGGGGCCTGGCCTGGCCTCTGTTGATACCAATAAACATAATACATT28680.1337428937429497No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC28540.13309003442900225No Hit
CAGCCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTAT28350.13220401107435928No Hit
GCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCGATTCTCTGGCT28170.1313646205278554No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC27940.130292065940656No Hit
CCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTAG27010.12595521478371935No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC26980.12581531635930204No Hit
GGACTCCACTGATGGTCAATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCCGAGGGC26810.12502255862093728No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG26760.12478939458024177No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC26380.12301734787095583No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT26010.12129193396980897No Hit
CGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTAGAGAT25920.12087223869655704No Hit
ACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAAGTACCAAGGT25920.12087223869655704No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC25880.12068570746400065No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG25820.120405910615166No Hit
GCCCTCGGGGATCCCTGAGCGATTCTCTGGCTCCACCTCAGGCACAACAG25680.11975305130121858No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAACCTACAGCTGC25230.11765457493495889No Hit
GTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCATCAGACAACCT25160.11732814527798516No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG25010.11662865315589861No Hit
CTCCACTGATGGTCAATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCCGAGGGCCTCT24940.11630222349892488No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC24380.11369078624313507No Hit
GGGATCCCCGAGGGCCTCTCAATGTCTTTAAGCATCACCAACACAGGGGC24010.11196537234198824No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT24000.11191873953384913No Hit
GGACAGACGGCCAGGATCACCTGCTCTAGAGATAGATTGCCAATGTATTA23950.11168557549315361No Hit
CCTCAGGCACAACAGCCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCAT23910.11149904426059717No Hit
GGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCGATTCTCTGGCTC23420.10921403666178112No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG23150.10795495084202529No Hit
GCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGGCA22920.10688239625482591No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22910.10683576344668681No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC22820.10641606817343488No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG22610.1054367792025137No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC22610.1054367792025137No Hit
GTCTTTAAGCATCACCAACACAGGGGCCTGGCCTGGCCTCTGTTGATACC22180.10343156845253224No Hit
GTGGAAGTCCCACAAAACCTACAGCTGCCAGGTCACGCATGAAGGGAGCA22170.10338493564439313No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA22150.10329167002811493No Hit
CAACAGCCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCATCAGACAAC22020.10268544352230657No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC21980.10249891228975017No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT21920.10221911544091554No Hit
GCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCCGAGGGCCTCTCAA21720.10128645927813344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATGG601.5370824E-766.0135770
TAACGAC200.00593337554.64723264
CGCGTAT700.054.1089439
TCGTATG3700.051.2776140
AGGGGGG7450.050.5070370
GACCGAT6100.049.52765326
TATCTCG4300.049.4065636
ACCGATC6000.049.25262527
CGTATGC4500.048.37795641
ATCGCGT1000.048.03441637
AGACCGA6300.047.93744725
ATGCCGT4450.047.62419544
CGATCAG6350.046.61084729
CCGATCA6350.046.57380328
TCAGTAT6200.046.2080332
AGTATCG1350.045.57610734
ATCTCGT4700.045.26039537
GTGGTAT4200.045.2274441
ACGAGAC6700.045.0492322
CCCACGA6750.044.7144919
Produced by FastQC (version 0.11.5)