FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144413
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC114470.5338057547683213No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAAGTACCAAGGTTGTCTGATGATTGA113570.529608802035802No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAAGTACCAAGGTTGTCTGATGATTGACAGTAATAAT98600.45979948825156347No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG90470.4218870152344721No Hit
GTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCG85180.3972182597288862No Hit
GTACCAAGGTTGTCTGATGATTGACAGTAATAATCAGCCTCGTCTTCTGC77080.3594456851362121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74470.347274522211906No Hit
GGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCAC73600.343217467903804No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG69990.3263830241655875No Hit
GATTATTACTGTCAATCATCAGACAACCTTGGTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGT68150.3178025874679924No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT67700.31570411110173274No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC67530.31491135336336795No Hit
GTATTATGTTTATTGGTATCAACAGAGGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCC66170.30856929145644985No Hit
GTAATAATCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAATGTGG64970.3029733544797574No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC64790.30213396393325354No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTAGAGATAGATTGCCAATG64620.30134120619488874No Hit
ACCTTGGTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA64240.2995691594856028No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG63400.29565200360191807No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGG62310.2905690275147558No Hit
CCCAAGTACCAAGGTTGTCTGATGATTGACAGTAATAATCAGCCTCGTCT61110.2849730905380633No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC60360.28147562992763053No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT59960.2796103176020664No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA58060.27075008405563666No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGGTTTT57360.2674857874858994No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG57100.2662733344742827No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC56710.26445465495685766No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACAC54720.255174726137176No Hit
GGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCC54510.2541954371662548No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG52910.2467341878639982No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC51730.24123151650358396No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG48610.22668208036418358No Hit
CTTCATGCGTGACCTGGCAGCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG47610.2220187995502732No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA46280.21581663606777238No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA45410.21175958175967038No Hit
ATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTAGAGATAGATTGC44920.20947457416085427No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG43760.20406516841671823No Hit
GGCCTGGCCTGGCCTCTGTTGATACCAATAAACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGC43490.20280608259696242No Hit
ACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGCCGTCTG43060.20080087184698095No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGG42590.1986091298644431No Hit
GTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCC40800.1902618572075435No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT40730.1899354275505698No Hit
ACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCAAGTACCAAGGT40490.1888162401552313No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACA39820.18569184200991135No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC39580.18457265461457284No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC39370.18359336564365167No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA39370.18359336564365167No Hit
CACCAACACAGGGGCCTGGCCTGGCCTCTGTTGATACCAATAAACATAAT37690.1757590538762822No Hit
GCTCTAGAGATAGATTGCCAATGTATTATGTTTATTGGTATCAACAGAGG37430.17454660086466553No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC37250.17370721031816166No Hit
CCTTGGTACTTGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA37020.17263465573096226No Hit
GTATCAACAGAGGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACA36700.17114240587051097No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36070.1682045389577474No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA35130.16382105499267166No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC34990.1631681956787242No Hit
GCTGTAGGTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC34160.15929767260317856No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC34120.15911114137062218No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT33980.1584582820566747No Hit
GGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGG33710.15719919623691891No Hit
CTGCACAGGCTCTGAGGCCTCCCATGATCTGACACAGACACCCTCGGTGG33270.15514735267879834No Hit
GGCCTGGCCTCTGTTGATACCAATAAACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGCCGT33100.15435459494043358No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT33030.15402816528345986No Hit
CCCATGATCTGACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACG32590.1519763217253393No Hit
GTCCTGGGGACACTGCCACCGAGGGTGTCTGTGTCAGATCATGGGAGGCC32380.15099703275441811No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC32150.14992447816721874No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC31730.14796590022537637No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC31450.14666018159748145No Hit
GCTTGGTCCCGCCGCCGAACACCCAAGTACCAAGGTTGTCTGATGATTGACAGTAATAATCAGCCTCGTCTTCTG31380.1463337519405077No Hit
CTGTTGATACCAATAAACATAATACATTGGCAATCTATCTCTAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGA31240.14568089262656028No Hit
ATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCC31190.14544772858586474No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACC30430.14190363516729287No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA29350.13686729188826965No Hit
GACACAGACACCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCA29270.1364942294231568No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGGTTTTGT28940.13495534675456639No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC28830.13444238586503626No Hit
CCACTGATGGTCAATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCG28820.13439575305689716No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT28460.13271697196388943No Hit
GTAATACACGCCCGTGTCCTCCGTTTTCAGGCTGTTCATTTGCAGAAACA28340.13215737826622018No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCATCAGAC27790.12959257381856948No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCATCA27600.12870655046392648No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC27550.12847338642323097No Hit
GGATCACCTGCTCTAGAGATAGATTGCCAATGTATTATGTTTATTGGTATCAACAGAGGCCAGGCCAGGCCC27410.12782052710928352No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG27370.1276339958767271No Hit
GGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCGATTCTCTGGCTC27220.12693450375464055No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG27140.12656144128952773No Hit
ACAGAGGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCGATT26680.12441633211512894No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG26410.12315724629537314No Hit
CCCCTGTGTTGGTGATGCTTAAAGACATTGAGAGGCCCTCGGGGATCCCT26090.1216649964349218No Hit
GGACTCCACTGATGGTCAATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCCGAGGGC26030.1213851995860872No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAACCTACAGCTGC25750.12007948095819228No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG25400.11844733267332365No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG25250.11774784055123709No Hit
ACACAGGGGCCTGGCCTGGCCTCTGTTGATACCAATAAACATAATACATT25000.1165820203477595No Hit
CGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTAGAGAT24920.11620895788264667No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC24890.11606905945822936No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC24810.11569599699311653No Hit
GCTTAAAGACATTGAGAGGCCCTCGGGGATCCCTGAGCGATTCTCTGGCT24440.1139705830919697No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC24270.11317782535360493No Hit
GCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCCGAGGGCCTCTCAA24040.11210527076640554No Hit
CAGCCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTAT23610.11010006001642408No Hit
GGGATCCCCGAGGGCCTCTCAATGTCTTTAAGCATCACCAACACAGGGGC23560.10986689597572855No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC23510.10963373193503305No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG23280.10856117734783365No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC23250.10842127892341635No Hit
GTGGAAGTCCCACAAAACCTACAGCTGCCAGGTCACGCATGAAGGGAGCA23080.10762852118505158No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT23040.10744198995249515No Hit
GGTATCAACAGAGGCCAGGCCAGGCCCCTGTGTTGGTGATGCTTAAAGAC22970.10711556029552143No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG22920.10688239625482591No Hit
GTCCAGGCAGAAGACGAGGCTGATTATTACTGTCAATCATCAGACAACCT22880.10669586502226949No Hit
CCCTCGGTGGCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTAG22710.10590310728390473No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT22590.10534351358623549No Hit
CTCCACTGATGGTCAATGTGGCTGTTGTGCCTGAGGTGGAGCCAGAGAATCGCTCAGGGATCCCCGAGGGCCTCT22410.10450412303973163No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC22280.10389789653392327No Hit
GTCTTTAAGCATCACCAACACAGGGGCCTGGCCTGGCCTCTGTTGATACC22220.10361809968508864No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG21970.10245227948161104No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA21960.10240564667347195No Hit
GCCCTCGGGGATCCCTGAGCGATTCTCTGGCTCCACCTCAGGCACAACAG21620.10082013119674241No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC21460.10007400626651675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4650.040.0866931
ATTTCGC400.001979554536.46223464
TACGTAT800.002624045834.4251270
CGCGCGT1004.656613E-1033.86933514
GAGTGTA400.002845276633.86301410
GTGCGCG1253.6379788E-1232.50849511
CTTATTA1101.2405508E-930.90784629
TATAGGG553.627619E-430.7938982
AATGGTA4950.030.60011170
ATCAACG41700.029.8853033
TCAACGC42000.029.7524664
CAACGCA42750.029.3097085
AACGCAG43000.029.218746
ATAATAG1202.8048817E-928.63599438
TAACCGT755.2092677E-528.29149653
ACGCAGA45250.027.8407157
TATAAGA1104.3806722E-827.7145082
GGTTACT1154.537833E-827.61390552
TGTGCGC1353.8198777E-1027.59208510
CGCAGAG45850.027.5496068
Produced by FastQC (version 0.11.5)