FastQCFastQC Report
Wed 8 May 2024
EGAF00004692029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748073
Total Bases129.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCGCAAACAAGAAGAACTACTTAGCTTGGTTCCAGCAGAC192161.0992675935158314No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATCGTGGCCCTCTCGCCCA136330.7798873388010684No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC125390.7173041400444947No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT118480.6777748984167137No Hit
CATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCCACCCGGGAA92470.5289824852852255No Hit
CTTATATATTGGTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCT91940.5259505752906201No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC91740.5248064583115236No Hit
ATATATAAGTATTCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCA90930.5201727845461831No Hit
ACTTAGGAGGCTGTCCTGATGTCTGCTGGAACCAAGCTAAGTAGTTCTTC82900.47423648783546224No Hit
GTCTCTGGGCGAGAGGGCCACGATCAACTGCAAGTCCAGCCAGAGTGTTT78740.4504388546702569No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC77780.4449470931705941No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC75120.4297303373486118No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG71180.4071912328604126No Hit
GTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA67990.38894256704382485No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT67450.38585345120026454No Hit
GTCCTGATGTCTGCTGGAACCAAGCTAAGTAGTTCTTCTTGTTTGCGCTG66680.38144860083074333No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCCACCCGGGAATCTGGGGTCCCT66410.3799040429089632No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66400.3798468370600084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA66210.37875992592986674No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG62810.3593099372852278No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT62710.3587378787956796No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA62270.3562208214416675No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA61930.3542758225772036No Hit
GTCTCCAGACTCACTGGCTCTGTCTCTGGGCGAGAGGGCCACGATCAACT57590.32944848413081146No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG57380.32824716130276027No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT55480.31737805000134434No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC54930.3142317283088292No Hit
GTTTATTACTGTCACCAATATATAAGTATTCCATACACTTTTGGCCAGGG53360.3052504100229224No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC53300.30490717492919345No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC52770.30187526493458794No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA52530.3005023245596723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50920.29129218287794617No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG43940.2513625003074815No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG41330.23643177373027327No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC40910.2340291280741708No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT39710.22716442619959235No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG39380.22527663318408328No Hit
GTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCAGATTCCCGGGT38970.22293119337693565No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC38820.22207310564261334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA38450.21995648923128497No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC37790.2161809032002668No Hit
CAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGT35740.2044537041645286No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT35320.20205105850842614No Hit
TAAGTATTCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA34580.19781782568576942No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA34530.19753179644099533No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTA34430.1969597379514471No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCACTGGCTCTGTCTC33910.19398503380579643No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC33620.19232606418610665No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA33450.1913535647538747No Hit
GGCCAAAAGTGTATGGAATACTTATATATTGGTGACAGTAATAAACTGCC33430.19123915305596506No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT33330.19066709456641687No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA33220.19003783022791382No Hit
CACTGGCTCTGTCTCTGGGCGAGAGGGCCACGATCAACTGCAAGTCCAGC33060.1891225366446367No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC32510.18597621495212155No Hit
CCCAGAGACAGAGCCAGTGAGTCTGGAGACTGGGTCATCACGATGTCCCC32380.1852325389157089No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC32380.1852325389157089No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC31960.18282989325960644No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGT31890.18242945231692267No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG31880.18237224646796787No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA31850.18220062892110342No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT31370.17945474817127202No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC31260.178825483832769No Hit
GTCAGGGACCCCAGATTCCCGGGTGGATGCCCAGTAAATGAGCAACTTAG31230.17865386628590452No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT31090.17785298440053704No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA30770.1760223972339828No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC30190.1727044579946032No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA30070.17201798780714536No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC29890.17098828252595857No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG29630.16950093045313325No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC29570.16915769535940434No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA29470.16858563686985611No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT29420.168299607625082No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCACC29290.16755593158866935No Hit
GCCTGAAGATGTGGCAGTTTATTACTGTCACCAATATATAAGTATTCCAT28980.16578255027106992No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC28870.1651532859325669No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC28470.16286505197437406No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG28390.1624074051827355No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT28340.1621213759379614No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCA27850.1593182893391752No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT27390.15668682028725345No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA27150.15531387991233775No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA26760.15308285180309975No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCA26380.15090902954281657No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG26290.1503941769022232No Hit
CTCCAGACTCACTGGCTCTGTCTCTGGGCGAGAGGGCCACGATCAACTGC25970.14856358973566894No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG25870.14799153124612074No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGATGTCTGCTGGAACCAAGCTA24900.14244256389750312No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATG24700.1412984469184067No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT24520.14026874163721997No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC24340.13923903635603319No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT24270.13883859541334945No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC24250.13872418371543982No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT24150.1381521252258916No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC23780.13603550881456325No Hit
GTAATAGAGAGCCGTGTCTTCAGATCTCAGGCTGCTCATCTCCAGGTAGG23510.1344909508927831No Hit
GCCAATGAATCGGTCAGGGACCCCAGATTCCCGGGTGGATGCCCAGTAAA23500.13443374504382824No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23460.13420492164800898No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGT23420.1339760982521897No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23200.13271756957518366No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA23200.13271756957518366No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG22630.1294568361847589No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA22590.12922801278893958No Hit
CTCTATTACTGTGCGAGGCGGACTGATGCTTTTGATTTGTGGGGCCAAGG22570.12911360109102996No Hit
GAACTACTTAGCTTGGTTCCAGCAGACATCAGGACAGCCTCCTAAGTTGC22460.12848433675252693No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT22300.1275690431692498No Hit
ACTTAGCTTGGTTCCAGCAGACATCAGGACAGCCTCCTAAGTTGCTCATT22200.12699698467970158No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC21930.12545242675792143No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC21820.1248231624194184No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCCACCCGGGAATCTGGGGTCC21570.1233930161955479No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG21180.1211619880863099No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG21150.12099037053944545No Hit
ATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC21030.1203039003519876No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC20920.11967463601348456No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA20730.11858772488334296No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA20620.11795846054483995No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG20480.11715757865947247No Hit
CCCCAGATTCCCGGGTGGATGCCCAGTAAATGAGCAACTTAGGAGGCTGT20460.1170431669615628No Hit
CATTTACTGGGCATCCACCCGGGAATCTGGGGTCCCTGACCGATTCATTG20320.11624228507619533No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC20280.11601346168037606No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAGCGCAAACAAGAAGAACTACT20160.11532699149291821No Hit
TTATTACTGTCACCAATATATAAGTATTCCATACACTTTTGGCCAGGGGA20140.11521257979500855No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20060.11475493300336999No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19920.1139540511180025No Hit
GTGTATGGAATACTTATATATTGGTGACAGTAATAAACTGCCACATCTTC19820.1133819926284543No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT19790.11321037508158983No Hit
GGCCACGATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCGCAAACA19500.11155140546190005No Hit
CAAGAAGAACTACTTAGCTTGGTTCCAGCAGACATCAGGACAGCCTCCTA19490.11149419961294522No Hit
GTTCCAGCAGACATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCAT19440.11120817036817113No Hit
GAATACTTATATATTGGTGACAGTAATAAACTGCCACATCTTCAGGCTGC19430.1111509645192163No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19370.11080772942548738No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT19300.11040728848280364No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT19020.10880552471206867No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18370.10508714453000532No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG18350.10497273283209568No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG18300.10468670358732157No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTATGG18010.10302773396763179No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT17630.1008539117073486No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG17510.10016744151989075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)