FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748073
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCGCAAACAAGAAGAACTACTTAGCTTGGTTCCAGCAGAC164950.9436104785097648No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT113590.6498012382778064No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATCGTGGCCCTCTCGCCCAGAGACAGAGCCAGTGAGTCTGGAGA111360.6370443339608814No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC92770.5306986607538702No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC81710.4674289918098386No Hit
ATATATAAGTATTCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT81430.46582722803910365No Hit
CTTATATATTGGTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC79440.4544432640970943No Hit
CATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCCACCCGGGAA76670.43859724393660904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC76240.43613739243155175No Hit
ACTTAGGAGGCTGTCCTGATGTCTGCTGGAACCAAGCTAAGTAGTTCTTCTTGTTTGCGCTGTATAAAACACT69950.40015491343896964No Hit
GTCTCTGGGCGAGAGGGCCACGATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCGCAAACAAGAAGAACTA65600.3752703691436227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT65150.3726961059406558No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC62950.3601108191705953No Hit
GTCCTGATGTCTGCTGGAACCAAGCTAAGTAGTTCTTCTTGTTTGCGCTG62690.35862346709776993No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCCACCCGGGAATCTGGGGTCCCT60900.3483836201348571No Hit
GTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA60010.34329229957787805No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC57930.3313934829952754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT56680.3242427518759228No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55020.3147465809494226No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA54140.3097124662413984No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT53270.304735557382329No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA53180.30422070474173557No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG49990.2859720389251479No Hit
GTTTATTACTGTCACCAATATATAAGTATTCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA49950.2857432155293286No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG49570.2835693932690454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT49380.2824824821389038No Hit
GTCTCCAGACTCACTGGCTCTGTCTCTGGGCGAGAGGGCCACGATCAACTGCAAGTCCAGCCAGAGTGTTTTATA49250.28173880610249113No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC48650.2783064551652019No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG47500.27172778253539753No Hit
GTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCAGATTCCCGGGT46400.2654351391503673No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45570.2606870536871172No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT45460.2600577893486142No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG42690.24421176918812884No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG41500.23740427316250526No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG41490.23734706731355038No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA40560.23202692336075212No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG38350.21938443074173675No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCA36920.21120399434119744No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA35890.2053117918988509No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC35770.20462532171139305No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACC35170.2011929707741038No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG34880.19953400115441403No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA34650.19821826662845318No Hit
GGCCAAAAGTGTATGGAATACTTATATATTGGTGACAGTAATAAACTGCC33180.18980900683209453No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33130.18952297758732045No Hit
CAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGT32960.1885504781550885No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCACCAATATATAAGTATT32520.1860334208010764No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA30810.17625122062980206No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30430.1740773983695189No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29880.17093107667700375No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT29760.17024460648954592No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC29410.1682424017761272No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT29240.16726990234389524No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC29200.16704107894807596No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC29020.1660113736668892No Hit
TAAGTATTCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA28430.16263622857855478No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA28410.16252181688064513No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT28280.16177814084423248No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC27890.15954711273499447No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC27680.15834578990694326No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27370.15657240858934382No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27190.15554270330815703No Hit
GCCTGAAGATGTGGCAGTTTATTACTGTCACCAATATATAAGTATTCCATACACTTTTGGCCAGGGGACCAAG27180.1554854974592022No Hit
GTCAGGGACCCCAGATTCCCGGGTGGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGATGTCTGCTGGAA26850.15359770444369314No Hit
CACTGGCTCTGTCTCTGGGCGAGAGGGCCACGATCAACTGCAAGTCCAGC26640.1523963816156419No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26240.1501081476574491No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCACTGGCTCTGTCTCTGGGCGAGAGGGCCACGATCAACTG25820.14770550200134663No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25650.14673300256911467No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC25590.14638976747538576No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT25560.14621814992852128No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG25400.14530285634524417No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA25260.14450197445987667No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCACC25060.14335785748078028No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24990.14295741653809652No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24910.14249976974645795No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA24400.13958227144976212No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC24110.13792330183007231No Hit
CCCAGAGACAGAGCCAGTGAGTCTGGAGACTGGGTCATCACGATGTCCCC23990.13723683164261447No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23820.1362643322103825No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCACTGGCTCTGTCTCTGGGCGAGAGGG23810.1362071263614277No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGT23800.13614992051247288No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA23260.13306080466891257No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT23170.1325459520283192No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22870.13082977655967457No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATG22360.12791227826297874No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT22290.12751183732029497No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT22190.12693977883074678No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22150.12671095543492747No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA22070.12625330864328893No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22060.1261961027943341No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20970.11996066525825867No Hit
GTAATAGAGAGCCGTGTCTTCAGATCTCAGGCTGCTCATCTCCAGGTAGG20860.11933140091975564No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC20760.11875934243020744No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20700.11841610733647852No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20680.11830169563856888No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGATGTCTGCTGGAACCAAGCTA20550.11755801960215621No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20240.11578463828455676No Hit
GCCAATGAATCGGTCAGGGACCCCAGATTCCCGGGTGGATGCCCAGTAAA20200.11555581488873748No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19980.11429728621173144No Hit
GTGTATGGAATACTTATATATTGGTGACAGTAATAAACTGCCACATCTTC19880.11372522772218324No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19820.1133819926284543No Hit
CTCTATTACTGTGCGAGGCGGACTGATGCTTTTGATTTGTGGGGCCAAGG19810.11332478677949949No Hit
ACTTAGCTTGGTTCCAGCAGACATCAGGACAGCCTCCTAAGTTGCTCATT19660.11246669904517717No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG19660.11246669904517717No Hit
ATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC19310.11046449433175845No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG19090.10920596565475241No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG18980.10857670131624937No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT18920.10823346622252045No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG18840.10777581943088188No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA18780.10743258433715296No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18770.10737537848819814No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18630.10657449660283067No Hit
TTATTACTGTCACCAATATATAAGTATTCCATACACTTTTGGCCAGGGGA18560.10617405566014691No Hit
CTCCAGACTCACTGGCTCTGTCTCTGGGCGAGAGGGCCACGATCAACTGC18420.10537317377477944No Hit
GAACTACTTAGCTTGGTTCCAGCAGACATCAGGACAGCCTCCTAAGTTGC18280.10457229188941194No Hit
GAATACTTATATATTGGTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT18220.10422905679568302No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18200.10411464509777338No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCCACCCGGGAATCTGGGGTCCCTGACCGATTCATTGGCAGCGGGT18120.1036569983061348No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT18060.1033137632124059No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17670.1010827351031679No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17570.10051067661361968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG150.002283525469.5439146
TCAGCAT8450.045.26284870
GGTATCA13450.044.8105621
ACCGGAA5750.043.5799170
GTGGTAT3650.039.181931
GTATCAA31750.036.4640241
TGGTATC4100.035.7038082
TATGCGG400.00259427934.50654639
TATCAAC36100.031.7799052
TCTCGCC28000.031.67570742
CGCCCAG28450.031.63033545
TCGCCCA28400.031.53235244
CCTCTCG28500.031.04310240
CTCTCGC28550.030.90991241
GCCCAGA29400.030.63243746
TTATGCG450.00464928230.63158838
ATCAACG37800.030.523783
TCAACGC37900.030.4441174
TTAGTAC450.004940341730.253663
TCTATTA6900.030.0961282
Produced by FastQC (version 0.11.5)