FastQCFastQC Report
Wed 8 May 2024
EGAF00004692031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749145
Total Bases129.8 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC56950.3255876442490474No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA46300.26470075379685504No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC42790.2446338068027522No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42120.240803363929234No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT42050.24040316840513506No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCCAGTG41040.23462891870027927No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC40190.2297694016219353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.22296607771225369No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37840.2163342661700431No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36540.20890206357963462No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG34610.19786810127233592No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32960.18843492106143286No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31860.18214613425416418No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC31750.1815172555734373No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA31520.18020232742282657No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCAATTGGGTGTTCGGCGGAGG30880.17654339691677934No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA30160.1724271000974762No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA30040.17174105062759235No Hit
GCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT29050.16608114250105052No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA28740.1643088480371839No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28610.16356562777814304No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28040.1603068927961947No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC27740.15859176912148507No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT26860.1535607396756701No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT26680.15253166547084435No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26150.14950161364552395No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA25610.1464143910310466No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGA25200.1440703886756101No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA24730.14138336158523163No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24720.14132619079607467No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT24450.13978257948883596No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24410.13955389633220802No Hit
CTATTACTGTCAGACCTGGGGCACTGGCAATTGGGTGTTCGGCGGAGGGA23750.13578062424784682No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG23500.1343513545189221No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATG22390.1280053969224964No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGC21850.12491817430801905No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21730.1242321248381352No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21640.12371758773572232No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTC21340.12200246406101267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.12097338985618687No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC21080.12051602354293098No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT21040.12028734038630302No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG20970.1198871448622041No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA20910.11954412012726218No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAG20830.11908675381400627No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGAT20730.11851504592243639No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20610.11782899645255253No Hit
GGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC20450.11691426382604071No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCCAGTGC20270.11588518962121494No Hit
CCCAATTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA20190.11542782330795903No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCT20090.11485611541638915No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19860.11354118726577841No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19720.11274079621758058No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG19150.10948206123563226No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19070.10902469492237636No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18990.10856732860912047No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTG18820.10759542519345165No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18390.10513708125970117No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTA18270.10445103178981731No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18230.10422234863318935No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCAATT18130.10365064074161948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)