FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187970
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66350.5585157874357096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58950.4962246521376803No Hit
GTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGCC55930.47080313475929525No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49750.4187816190644545No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46800.3939493421551049No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAA45620.3840164313913651No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45500.3830063048730187No Hit
GCTTCATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT45080.3794708620588062No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT42620.35876326843270456No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41920.3528708637423504No Hit
ACTTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAA40830.3436955478673704No Hit
GTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCG39210.33005883986969364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38750.3261866882160324No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35940.30253289224475366No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35500.2988290950108168No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34840.2932733991599114No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTG34830.29318922195004926No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34420.289737956345699No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33210.2795525139523725No Hit
CAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCG32430.2729866915831208No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32300.27189238785491215No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31720.26701010968290445No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31610.26608416037442023No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31290.2633904896588298No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30940.26044428731365266No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30750.2588449203262709No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT30190.2541309965739875No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29910.2517740346978459No Hit
CTCCTAACCTCCTGATCTCTGATGCATCCACTTTACAAAGTGGGGTCCCA29840.2511847942288105No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29710.2500904905006019No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28310.2383056811198936No Hit
AGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAA28230.237632263440996No Hit
TCTCTGATGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACT27710.23325504852816148No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27190.22887783361532696No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27090.22803606151670497No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC25920.21818732796282736No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC25220.2122949232724732No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTGGATGCATCAGAGATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTG24300.20455061996515062No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24070.20261454413832No Hit
GGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAATTCCTA22810.19200821569568255No Hit
TACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCAT22770.19167150685623374No Hit
TTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGA22480.1892303677702299No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22440.18889365893078108No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGACGATTTTG22270.18746264636312363No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22210.18695758310395044No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22140.18636834263491503No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21700.18266454540097815No Hit
GACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCT21470.1807284695741475No Hit
GGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTCCTAACCTCCTGATCTCTGATGCATC21450.1805601151544231No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21390.18005505189524987No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCTGCAGCTTCATACTTAC21230.17870821653745464No Hit
GGGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGGCTG20820.17525695093310437No Hit
CATCAGAGATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAATTCCTAATGTCCTGA20240.17037467276109666No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19890.16742847041591957No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19870.16726011599619517No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19740.16616581226798657No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19710.16591328063839994No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19630.16523986295950235No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19340.1627987238734985No Hit
CTCACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCT19230.16187277456501425No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19110.16086264804666786No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19100.16077847083680563No Hit
GAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTCCTAACCTCC18940.1594316354790104No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18820.158421508960664No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18810.15833733175080178No Hit
CACCATCACTTGCCGGGCCAGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTC18550.15614872429438453No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18520.15589619266479793No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG18380.1547177117267271No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18360.1545493573070027No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC18000.15151897775196344No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17930.15092973728292802No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17920.15084556007306582No Hit
ATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGA17880.150508851233617No Hit
GGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATT17750.1494145475054084No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17490.14722594004899112No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17470.14705758562926674No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17310.14571075027147148No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG17070.14369049723477867No Hit
CACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTT16780.14124935814877482No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16730.1408284720994638No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGG16480.13872404185290874No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16110.13560948508800727No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15900.13384176368090103No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15830.13325252321186562No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA15690.1320740422737948No Hit
GCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGA15520.13064302970613736No Hit
GATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCGCTGAACCTTGATG15130.12736011852151147No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15050.12668670084261388No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC15030.12651834642288948No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA15020.12643416921302725No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14910.12550821990454303No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGG14850.12500315664536985No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGG14800.12458227059605884No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14610.12298290360867699No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14560.12256201755936598No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14450.12163606825088176No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAA14380.12104682778184635No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGC14210.1196158152141889No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14180.11936328358460231No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACT14120.11885822032542909No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14050.11826897985639367No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13990.11776391659722046No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGT13890.11692214449859845No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13850.11658543565914964No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13820.11633290402956303No Hit
TCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGC13820.11633290402956303No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13820.11633290402956303No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC13730.11557530914080322No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13680.11515442309149222No Hit
GGACCCCACTTTGTAAAGTGGATGCATCAGAGATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGG13670.11507024588163No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAA13640.1148177142520434No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG13630.11473353704218119No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA13540.11397594215342137No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13220.11128227143783091No Hit
CTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACAATCAGCAGC13190.11102973980824432No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT13140.11060885375893331No Hit
GTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTCCTAACCTCCTGATCTCTGATG13130.1105246765490711No Hit
GGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC13030.10968290445044909No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC12970.10917784119127587No Hit
GTTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACAATCAGCAGCC12850.10816771467292946No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12830.10799936025320506No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12800.10774682862361844No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12790.10766265141375624No Hit
GTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCAC12750.10732594257430743No Hit
TTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACAATCAGCAGCCTG12690.10682087931513423No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG12650.10648417047568542No Hit
GCTGATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCGCTGAACCTTG12460.10488480348830358No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12440.10471644906857916No Hit
GATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT12230.10294872766147294No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA12150.10227530998257531No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT11920.10033923415574467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)