FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187970
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67380.5671860400515164No Hit
GTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGCC56930.4792208557455155No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49020.41263668274451376No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48020.4042189617582935No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44350.37332592573886547No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44040.37071643223313716No Hit
GCTTCATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT43440.3656657996414051No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAA42610.3586790912228423No Hit
GTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCG40210.3384765608559138No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39880.3356987129304612No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT39460.3321632701162487No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39320.3309847891781779No Hit
ACTTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAA38840.3269442831047922No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTG34430.2898221335555612No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32890.27685884323678206No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32810.2761854255578845No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32460.2732392232127074No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32320.27206074227463656No Hit
CAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCG31750.26726264131249106No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31510.2652423882757982No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31030.2612018822024125No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30920.2602759328939283No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29570.248912009562531No Hit
CTCCTAACCTCCTGATCTCTGATGCATCCACTTTACAAAGTGGGGTCCCA29270.24638669326666499No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29040.24445061743983434No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28840.2427670732425903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28200.23737973181140937No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28120.23670631413251178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27230.2292145424547758No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27030.22753099825753176No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT26530.22332213776442167No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26070.21944998611076036No Hit
TCTCTGATGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACT25750.2167563153951699No Hit
AGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAA25410.21389429025985507No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24010.20210948087914676No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC23980.20185694924956016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23890.20109935436080037No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTGGATGCATCAGAGATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTG23770.20008922784245392No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23540.1981531520156233No Hit
TTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGA23150.1948702408309974No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22930.19301834221402897No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22630.19049302591816292No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC22040.185526570536293No Hit
TACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCAT21930.18460062122780876No Hit
GGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAATTCCTA21710.18274872261084033No Hit
GGGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGGCTG21390.18005505189524987No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21350.17971834305580106No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21330.17954998863607669No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20990.1766879635007618No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGACGATTTTG20320.17104809043999428No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCTGCAGCTTCATACTTAC20210.17012214113151006No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20100.16919619182302584No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19730.16608163505812434No Hit
GGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTCCTAACCTCCTGATCTCTGATGCATC19670.16557657179895116No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19560.16465062249046694No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19350.16288290108336068No Hit
GACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18870.158842395009975No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18840.1585898633803884No Hit
CATCAGAGATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAATTCCTAATGTCCTGA18660.15707467360286878No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18310.1541284712576917No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACT18100.15236074985058545No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18080.15219239543086105No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18000.15151897775196344No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17980.15135062333223903No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17890.15059302844347922No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17850.1502563196040304No Hit
GAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTCCTAACCTCC17740.1493303702955462No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17680.14882530703637298No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17680.14882530703637298No Hit
CTCACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCT17470.14705758562926674No Hit
CACCATCACTTGCCGGGCCAGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTC17360.14613163632078252No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17220.14495315538271167No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17090.14385885165450307No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17050.14352214281505427No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16980.14293290234601885No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG16960.14276454792629445No Hit
ATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGA16870.14200695303753463No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC16590.13964999116139296No Hit
GGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATT16540.13922910511208195No Hit
GATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCGCTGAACCTTGATG16220.1365354343964915No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16150.13594619392745608No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16130.13577783950773167No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15500.13047467528641296No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGG15470.13022214365682633No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15210.1280335362004091No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGTAAGTATGAAGCTGCAGACAGTAATAAGTTGCAAAATCGTCAGG15090.12702340968206266No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15070.12685505526233828No Hit
GCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGA15060.12677087805247608No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14910.12550821990454303No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC14870.12517151106509425No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14420.12138353662129515No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14330.12062594173253534No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAA14280.12020505568322432No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14080.11852151148598028No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13900.11700632170846065No Hit
CACAATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCTGCAGCTTCATACTTACCCTCTCACTTT13820.11633290402956303No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13820.11633290402956303No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13810.11624872681970083No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGG13810.11624872681970083No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13530.11389176494355918No Hit
GTTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACAATCAGCAGCC13240.11145062585755533No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13220.11128227143783091No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGC13160.1107772081786577No Hit
GCTGATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCCGCTGAACCTTG13160.1107772081786577No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAA13160.1107772081786577No Hit
GGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGCCCCAGATCCACTGCC13080.1101037904997601No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12970.10917784119127587No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12890.10850442351237825No Hit
TCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGC12880.10842024630251605No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT12840.10808353746306724No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG12840.10808353746306724No Hit
GTCAGGACATTAGGAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAACTCCTAACCTCCTGATCTCTGATG12790.10766265141375624No Hit
GTCTGCAGCTTCATACTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCAC12790.10766265141375624No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12770.10749429699403183No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12600.10606328442637442No Hit
GGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC12560.10572657558692561No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12510.1053056895376146No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC12440.10471644906857916No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA12390.10429556301926816No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG12250.10311708208119734No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGT12240.10303290487133512No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA12170.10244366440229971No Hit
GGACCCCACTTTGTAAAGTGGATGCATCAGAGATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGG12090.10177024672340211No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA12050.1014335378839533No Hit
CTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATTCACTCTCACAATCAGCAGC11950.10059176578533129No Hit
GATCAGGAGGTTAGGAGTTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT11940.10050758857546907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38250.035.1753161
GTACCGG400.003049607433.3861
GACCGGA850.00318560233.0870770
TACGTAT850.00318560233.0870770
GTGGTAT4550.033.019121
CGGGAAT851.6118611E-731.4221217
AACGCAG43900.030.9289346
TATCAAC43050.030.927482
CAACGCA43850.030.7372555
ATCAACG43650.030.7239383
TCAACGC43800.030.6935924
GTATAAA450.00542755529.6764451
CGCAGAG45550.029.6620888
ACGCAGA46400.029.3344027
GCAGAGT46500.028.8396429
AGAGTAC46400.028.75800711
CAGAGTA47100.028.54428710
TACGGGT3300.028.31916215
GGAGTCG500.006969711328.19532848
TCTCGCG1754.7874437E-628.1240170
Produced by FastQC (version 0.11.5)