FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764666
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCCCGGCTCTCAGGCTGTTCATCTGCAGATACA88940.5040047238400921No Hit
GTATTACTGTCAGCAGTATGGTAGCTCTCTGTGGACGTTCGGCCAAGGGA83740.474537391211708No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63530.3600114695925461No Hit
GTATATTACTGTGCGAAAGATGGAGATTTTTACGATCTTTTGACTGGTCTAATAAGGGGGGGTTACTACTTTGA58700.3326408510165663No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCAGACCCACTGCCA51100.28957321102123573No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44630.25290904907784245No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCA43660.2474122581837016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42270.23953541350034513No Hit
GTGTTAACAGCGACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTACAT40870.23160190086962631No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39610.22446173950197942No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCTCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA38830.2200416396077218No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT37860.21454484871358095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37290.21131477571393115No Hit
GTATGGTAGCTCTCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT36980.20955806934570054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34630.19624110171556544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33410.18932761213736765No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC32580.18462417250629864No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30690.17391393045482828No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAGAAGTTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG29600.16773712419234008No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28470.16133364614040277No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT28460.1612769781930405No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27720.157083550088232No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27240.1543634886148427No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCGACTACTTAGCCTGGTACC27240.1543634886148427No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26920.15255011429924983No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26740.15153009124672884No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26650.15102007972046835No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCAGACCCACTGCCA26640.15096341177310607No Hit
GTCCTAGAGGCAGTGGTGGTAAGACATACTACGCAGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACGATT26460.14994338872058507No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26370.1494333771943246No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG26290.1489800336154264No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG26050.14762000287873173No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25770.14603330035258796No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25360.14370991451073462No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24870.14093318508998304No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA23990.13594640572210265No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23620.1338496916696984No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23490.1331130083539888No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23370.13243299298564146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23370.13243299298564146No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC23230.1316396417225696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23080.13078962251213544No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA22840.12942959177544078No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21660.12274277398669209No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTC21520.12194942272362023No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21360.12104273556582379No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG21260.12047605609220102No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG20650.11701931130310211No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTAC20450.11588595235585658No Hit
GAGTGTTAACAGCGACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG20310.1150926010927847No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20230.11463925751388648No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG20150.11418591393498825No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG20100.11390257419817688No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20090.11384590625081462No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19880.11265587935620677No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19870.1125992114088445No Hit
CAGTAATATACGGCCGTGTCCCCGGCTCTCAGGCTGTTCATCTGCAGATA19780.112089199882584No Hit
CCTCATGTATGGTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACGGACTT19780.112089199882584No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19740.11186252809313489No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC19140.10846245125139828No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACGGTGACCAGGGATCCCTGGCCCCAGGAGTCAAAGTAGTAACCCC18930.10727242435679048No Hit
GTCTAATAAGGGGGGGTTACTACTTTGACTCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCGCAGCATCCCCGA18830.10670574488316768No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCT18750.10625240130426948No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC18570.10523237825174848No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18560.1051757103043862No Hit
GGGCTGGAGTGGGTCTCAACTATGAGTCCTAGAGGCAGTGGTGGTAAGACATACTACGCAGACTCTGTGAAGGGC18500.10483570262021255No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18270.10353233983088019No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT18090.10251231677835919No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18060.10234231293627236No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17990.10194563730473642No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17850.10115228604166454No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17790.10081227835749088No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17720.10041560272595494No Hit
CTGTTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA17720.10041560272595494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG25300.048.45349570
GGTATCA13950.041.548641
GTGGTAT4300.039.644561
GTATCAA38300.038.812341
TGGTATC4350.036.8229262
TATCAAC41150.036.10992
TACGTAT951.5863504E-435.4503670
AATGGTA7800.035.40490770
TCAACGC43400.034.4665874
ATCAACG43550.034.3478743
CAACGCA44250.033.9585235
AACGCAG44800.033.39146
AACTGTG35150.033.342570
ATTACCG552.6061185E-432.57964763
CGCAGAG47000.032.0459028
AGAGTAC47700.031.21578211
GTTCATC23600.031.09508134
GCAGAGT48600.030.9882589
TCGTCTC24550.030.5675865
TGAGTAC450.004913002730.2880274
Produced by FastQC (version 0.11.5)