FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034730
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACAGTGCTGATACTTGTATATGAAGCGCAGATATAAT41260.39875136509041004No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT34620.33458003537154624No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33840.3270418370009568No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCACCATCTCCTG32330.31244865810404643No Hit
TCTCTGGACTCCAGGCTGAGGACGCGGCTGATTATATCTGCGCTTCATAT31920.3084862717810443No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31510.3045238854580422No Hit
GATTATATCTGCGCTTCATATACAAGTATCAGCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGT30700.29669575638089163No Hit
GATATAATCAGCCGCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGG30240.29225015221362094No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30200.2918635779382061No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC28930.27958984469378484No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACAGTGCTGATACTTGTATATGAAGCG26740.25842490311482225No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTAGCCAGACTTGGAGCCAGAGAAG26680.2578450417016999No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26450.25562223961806463No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25650.24789075410976777No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATGACTTTGTCTCCTGGTACCAACAGCAGTCGGGCAAAGC25310.2446048727687416No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATG24670.23841968436210415No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24490.23668010012273732No Hit
GTGCTGATACTTGTATATGAAGCGCAGATATAATCAGCCGCGTCCTCAGC23880.230784842422661No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23720.2292385453210016No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22530.21773796062741005No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21720.20990983155025947No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20980.2027582074550849No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20970.2026615638862312No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20930.20227498961081633No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20770.20072869250915698No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCAC20570.19879582113208277No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20420.1973461675992771No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20370.19686294975500856No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19060.18420264223517246No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19030.18391271152861133No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18710.18082011732529257No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18320.17705101813999788No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18050.17444164178094768No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18020.17415171107438657No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17870.17270205754158088No Hit
GACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCACCATCT17400.1681598098054565No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17220.1664202255660897No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17200.1662269384283823No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT17170.16593700772182116No Hit
CTTGTATATGAAGCGCAGATATAATCAGCCGCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTAG16810.16245783924308757No Hit
TATCAGCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA16510.15955853217747626No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCAC16320.15772230436925574No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16100.1555961458544741No Hit
ATATACAAGTATCAGCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC15250.1473814425019087No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG15220.14709151179534757No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC15000.14496535328056592No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14800.14303248190349174No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14720.14225933335266203No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAAC14590.1410029669575638No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14380.13897345201163588No Hit
GCTTATGACTTTGTCTCCTGGTACCAACAGCAGTCGGGCAAAGCCCCCAAACTCATTATTTCTGAAGTCGATTAC14240.13762044204768392No Hit
GGAACCAACAGTGACGTTGGTGCTTATGACTTTGTCTCCTGGTACCAACA14240.13762044204768392No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCG14180.13704058063456168No Hit
CCACAGTGCTGATACTTGTATATGAAGCGCAGATATAATCAGCCGCGTCC13950.13481777855092633No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13870.13404463000009664No Hit
GTACCAACAGCAGTCGGGCAAAGCCCCCAAACTCATTATTTCTGAAGTCG13580.13124196650333902No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13380.12930909512626482No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13030.12592657021638495No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12640.12215747103109023No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12530.12109439177369942No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12460.12041788679172344No Hit
GCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCC12390.11974138180974747No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12350.11935480753433261No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12270.11858165898350295No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT12260.11848501541464923No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC12130.11722864901955099No Hit
GCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTG12090.11684207474413615No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12090.11684207474413615No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11960.1155857083490379No Hit
GTCCAGGGGACCCTGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11770.11374948054081742No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTC11740.11345954983425627No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA11510.11123674775062095No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA11500.11114010418176723No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11350.10969045064896156No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT11210.10833744068500961No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11140.10766093570303363No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11070.10698443072105768No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11040.10669450001449654No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11030.10659785644564282No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10860.10495491577512973No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10770.10408512365544635No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10760.10398848008659263No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10760.10398848008659263No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10610.10253882655378699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA100.009563587131.1850770
AGGGGGG3300.065.5925470
TCGTATG2900.052.28811340
AGAACCG200.0078220350.95705830
AACGTAC200.00792296350.7920615
CGTATGC2950.050.33019341
ACGAGAC3000.049.67054722
TCTCGTA3050.048.39146838
GCCGTCT3150.047.57776646
ATCTCGT3150.046.77731737
CGAGACG3200.046.5706423
TATGCCG3200.046.57027443
GGTATCA9000.044.7852441
ATGCCGT3400.043.8959844
TACGTAT1208.76571E-943.72835570
CATCTCG3400.043.2827736
CTTATAC3450.043.1980481
TCCGAGC3450.043.1834313
ATCTCCG3450.043.18134310
CACGAGA3500.042.57269721
Produced by FastQC (version 0.11.5)