FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034730
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACAGTGCTGATACTTGTATATGAAGCGCAGATATAAT37480.3622200960637075No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCACCATCTCCTG35000.3382524909879872No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32990.3188271336483914No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31740.30674668754167755No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31710.3064567568351164No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT31220.3017212219612846No Hit
TCTCTGGACTCCAGGCTGAGGACGCGGCTGATTATATCTGCGCTTCATAT30370.2935065186087192No Hit
GATTATATCTGCGCTTCATATACAAGTATCAGCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGT30130.29118707295623014No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29390.28403544886105553No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCACAGTGCTGATACTTGTATATGAAGCG26740.25842490311482225No Hit
GATATAATCAGCCGCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGG26650.25755511099513884No Hit
GTGCTGATACTTGTATATGAAGCGCAGATATAATCAGCCGCGTCCTCAGC26520.2562987446000406No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26030.25156320972620877No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC25630.24769746697206035No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATGACTTTGTCTCCTGGTACCAACAGCAGTCGGGCAAAGC25330.24479815990644904No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTAGCCAGACTTGGAGCCAGAGAAG25170.24325186280478966No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATG24740.2390961893440801No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24650.2382263972243967No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24430.23610023870961508No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23590.22798217892590336No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23530.22740231751278112No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23020.2224734955012419No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22610.2185111091782397No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21620.20894339586172236No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCAC21450.20730045519120932No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19880.19212741488117674No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19520.18864824640244315No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19220.18574893933683184No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18890.1825597015646594No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18880.18246305799580567No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17550.16960946333826216No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17540.16951281976940843No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17460.16873967121857877No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17290.16709673054806568No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17160.16584036415296743No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16840.1627477699496487No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16700.16139475998569677No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16690.16129811641684305No Hit
CTTGTATATGAAGCGCAGATATAATCAGCCGCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTAG16350.15801223507581688No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16150.15607936369874267No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16070.15530621514791298No Hit
GACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCACCATCT15790.1526001952200091No Hit
TATCAGCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA15730.15202033380688681No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG15390.14873445246586064No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14950.1444821354362974No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCAC14870.1437089868854677No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14760.14264590762807688No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAAC14370.1388768084427822No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14260.13781372918539136No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13850.13385134286238923No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13760.13298155074270582No Hit
ATATACAAGTATCAGCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC13660.13201511505416874No Hit
GCTTATGACTTTGTCTCCTGGTACCAACAGCAGTCGGGCAAAGCCCCCAAACTCATTATTTCTGAAGTCGATTAC13310.12863259014428885No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13270.12824601586887402No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCG13130.12689300590492206No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13090.12650643162950723No Hit
GTACCAACAGCAGTCGGGCAAAGCCCCCAAACTCATTATTTCTGAAGTCG12990.12553999594097012No Hit
GGAACCAACAGTGACGTTGGTGCTTATGACTTTGTCTCCTGGTACCAACA12980.1254433523721164No Hit
GCCTGCCTCCGTGTCAGGGTCCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTG12610.12186754032452911No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12560.12138432248026056No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12330.11916152039662521No Hit
CCACAGTGCTGATACTTGTATATGAAGCGCAGATATAATCAGCCGCGTCC12260.11848501541464923No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12020.11616556976216016No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11910.11510249050476935No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11760.1136528369719637No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTC11760.1136528369719637No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11640.11249311414571916No Hit
GCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCC11510.11123674775062095No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT11140.10766093570303363No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA11100.1072743614276188No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11100.1072743614276188No Hit
GTATCAGCACTGTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA11000.1063079257390817No Hit
GTCCAGGGGACCCTGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC10840.10476162863742233No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10700.10340861867347037No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC10700.10340861867347037No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10660.10302204439805553No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10650.10292540082920182No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10480.10128246015868873No Hit
GCCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTAGCCAGACTTGGAGCC10430.10079924231442018No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT10380.10031602447015163No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10370.10021938090129792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA200.00793544550.7718325
GGTATCA8400.041.4959221
AATGGTA9550.039.67866570
GTATCAA24000.037.6484831
TATCAAC25800.034.8771742
TGGTATC2450.034.518612
ATCAACG26300.034.1992233
TCAACGC26450.033.8757974
GCGGCAT800.002855613633.8331570
CAACGCA27250.032.879695
AACGCAG27550.032.5216526
GTGGTAT2650.031.9257871
TACGGGC2050.031.34541915
GTGGTGA7800.031.23059870
GTACTAT553.666143E-430.7365886
CGCAGAG29250.030.6315048
CAGAGTA29550.030.31759510
AGAGTAC29700.030.16738911
ACGCAGA30450.029.646427
GCAGAGT30250.029.5056959
Produced by FastQC (version 0.11.5)