FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798711
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44220.5536420557623472No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGCCTGCATGTGAACTG42010.5259724731473587No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG36440.4562351088190848No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGCCTGCATGTGAACTGCAGTAGTAAT36280.4542318811184521No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31470.3940098483681832No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC29670.371473536736066No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29510.3694703090354334No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29060.36383623112740404No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT25600.3205164321012231No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23940.2997329447071594No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG23590.29535088411202554No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23370.2925964460236556No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23020.2882143854285217No Hit
GATTACTACTGCAGTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGT21420.26818210842219525No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATCACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC21230.265803275527694No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20880.2614212149325601No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20630.2582911716503216No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19790.2477742262220002No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATCACTATGTCTCCTGGTACCAACAACA19320.2418897448513918No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19060.23863449983786375No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18130.22699073882793652No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17950.22473710766472477No Hit
CTACTGCAGTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC17920.22436150247085615No Hit
CCTGAGGGCCGCTCAGTGACCTCATAAATGATGAGTTTGGGGGCTTTGCC17920.22436150247085615No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17850.2234850903518294No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17720.22185746784506538No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17670.22123145918861767No Hit
ACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17250.21597298647445698No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17110.2142201622364034No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCA16690.20896168952224273No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTGATAA16470.2062072514338728No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16420.20558124277742512No Hit
CCCCTGAGGGCCGCTCAGTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA16130.20195039257002845No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16090.2014495856448703No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15690.1964415163932887No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15690.1964415163932887No Hit
GTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGACGGTCAGGGAGG15680.19631631466199914No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15540.19456349042394558No Hit
CTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC15500.1940626834987874No Hit
CTGCAGTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG15370.1924350609920234No Hit
GGATGAGGCTGATTACTACTGCAGTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGAC15220.1905570350226803No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15110.18917981597849537No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGC15080.18880421078462672No Hit
GTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGCCTGCATGTGAACTGCAGTAGTAATCAGCCTCATCCTCAG15040.18830340385946856No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT15020.1880530003968895No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA14370.1799148878630694No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14050.17590843246180407No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13790.17265318744827604No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCACTGGGCTCCAGG13720.17177677532924923No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13430.16814592512185259No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTG13330.1668939078089572No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTC13270.16614269742121993No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13150.16464027664574546No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT13130.1643898731831664No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGCAGTTCACAT13020.16301265413898142No Hit
GCTCCAGGCTGAGGATGAGGCTGATTACTACTGCAGTTCACATGCAGGCA12990.1626370489451128No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT12940.16201104028866511No Hit
GCCTGGAGCCCAGTGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12850.16088422470705926No Hit
CATTGGTGCTTATCACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGT12840.16075902297576972No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTC12740.1595070056628743No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12720.15925660220029522No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGACGGTCAGGGAGGCCGTGTTGCC12550.15712817276837304No Hit
GGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCT11950.14961606889100063No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11740.14698683253392028No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11700.14648602560876212No Hit
GGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11510.14410719271426084No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11410.14285517540136547No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11400.14272997367007592No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11380.14247957020749683No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11340.14197876328233866No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10970.13734629922462568No Hit
CTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC10940.13697069403075707No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10930.13684549229946752No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10800.13521786979270348No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC10800.13521786979270348No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10790.13509266806141396No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10760.1347170628675453No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10730.13434145767367672No Hit
ATACCAAAGTGTTCCTGCCTGCATGTGAACTGCAGTAGTAATCAGCCTCA10660.13346504555464994No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10630.13308944036078132No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10600.13271383516691268No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10300.12895778322822649No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10280.1287073797656474No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG10070.12607814340856704No Hit
CGTCACCACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC9970.12482612609567166No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9960.12470092436438211No Hit
GCCCAGTGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCT9840.12319850358890763No Hit
CCCTGACCGTCACTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGC9730.12182128454472267No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9700.12144567935085405No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT9590.12006846030666912No Hit
TTTATGAGGTCACTGAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC9370.11731402221829923No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC9340.11693841702443061No Hit
TCACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCACTGAGCGGCC9250.11581160144282475No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC9120.11418397893606072No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9090.1138083737421921No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC8920.11167994431026992No Hit
TCCTCAGCCTGGAGCCCAGTGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC8860.11092873392253269No Hit
CCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTGATAAGCACCAATGT8810.11030272526608498No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8810.11030272526608498No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGA8800.11017752353479544No Hit
CTCCCTGACCGTCACTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGCAGTTCACATGCAGGCAGGAACA8750.10955151487834773No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8720.10917590968447911No Hit
GTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTG8640.1081742958341628No Hit
GCCCCCAAACTCATCATTTATGAGGTCACTGAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT8630.10804909410287325No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8580.10742308544642555No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8540.10692227852126739No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC8530.10679707678997787No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCACTGAGCGGCCCTCAGGGGTCCCTGATCG8500.10642147159610922No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATCACTA8320.10416784043289751No Hit
GGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGAC8230.10304102485129164No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8190.10254021792613349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)