FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798711
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39710.4971760749507644No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGCCTGCATGTGAACTG39350.49266881262434103No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG35770.4478465928226856No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGCCTGCATGTGAACTGCAGTAGTAAT34530.43232157814278255No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34090.42681270196604276No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29020.36333542420224585No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC26920.3370430606314424No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC25910.3243976857711989No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25460.3187636078631695No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT24640.3084970658974272No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22940.28721277157820535No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG22470.281328290207597No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21630.27081134477927565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21610.27056094131669656No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATCACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC21380.2676813014970371No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20920.2619220218577183No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20630.2582911716503216No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19680.24639700717781526No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19540.24464418293976167No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATCACTATGTCTCCTGGTACCAACAACA18790.23525405309304617No Hit
GATTACTACTGCAGTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGT18680.23387683404886123No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18670.2337516323175717No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18480.2313727994230704No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17890.22398589727698753No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17650.22098105572603857No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17160.2148461708928511No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16800.21033890856642767No Hit
CCTGAGGGCCGCTCAGTGACCTCATAAATGATGAGTTTGGGGGCTTTGCC16460.2060820497025833No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTGATAA16260.20357801507679246No Hit
CTACTGCAGTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC16180.20257640122647613No Hit
ACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16030.20069837525713308No Hit
CCCCTGAGGGCCGCTCAGTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA16020.2005731735258435No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15580.19506429734910374No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15580.19506429734910374No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15570.1949390956178142No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCA15540.19456349042394558No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15410.19293586791718156No Hit
GTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGCCTGCATGTGAACTGCAGTAGTAATCAGCCTCATCCTCAG15380.19256026272331295No Hit
GGATGAGGCTGATTACTACTGCAGTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGAC15290.19143344714170707No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15250.1909326402165489No Hit
GTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGACGGTCAGGGAGG15220.1905570350226803No Hit
CTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14810.18542376403980915No Hit
CTGCAGTAGTAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG14740.18454735192078237No Hit
GGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCT14540.18204331729499154No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT14230.1781620636250158No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14040.17578323073051455No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA13600.17027435455377476No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13550.16964834589732708No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13530.16939794243474798No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13450.16839632858443165No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13160.164765478377035No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13110.1641394697205873No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13100.16401426798929775No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTC12820.16050861951319062No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12790.160133014319322No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12780.16000781258803246No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCAAAGTGTTCCTGC12700.15900619873771615No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12600.15775418142482076No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCACTGGGCTCCAGG12410.15537534853031948No Hit
GCCTGGAGCCCAGTGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12330.15437373468000315No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTC12300.15399812948613453No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTG12170.15237050697937052No Hit
GGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12130.15186970005421235No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12080.15124369139776464No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGACGGTCAGGGAGGCCGTGTTGCC11980.14999167408486924No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11830.14811364811552613No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGCAGTTCACAT11830.14811364811552613No Hit
CATTGGTGCTTATCACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGT11800.14773804292165751No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11580.14498360483328762No Hit
GCTCCAGGCTGAGGATGAGGCTGATTACTACTGCAGTTCACATGCAGGCA11390.14260477193878637No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11220.1404763425068642No Hit
CTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11000.1377219044184943No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10890.13634468537430935No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10870.13609428191173029No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG10730.13434145767367672No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10500.13146181785401728No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10450.1308358091975696No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC10450.1308358091975696No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10350.12958379188467417No Hit
CCCTGACCGTCACTGGGCTCCAGGCTGAGGATGAGGCTGATTACTACTGC10280.1287073797656474No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG10220.12795616937791016No Hit
CGTCACCACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC10200.1277057659153311No Hit
ATACCAAAGTGTTCCTGCCTGCATGTGAACTGCAGTAGTAATCAGCCTCA10190.12758056418404154No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9960.12470092436438211No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATAC9880.1236993105140658No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC9580.11994325857537957No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9460.1184408377999051No Hit
GCCCAGTGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCT9350.11706361875572016No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9300.11643761009927245No Hit
GGCTGAGGATGAGGCTGATTACTACTGCAGTTCACATGCAGGCAGGAACA9300.11643761009927245No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9290.11631240836798291No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT9010.11280675989187579No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8990.11255635642929669No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC8960.11218075123542808No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8910.11155474257898038No Hit
GGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGAC8640.1081742958341628No Hit
TCACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCACTGAGCGGCC8600.10767348890900463No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGA8590.10754828717771509No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATCACTA8490.1062962698648197No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8460.10592066467095107No Hit
GTGTTGTTGGTACCAGGAGACATAGTGATAAGCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA8430.10554505947708245No Hit
TTTATGAGGTCACTGAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC8430.10554505947708245No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC8410.10529465601450338No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8360.10466864735805567No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC8330.10429304216418704No Hit
GTTCACATGCAGGCAGGAACACTTTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTG8280.10366703350773936No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG8250.10329142831387071No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8240.10316622658258119No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8200.10266541965742303No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTACTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC8170.1022898144635544No Hit
GCCCCCAAACTCATCATTTATGAGGTCACTGAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT8150.10203941100097533No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT8100.10141340234452761No Hit
CCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTGATAAGCACCAATGT8000.1001613850316322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAC200.005743520355.09480766
ATCGGTG200.00782886750.9447326
GGTATCA4700.046.2302971
GTGGTAT1200.045.2671661
TGTAAGG306.8446656E-445.247312
AATGGTA3600.044.07003470
TGGTATC1400.041.207372
TCAACAG350.001465902338.7591175
GATTGAC558.252377E-637.0367741
GTATCAA10700.034.9022561
ACGGGTA400.002818888833.92485416
TATCAAC12050.030.9784492
GCTTGAC553.577607E-430.863981
TCAACGC12250.030.1767434
TGCGTCA450.005027156330.14409410
ATCAACG12350.029.9380233
CAACGCA12450.029.6919775
GACAGGT1152.0208972E-929.5220661
AACGCAG12700.029.1056656
ATGGCAA1000.00622981528.84583770
Produced by FastQC (version 0.11.5)