FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624184
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACACTCGTGTCCTCTGCTCTCAGGCTGCTCATATGCAGATACA11950.1914499570639427No Hit
GTCTACTACTGTGCGAGAGTGTGGCGGACTACGGTGACTACTGGCTATTC8900.14258616049113723No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8080.12944900862566167No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8050.12896838111838815No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC7680.12304064186201505No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC7330.11743332094382426No Hit
GTATTACTGTCAGCAGTATGGTAGCGCACTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA7280.11663227509836843No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT7150.11454955590018327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6930.11102495418017765No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6890.11038411750381298No Hit
CTCCTGCAGGGCCAGTGAGACTTTTGGCAGCAATTACTTAGCCTGGTACC6560.10509721492380451No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGACTTTTGGCAG6320.10125219486561655No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6290.10077156735834306No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA6280.10061135818925189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)