FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801564
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62910.34919658696554773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60280.3345981602651918No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47390.26304921723569075No Hit
GTATCAGCACAAAGCGGGGAAAGTCCCCAAGCTCCTGATCTATGGTGCGTCCACTTTGCAGTCAGGGGTCCCAT45260.25122615682817817No Hit
CCATAGATCAGGAGCTTGGGGACTTTCCCCGCTTTGTGCTGATACCATGC44050.2445097703994973No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43350.24062425758951664No Hit
GATCTATGGTGCGTCCACTTTGCAGTCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA42860.23790439862253018No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41850.2322981587109867No Hit
GTGCTGATACCATGCTAAATAGTTGTTAATACCCAGACTCGCCCGGCAAG39770.22075263493275843No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39520.21936495178633675No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC35450.19677347016259206No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34790.19310998665603887No Hit
GTGTACGGTGGGACCTTCAGTGGTCACTACTGGAGCTGGGTCCGCCAGCC34620.19216636211647214No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34390.1908896936217642No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34180.18972403977877No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34070.18911345919434447No Hit
GTAATAAGTTGCGACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA33340.1850614244067932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32630.1811204042709557No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG31350.17401546656127675No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31170.17301633469585317No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31120.17273879806656883No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCTGGGTATTAACAA30810.17101807096500596No Hit
ACTTATTACTGTCAAAAATATAACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA30310.16824270467216262No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT30170.1674656021101665No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29380.16308052336747403No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28440.15786283473692858No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT28360.15741877613007366No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT28170.1563641369387932No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28020.15553152705094017No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC27980.1553094977475127No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCTGGGTATTAACAA27880.15475442448894405No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27630.15336674134252237No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27100.15042485307210846No Hit
ATATAACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26720.14831557468954754No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG26490.14703890619483959No Hit
CTGTTATATTTTTGACAGTAATAAGTTGCGACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC25750.14293136408143145No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT25030.13893483661973707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGCA24760.1374361388216017No Hit
GAAGTACACAGCCGTGTCCGCGGCGGTCACGGAGGTCAGGTTCAGGGAGA24400.13543787509075447No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC22690.12594612236923028No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG22270.1236148146832419No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCT22100.12267119014367515No Hit
GTTATATTTTTGACAGTAATAAGTTGCGACATCTTCAGGCTGCAGGCTGC21860.12133901432311035No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21860.12133901432311035No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21670.1202843751318299No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20960.11634335499599237No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20700.11490016452371385No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTCGCAACTTATTACTGTCAAAAATATAACAGTGCC20390.11317943742215096No Hit
CTACTGGAGCTGGGTCCGCCAGCCCCCAGGCAAGGGGCTGGAGTGGATTGGGGAAATCGCTTATGGTGGAAGCAC20340.11290190079286663No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20320.1127908861411529No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACC20280.11256885683772544No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT19610.10884986600531539No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19190.106518558319327No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGTATCTGGGAGCCAGAG19140.10624102169004267No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19100.10601899238661518No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19040.10568594843147398No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18830.10452029458847978No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT18590.10318811876791499No Hit
AAATATAACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18510.10274406016106007No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18230.10118985503706779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG150.002719747866.544287
CGTACTA3550.046.1812729
TCGTATG3600.043.89216240
GTACTAG4000.041.88427430
CTAGATC3800.041.67050633
CGTATGC3850.041.19191741
TACTAGA4150.039.64386731
GACCGTA4100.039.04160326
ATCTCGT3950.038.73600437
ACTAGAT4200.038.4273832
ACGAGAC4200.038.0443122
ATCTCCG4300.037.91581710
CCGTCTT4300.037.6299947
TAGATCT4300.036.89768234
GATCTCG4250.036.6955736
GTATGCC4600.036.09312442
TGCTTGA4800.035.58747555
CTTATAC4550.035.124651
GTATCAA41900.035.043441
GAGACCG4700.034.72170624
Produced by FastQC (version 0.11.5)