FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801564
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59450.3299910522190719No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54460.30229289661649544No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50220.278757790453184No Hit
GTATCAGCACAAAGCGGGGAAAGTCCCCAAGCTCCTGATCTATGGTGCGTCCACTTTGCAGTCAGGGGTCCCAT47210.26205008537026714No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45780.25411253777273524No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43110.23929208176895186No Hit
GTGCTGATACCATGCTAAATAGTTGTTAATACCCAGACTCGCCCGGCAAG42300.23479598837454568No Hit
GATCTATGGTGCGTCCACTTTGCAGTCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA41100.22813510927172168No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39750.2206416202810447No Hit
CCATAGATCAGGAGCTTGGGGACTTTCCCCGCTTTGTGCTGATACCATGC38820.2154794389763561No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC34800.19316549398189573No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34680.19249940607161334No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34510.19155578153204658No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34390.1908896936217642No Hit
GTGTACGGTGGGACCTTCAGTGGTCACTACTGGAGCTGGGTCCGCCAGCC34230.19000157640805432No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32970.18300765335008914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32970.18300765335008914No Hit
GTAATAAGTTGCGACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA32610.18100938961924196No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31700.1759582229662671No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31100.1726277834148551No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT30700.17040749038058045No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCTGGGTATTAACAA29400.1631915380191878No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG29070.16135979626591118No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28110.156031092983652No Hit
ACTTATTACTGTCAAAAATATAACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA28010.15547601972508332No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCTGGGTATTAACAA27560.15297819006152433No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26660.14798253073440634No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26620.14776050143097888No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC26470.14692789154312585No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT26460.146872384217269No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26000.14431904722785313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25960.14409701792442567No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25540.14176571023843726No Hit
CTGTTATATTTTTGACAGTAATAAGTTGCGACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC25150.1396009245300195No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT24770.13749164614745854No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG24380.13532686043904074No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24090.13371714798919163No Hit
ATATAACAGTGCCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24020.13332859670819353No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCT23470.13027569378606588No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGCA23410.12994264983092468No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22920.12722279086393823No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC22870.1269452542346539No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22410.12439191724523803No Hit
GAAGTACACAGCCGTGTCCGCGGCGGTCACGGAGGTCAGGTTCAGGGAGA21990.12206060955924963No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTCGCAACTTATTACTGTCAAAAATATAACAGTGCC21480.11922973594054942No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG21010.1166208916252767No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20400.11323494474800783No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20190.11206929090501364No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19980.11090363706201943No Hit
GTTATATTTTTGACAGTAATAAGTTGCGACATCTTCAGGCTGCAGGCTGC19860.11023754915173704No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18940.10513087517290533No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGTATCTGGGAGCCAGAG18920.1050198605211916No Hit
CTACTGGAGCTGGGTCCGCCAGCCCCCAGGCAAGGGGCTGGAGTGGATTGGGGAAATCGCTTATGGTGGAAGCAC18320.10168942096977959No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18250.10130086968878152No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACC18230.10118985503706779No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18200.10102333305949719No Hit
GTGTACTTCTGTGCGAGAGGCTCGTCGTTAATTACTGCCCTTGATTATAGCTACTTCGGTTTGGACGCCTGGGGC18170.1008568110819266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG200.00854232649.8354535
GTATCAA46900.035.5880131
TATCAAC50400.032.635792
ATCAACG50900.032.4376643
TCAACGC51050.032.3450474
CAACGCA51300.032.3169445
AACGCAG51450.032.2200436
GTGGTAT6050.031.8746551
CGCAGAG53850.030.5998278
ACGCAGA54350.030.500857
AGAGTAC54550.030.08118411
CAGAGTA54900.030.07426310
AATGGTA14800.029.8944770
GCAGAGT55550.029.6625569
GCGTACG450.00556145929.52965710
TGTTAGA21850.028.79138470
TTTCGCA550.007363493627.8803365
TTCGCAC756.791678E-527.21058537
AACAGAT27750.027.15414270
GATTCAC3350.026.7973481
Produced by FastQC (version 0.11.5)