FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547160
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCCCGGCTCCCAGGCTGTTCATTTGAAGATACA60040.3880658755396985No Hit
GTATAGTAAGTGGCCTTCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT54490.3521936968380775No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48910.3161276144677991No Hit
GAGTATTACCAGCGGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGACGCATC46820.3026189922179994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41780.2700431758835544No Hit
GTATATTACTGCGCAAAAGCCCGGTTGCGTTGTAGTGGTGACAATTGCCA38320.247679619431733No Hit
CTTACTATACTGCTGACAATAATAAACTGCAGAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACT37370.24153933659091498No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA37010.23921249256702604No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31610.20430983220869206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30830.19926833682359937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30500.19713539646836784No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30330.19603660901264253No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT29090.18802192404146953No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28480.18407921611210215No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT28030.18117066108224103No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25760.1664986168204969No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTCTGCAGTTTATTATTGTCAGCAGTATAGTAAGTGG25150.16255590889112956No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24830.16048760309211718No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24600.15900100829907704No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24480.15822539362444737No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT24110.15583391504433933No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24000.1551229349259288No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23890.1544119548075183No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23220.1500814395408361No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22630.14626800072390703No Hit
CTCCTGCAGGGCCAGTCAGAGTATTACCAGCGGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT22420.14491067504330515No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22100.14284236924429278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21430.1385118539776106No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21390.13825331575273403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20980.13560329894774942No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20560.13288864758654567No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTACCAG20530.13269474391788827No Hit
GCAGTATAGTAAGTGGCCTTCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT20420.13198376379947777No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19960.12901057421339746No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19630.12687763385816594No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19470.12584348095865974No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC19380.1252617699526875No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18950.12248248403526463No Hit
CTATACTGCTGACAATAATAAACTGCAGAATCTTCAGACTGCAGGCTGGT18370.11873367977455467No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTACCAGCGGCTTAGCCTGGTA18290.11821660332480158No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA18150.11731171953773364No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18000.11634220119444659No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTCTGCAGTTTATTATTGTCAGCAGTATAGTAAGTGGCCTTCTTTCGG17780.1149202409576256No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17160.1109128984720391No Hit
GGATACCAGTGGCCCTGGTGGATGCGTCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG17040.11013728379740945No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16930.10942630367899896No Hit
CTACTGTGCAGCCTCTGGATTCACCTTCAGTAACTACGACATGCACTGGGTCCGCCAACCTACAGGAAAAGGC16910.10929703456656066No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATGACAGGAACTCCCTGTATCTTCAAATGAA16800.10858605444815017No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16500.10664701776157606No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT16410.10606530675560381No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15940.10302748261330438No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15900.10276894438842783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15670.10128234959538769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)