FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819319
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC98220.5398723368469192No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT94310.5183807787419359No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGGATAAAACACT86400.4749029719362025No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC76750.4218611469456428No Hit
ATTATAATACTCCCCCCCTTTTCGGCCCTGGGACCAAGGTGGAGATCAAA66080.3632128285363919No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65970.3626082066971213No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA63940.3514501854814906No Hit
GTTTTATCCAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA59430.3266606900713948No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG58060.319130399891388No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC57980.3186906749173729No Hit
CTCCTAAGGTTCTCATTGTCTGGGCATCTGCCCGGGAATCCGGGGTCCCT56610.311160384737366No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56390.30995114105882476No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG55610.30566382256217844No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT53190.2923621420982247No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51800.284721920674714No Hit
GTATTATAATAATGCTGACAGTAATAAACTGCCATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAAT51520.2831828832656615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT51200.28142398336960145No Hit
GTAATAAACTGCCATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA51190.2813690177478496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG50240.27614728368142144No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC49480.27196989642827896No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC45600.2506432351885513No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG45390.24948895713176192No Hit
GGATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA44910.2468506072876719No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT43850.24102425138197314No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATC43160.23723162348109372No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG41800.22975629892283872No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41550.22838215837904186No Hit
AACCAGGACAGCCTCCTAAGGTTCTCATTGTCTGGGCATCTGCCCGGGAA41070.22574380853495182No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG40800.22425973674765118No Hit
TTATAATACTCCCCCCCTTTTCGGCCCTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT40790.22420477112589932No Hit
GTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGACAATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA38960.21414606234530614No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGA38520.21172757498822362No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37140.20414231918646483No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCAGGGCCGAAAAGG36010.1979312039285029No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35720.19633720089769854No Hit
GTTTATTACTGTCAGCATTATTATAATACTCCCCCCCTTTTCGGCCCTGGGACCAAGGTGGAGATCAAACGAA35370.1944134041363829No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34920.1919399511575485No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC34790.19122539807477412No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG32500.17863827069359467No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32360.17786875198906846No Hit
GTCTGAAGATATGGCAGTTTATTACTGTCAGCATTATTATAATACTCCCC31200.1714927398658509No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC30340.16676569639518965No Hit
CAGTAATAAACTGCCATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA29970.16473196839037024No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGACAATAAGAACTA29740.16346775909007713No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA29520.16225851541153585No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT29380.1614889967070096No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28700.15775133442788208No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC28600.15720167821036332No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC28310.15560767517955895No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC28310.15560767517955895No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT27980.15379380966174705No Hit
TGCCCAGACAATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACC27910.15340905030948393No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG27750.1525296003614539No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27690.15219980663094268No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC27540.15137532230466455No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT27490.15110049419590518No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27470.15099056295240143No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27130.14912173181283767No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26910.1479124881342964No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26700.14675821007750703No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG26620.14631848510349202No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC26290.14450461958568014No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC26190.14395496336816138No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT25580.140602060441297No Hit
TCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC25430.13977757611501887No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24960.13719419189268073No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG24760.13609487945764323No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA24700.13576508572713197No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATATGGCAGTTTATTACTGTCAGCATTATTATAATACT24520.13477570453559823No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24230.13318170150479383No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24220.13312673588304194No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24200.1330168046395382No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24180.13290687339603444No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA24080.1323572171785157No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24050.1321923203132601No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG23930.13153273285223757No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTCATTGTCTGGGCATCTGCCCGGGAATC23040.12664079251632065No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22960.12620106754230567No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG22950.12614610192055378No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT22910.12592623943354628No Hit
GCTAAGTAGTTCTTATTGTCGGAGCTGGATAAAACACTCTGGCTGGACTT22820.1254315488377794No Hit
GTCTGGGCATCTGCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAG22740.12499182386376442No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGC22070.12130912720638876No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG22020.1210342990976294No Hit
ATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTA21580.11861581174054686No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTCATT21560.11850588049704311No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA21450.11790125865777248No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTC21400.11762643054901312No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT21390.11757146492726124No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21120.11608739313996061No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT20830.11449339010915624No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA20820.11443842448740436No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20800.11432849324390061No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20770.11416359637864497No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC20610.11328414643061496No Hit
GAAATACACGGCCGTGTCGTCGACTCTCAGGCTGTCCATCTGCAGAAATA20370.11196497150856996No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCAGGGCCGAAAAGGGGGGGAGTATT20300.11158021215630684No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20100.11048089972126934No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20010.10998620912550247No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19970.10976634663849495No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGACAAT19840.10905179355572057No Hit
CTCCTGTGCAGTCTCTGGCTTCAGCTTCAGTAATGATTTCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG19830.1089968279339687No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATATGGCAGTTTATTACTGTCAGCATTATTATAATACTCCCCCCCTTTT19520.10729289365966058No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19480.10707303117265307No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG19320.10619358122462305No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19140.1052042000330893No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18660.10256585018899929No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18640.10245591894549554No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC18580.10212612521498429No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18490.1016314346192174No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18270.10042219094067616No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18210.10009239721016491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.6825122E-460.1253970
TCGAAGC200.006974159752.45899648
GGTATCA10550.047.6407241
CGTATGC3000.044.81042541
TCGTATG3050.043.99424440
GCCGTCT3250.042.8697446
ACCGTAC3000.042.82399427
GTGGTAT2300.042.531891
TACTAGA3050.042.26756331
CGTACTA3050.042.1696129
GACCGTA3050.042.10339726
GTACTAG3150.041.94936830
TGCCGTC3350.041.49226445
ACGAGAC3200.041.11562722
ATGCCGT3400.040.7942744
ATCTCGT2600.040.77978537
CACGAGA3250.040.47862621
GATCTCG2650.039.939136
TCCGAGC3300.039.84778613
CGTTGAA350.001341210939.4691942
Produced by FastQC (version 0.11.5)