FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819319
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91260.5016162641076138No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC80790.4440672581334005No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC77330.42504915300725155No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGGATAAAACACT76290.41933272834505664No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC68390.3759098871610751No Hit
ATTATAATACTCCCCCCCTTTTCGGCCCTGGGACCAAGGTGGAGATCAAA65480.3599148912312794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA60120.33045331797227423No Hit
GTATTATAATAATGCTGACAGTAATAAACTGCCATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAAT57860.3180310874563504No Hit
GTAATAAACTGCCATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA56520.3106656941415991No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC56020.3079174130540054No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG53700.2951653888075703No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT53670.2950004919423147No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT53580.2945058013465478No Hit
GTTTTATCCAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA53390.29346145453326217No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52160.2867006830577815No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC52010.2858761987315034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51190.2813690177478496No Hit
CTCCTAAGGTTCTCATTGTCTGGGCATCTGCCCGGGAATCCGGGGTCCCT47970.26367008754374577No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC47020.2584483534773176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG45650.2509180632973107No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT43140.23712169223758997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42610.2342085142847406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42340.23272444249743998No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGC41500.22810733027028243No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40360.22184124939056865No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40120.22052207446852365No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATC40010.21991745262925302No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG39880.21920289954647865No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39800.21876317457246364No Hit
GGATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38390.21101302190544924No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38280.2104084000661786No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37970.2087044657918705No Hit
TTATAATACTCCCCCCCTTTTCGGCCCTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT37850.20804487833084798No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG37770.20760515335683294No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG37720.20733032524807357No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCAGGGCCGAAAAGG37540.20634094405653983No Hit
GTTTATTACTGTCAGCATTATTATAATACTCCCCCCCTTTTCGGCCCTGGGACCAAGGTGGAGATCAAACGAA35660.1960074071671873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35500.19512795721915727No Hit
GTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGACAATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA35470.19496306035390165No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGA32610.17924289253286532No Hit
GTCTGAAGATATGGCAGTTTATTACTGTCAGCATTATTATAATACTCCCC32610.17924289253286532No Hit
AACCAGGACAGCCTCCTAAGGTTCTCATTGTCTGGGCATCTGCCCGGGAA32560.17896806442410595No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA32430.17825351134133158No Hit
TCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC31520.1732516397619109No Hit
CAGTAATAAACTGCCATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA30640.16841466504774588No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29560.16247837789854336No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC29310.16110423735474647No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA29090.15989499367620522No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT28350.15582753766656643No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC28340.15577257204481457No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC28240.1552229158272958No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28100.15445339712276956No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC27220.14961642240860454No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC27110.1490118005693339No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT26990.14835221310831143No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC26920.14796745375604828No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26870.1476926256472889No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG26710.1468131756992589No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26040.14313047904188325No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC25900.142360960337357No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25770.1416464072545826No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25740.141481510389327No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCAGGGCCGAAAAGGGGGGGAGTATT25720.14137157914582324No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATCCAGCTCCGACAATAAGAACTA25640.14093185417180823No Hit
TGCCCAGACAATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACC25220.13862329805822948No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT23970.13175259533924508No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATATGGCAGTTTATTACTGTCAGCATTATTATAATACT23830.1309830766347188No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC23760.1305983172824557No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG23650.12999369544318506No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23610.12977383295617756No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23520.1292791423604107No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22960.12620106754230567No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22490.12361768331996753No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT22390.12306802710244877No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22330.12273823337193752No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22250.12229850839792253No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG22120.12158395531514815No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT21960.12070450536711813No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21870.12020981477135126No Hit
GTCTGGGCATCTGCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAG21540.11839594925353938No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21380.11751649930550936No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21340.11729663681850186No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21140.11619732438346436No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA21130.11614235876171249No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20640.11344904329587059No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20640.11344904329587059No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20570.11306428394360747No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG20410.11218483399557747No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20270.1114153152910512No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19640.10795248112068308No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19620.10784254987717933No Hit
ATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGAC19570.10756772176841994No Hit
CTCCTGTGCAGTCTCTGGCTTCAGCTTCAGTAATGATTTCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG19540.1074028249031643No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTTCTCATTGTCTGGGCATCTGCCCGGGAATC19400.10663330619863808No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA19310.10613861560287118No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC19240.10575385625060806No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19090.10492937192432993No Hit
ATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTA19040.10465454381557056No Hit
GCTAAGTAGTTCTTATTGTCGGAGCTGGATAAAACACTCTGGCTGGACTT18930.10404992197629993No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG18870.10372012824578866No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18750.10306054078476616No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18660.10256585018899929No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18630.10240095332374366No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18510.10174136586272116No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATATGGCAGTTTATTACTGTCAGCATTATTATAATACTCCCCCCCTTTT18500.10168640024096928No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18400.10113674402345053No Hit
GTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCAGAGATCCAGAGCAACAGAGAAATGAAGACCTGGGT18330.10075198467118741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT502.571778E-455.17863570
CGATTTA200.00802434250.63049325
CGATGGA200.00808572550.53297815
AATGGTA6400.045.26372570
GGTATCA12150.042.4367561
CGTAAGA350.001380006739.24089439
CAAACGT350.001433307238.9395135
GTGGTAT3600.038.3803021
GTATCAA29850.035.4496841
GCGGGTA400.002425802934.98225848
ACGTAAG400.002687185534.25988838
GACAGGC2100.033.6997761
GTGCTAG1400.033.6997761
TGGTATC4100.033.683092
GACAGGT1550.032.612691
ACAGACG10500.032.1875470
AATACCC450.003734870832.03343658
TATCAAC33700.031.2842942
CTAGAGA1400.031.2719944
TCCGAGA7750.031.14923170
Produced by FastQC (version 0.11.5)