FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774420
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23770.3069393868959996No Hit
GTGTATTACTGTCTGCAGTCTGGTATGTCTCCATTCTCTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAA22160.286149634565223No Hit
GTAATACACAGCAACATCTTCAGGCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA21620.27917667415614267No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC21060.27194545595413344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20560.2654890111309109No Hit
GTATTACTGTCTGCAGTCTGGTATGTCTCCATTCTCTTTCGGCGGAGGGA19820.2559334727925415No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG19700.2543839260349681No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18570.23979236073448518No Hit
CAGTAATACACAGCAACATCTTCAGGCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA17140.2213269285400687No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16510.21319180806280827No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15690.20260323855272336No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTTCTTAGCCTGGTATC15410.1989876294517187No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14920.19266031352496063No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14910.19253118462849617No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14390.18581648201234474No Hit
GTCTGGTATGTCTCCATTCTCTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCAT14370.1855582242194158No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12930.1669636631285349No Hit
ATACCAGACTGCAGACAGTAATACACAGCAACATCTTCAGGCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTC12880.16631801864621265No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12830.16567237416389038No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGAG12810.16541411637096148No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12380.1598615738229901No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12370.15973244492652566No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12100.1562459647219855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11900.15366338679269645No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11550.1491438754164407No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCC11510.1486273598305829No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGATTGGAGCCTGAAGATGTTGCTGTGTATTACTGTCTGCAGT11030.14242917280028924No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGACGCCAGTGGCCCTCTT10810.13958833707807133No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT10770.13907182149221353No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10570.1364892435629245No Hit
GTAATAGACGGCCGTGTCTTCGACTGTCAGGCTGTTCATTTGCAGATACA10450.1349396968053511No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATG10360.13377753673717102No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10270.132615376668991No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9980.12887063867152193No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9740.1257715451563751No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9500.12267245164122827No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9030.11660339350739908No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8740.11285865550993No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8640.11156736654528551No Hit
CTGCTACTCTGGCTCCCAGATGCTAGCGGAGAAATCGTGTTGACACAGTC8560.1105343353735699No Hit
GTCAGAGTGTTGGCAGCAACTTCTTAGCCTGGTATCAGCAGAAGTCAGGC8440.1089847886159965No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8360.10795175744428087No Hit
GATTTCACTCTCACCATCAGCAGATTGGAGCCTGAAGATGTTGCTGTGTATTACTGTCTGCAGTCTGGTATGTCT8160.10536917951499186No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8120.10485266392913407No Hit
CCATAGTACATCCAAGAGGGCCACTGGCGTCCCAGACAGGATCAGTGGCAGTGGGTCTGGGACAGATTTCACTCT8080.10433614834327626No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCTAGCGGAGAAATCGTGTTGACACAGTCTC8060.10407789055034737No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8020.10356137496448956No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7920.10227008599984504No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGTCTGGAGACTGTGTCAACACGATTTCTCCG7890.10188269931045169No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7860.10149531262105833No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7750.10007489475994938No Hit
GGATCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGA7750.10007489475994938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC1900.048.11229728
TCGTATG1800.047.9976740
TAGCGCT2000.045.76604529
ACTAGCG2000.045.65042527
CGTATGC2050.043.9435241
CCCACGA2100.043.3530419
ATCTCGT1900.043.31711237
TACACAT2200.042.8985065
CGAGACT2250.041.97501823
CGCTCAT2200.041.77913332
ACGAGAC2200.041.39046522
GACTAGC2250.040.5387726
GCGCTCA2300.039.92359531
CGTCTTC2050.039.438548
CACGAGA2400.037.93635621
TCCGAGC2400.037.92165813
TTATACA2500.037.7506832
TATACAC2600.037.5951163
CTTATAC2550.037.0224271
GTATGCC2450.036.8399742
Produced by FastQC (version 0.11.5)