FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784831
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46810.5964341367759428No Hit
GCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCGCATGATCAGTACTACTATCCCACAC33680.42913697343759355No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG32000.40773109115210787No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27830.35459863333634883No Hit
GTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTC27650.3523051459486182No Hit
TAGTAGTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCC25530.3252929611597911No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25440.3241462174659258No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTC23320.29713403267709865No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22900.29178256210572723No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22620.2882149150581463No Hit
GCCATGGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT22370.28502951590852044No Hit
CCCCAGGACAGACGGCCAAAATTACCTGTGGGGGAAGCAACATTGGAGGT20270.25827216305166334No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18830.2399242639498185No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18800.2395420160518634No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18760.23903235218792326No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17470.2225956925758539No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16470.20985409597735052No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16430.2093444321134104No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16210.20654128086173965No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16090.20501228926991924No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16010.20399296154203897No Hit
GTGTTGGTCGTCTATGATGATACCGACCGGCCCTCAGAGATCCCTGAGCG15740.20055273046044306No Hit
GGATAGTAGTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC15580.19851407500468252No Hit
GTATCATCATAGACGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTA15450.19685766744687708No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15260.19443676409316146No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15220.19392710022922133No Hit
CCCCTGTGTTGGTCGTCTATGATGATACCGACCGGCCCTCAGAGATCCCT15100.19239810863740092No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14850.18921270948777508No Hit
GTAGTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTC14660.18679180613405943No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14660.18679180613405943No Hit
TGATGATACCGACCGGCCCTCAGAGATCCCTGAGCGATTCTCTGGCTCGAACTCTGGGAACACGGCCACCCTGAC14380.1832241590864785No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACCT14290.18207741539261318No Hit
CTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTCGAC14120.1799113439708676No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13980.17812752044707714No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13870.17672594482124176No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATAGCTGTCAGGTGTGGGATAGTAGTA13670.1741776255015411No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAAATTAC13480.17175672214782545No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13440.1712470582838853No Hit
CTTTTACCTCCAATGTTGCTTCCCCCACAGGTAATTTTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG12970.16525850788258872No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC12850.16372951629076832No Hit
GTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC12670.16143602890303774No Hit
CAGTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG12470.15888770958333706No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA12270.15633939026363639No Hit
GGGTGGCCGTGTTCCCAGAGTTCGAGCCAGAGAATCGCTCAGGGATCTCTGAGGGCCGGTCGGTATCATCATAGA12200.15544747850174115No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12100.1541733188418908No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12040.1534088230459806No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12030.15328140707999557No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11680.1488218482705194No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTT11670.14869443230453436No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11630.14818476844059422No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGT11280.14372520963111804No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11260.14347037769914797No Hit
ATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTCGACCCTGC11120.1416865541753575No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10890.13875598695770172No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGGC10860.13837373905974662No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGG10770.13722699536588132No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10600.13506092394413574No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10250.13060136513465956No Hit
GTTTGGTCCCTCCGCCGAAGACCGCATGATCAGTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCC10190.12983686933874936No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGATGATACCGACCGGCCCTCAGA10130.12907237354283915No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10120.12894495757685412No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAAATTACCTGTGGGGGAAGCAACATTGGAGGTAAAAGTGTG10100.12869012564488405No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10080.12843529371291398No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG9730.12397573490343781No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9710.12372090297146775No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9620.12257415927760244No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAA9550.1216822475157072No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9520.1212999996177521No Hit
GCTATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG9500.12104516768578204No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9420.12002583995790177No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTC9410.11989842399191673No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9200.11722268870623102No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9130.11633077694433579No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9110.11607594501236572No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9070.11556628114842558No Hit
GGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8790.11199863410084464No Hit
AGGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGT8640.11008739461106913No Hit
GATCAGTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCG8640.11008739461106913No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG8610.10970514671311403No Hit
GTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA8500.10830357108727866No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8430.10741165932538342No Hit
GACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCGCATGATCAGTA8400.10702941142742832No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8380.10677457949545825No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8350.10639233159750315No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC8270.10537300386962288No Hit
TCTATGATGATACCGACCGGCCCTCAGAGATCCCTGAGCGATTCTCTGGCTCGAACTCTGGGAACACGGCCACC8180.10422626017575758No Hit
GGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC8180.10422626017575758No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT8130.10358918034583241No Hit
GCCCTCAGAGATCCCTGAGCGATTCTCTGGCTCGAACTCTGGGAACACGG8110.10333434841386234No Hit
GGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8110.10333434841386234No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8050.10256985261795214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.10155052489007187No Hit
ATGATGATACCGACCGGCCCTCAGAGATCCCTGAGCGATTCTCTGGCTCGAACTCTGGGAACACGGCCACCCTGA7950.1012956929581018No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC7950.1012956929581018No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7930.10104086102613173No Hit
CAGAGATCCCTGAGCGATTCTCTGGCTCGAACTCTGGGAACACGGCCACC7870.10027636523022153No Hit
GGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC7860.1001489492642365No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC7850.10002153329825146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC150.002007803471.8332262
CGCACTT150.002479355568.1122717
GAGTGCG150.00247998268.1079257
TCTCGTA800.047.28207838
CGTATGC1350.040.9790141
GTCGGTC504.1987623E-640.8673620
GTGGTAT1950.040.1815721
ATCTCGT955.456968E-1239.7704337
GATCTCG955.456968E-1239.72193536
GTATCAA24600.038.359981
TCGTATG1450.038.09619540
AATGGTA4300.037.61343870
AGCGATC1001.0913936E-1137.61031733
TATACAC1750.036.9752273
TGGTATC2200.035.6041372
TATCAAC26650.035.5257232
ACGAGAC1750.035.031422
ATGCCGT1400.034.70625344
AATTGCG400.00268317534.26791835
TAAGACA400.002765991334.0561334
Produced by FastQC (version 0.11.5)