FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784831
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44960.5728621830687116No Hit
GCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCGCATGATCAGTACTACTATCCCACAC32920.419453360022731No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG29980.3819930660231311No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27480.35013907452687265No Hit
GTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTC27180.34631659554732164No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25510.32503812922782105No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23190.2954776251192932No Hit
TAGTAGTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCC22480.2864310915343558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22150.2822263646568497No Hit
GCCATGGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT21990.28018770920108915No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTC20990.2674461126025858No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20240.25788991515370824No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19820.2525384445823369No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19030.24247258326951918No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18780.23928718411989333No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18480.23546470514034232No Hit
CCCCAGGACAGACGGCCAAAATTACCTGTGGGGGAAGCAACATTGGAGGT17590.2241246841676743No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15970.20348329767809883No Hit
GGGTGGCCGTGTTCCCAGAGTTCGAGCCAGAGAATCGCTCAGGGATCTCTGAGGGCCGGTCGGTATCATCATAGA15780.2010623943243832No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15620.19902373886862265No Hit
GGATAGTAGTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC15390.19609317165096687No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15340.1954560918210417No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15090.1922706926714159No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14770.1881933817598948No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14760.18806596579390977No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14660.18679180613405943No Hit
GTATCATCATAGACGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTA14490.18462573471231386No Hit
GTAGTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTC14240.18144033556268802No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14100.17965651203889754No Hit
GTGTTGGTCGTCTATGATGATACCGACCGGCCCTCAGAGATCCCTGAGCG13960.17787268851510707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13930.17749044061715197No Hit
CCCCTGTGTTGGTCGTCTATGATGATACCGACCGGCCCTCAGAGATCCCT13760.1753243691954064No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAAATTAC13680.17430504146752612No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13470.1716293061818404No Hit
CTTTTACCTCCAATGTTGCTTCCCCCACAGGTAATTTTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG13420.17099222635191524No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACCT13110.1670423314063792No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATAGCTGTCAGGTGTGGGATAGTAGTA12990.1655133398145588No Hit
TGATGATACCGACCGGCCCTCAGAGATCCCTGAGCGATTCTCTGGCTCGAACTCTGGGAACACGGCCACCCTGAC12850.16372951629076832No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTT12810.1632198524268282No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12720.1620731087329629No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12690.1616908608350078No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC12670.16143602890303774No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12580.16028928520917243No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12580.16028928520917243No Hit
CAGTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG12310.15684905412757652No Hit
CTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTCGAC12170.15506523060378605No Hit
GTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC12070.1537910709439357No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11730.14945892810044456No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11670.14869443230453436No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11620.1480573524746092No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGG11140.14194138610732757No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA11050.14079464241346226No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGGC10960.13964789871959696No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10930.13926565082164186No Hit
GTTTGGTCCCTCCGCCGAAGACCGCATGATCAGTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCC10720.13658991553595615No Hit
GGTGGTGCCTCAGCCATGGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC10530.1341690121822405No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10410.1326400205904201No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10310.13136586093056976No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGATGATACCGACCGGCCCTCAGA10180.12970945337276432No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGT10160.12945462144079425No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10120.12894495757685412No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAA10080.12843529371291398No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9920.12639663825715347No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9790.12474023069934802No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9790.12474023069934802No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9680.12333865507351265No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAAATTACCTGTGGGGGAAGCAACATTGGAGGTAAAAGTGTG9390.11964359205994667No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9310.1186242643320664No Hit
ATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCGGCTTCGACCCTGC9250.11785976853615619No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9010.11480178535251538No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9010.11480178535251538No Hit
GATCAGTACTACTATCCCACACCTGACAGCTATAGTCGGCCTCATCCCCG8950.11403728955660518No Hit
GGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8790.11199863410084464No Hit
GTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA8770.11174380216887457No Hit
AGGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGT8590.10945031478114396No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG8590.10945031478114396No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTC8510.10843098705326369No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8490.10817615512129362No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8470.10792132318932356No Hit
GTGTGGGATAGTAGTACTGATCATGCGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGG8440.10753907529136846No Hit
GGCCTGGGCCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC8420.10728424335939839No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT8390.10690199546144329No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8360.10651974756348818No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8290.10562783580159295No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8160.10397142824378751No Hit
GCTATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG8110.10333434841386234No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGGTCGTCTATGATGAT8020.10218760471999704No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7970.10155052489007187No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7930.10104086102613173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG150.002491178568.0305910
TCGGTCG200.006155660454.13813464
TACGTAT756.258815E-753.86498670
AAACGTT200.006290111753.8432562
TGGTATC2300.050.3091242
GTGGTAT2400.048.225211
GTATCAA22550.038.494621
TATTCTA750.002131473735.90999270
TATCAAC24400.035.008992
TGGTACG759.787582E-734.4737967
ATCAACG25050.034.0897063
TATAAGA807.9924575E-834.0326422
TCAACGC25150.033.9541634
CAACGCA25950.033.038515
AACGCAG26500.032.6095736
GGTACGT851.8420906E-631.79298868
GAGTTTA2800.030.3940371
CGCAGAG28700.030.346978
ACGCAGA28650.030.281187
AGAGTAC29050.029.97561311
Produced by FastQC (version 0.11.5)